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Development of analytical method for simultaneous detection of 1-[(2- chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) and 2- Hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH), two precursors of ketamine, in wastewater by LC-MS/MS

Tong Thi Thanh Vuong Truong Manh Cuong Nguyen Xuan Truong Nguyen Thi Thuy Linh Dang Thi Ngoc Lan Pham Thi Thanh Ha Tran Nguyen Ha Vu Ngan Binh
Published 09/24/2022

Article Details

How to Cite
Tong Thi Thanh Vuong, Truong Manh Cuong, Nguyen Xuan Truong, Nguyen Thi Thuy Linh, Dang Thi Ngoc Lan, Pham Thi Thanh Ha, Tran Nguyen Ha, Vu Ngan Binh. "Development of analytical method for simultaneous detection of 1-[(2- chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) and 2- Hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH), two precursors of ketamine, in wastewater by LC-MS/MS". Vietnam Journal of Food Control. vol. 5, no. 3, pp. 451-461, 2022
PP
451-461
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296

Main Article Content

Abstract

Ketamin is a pharmaceutical compound that is abused as a narcotic drug, causing health problems for users. In controlling ketamine abuse, the detection of ketamine precursors can be a useful tool for tracing illegal ketamine production sites. In this study, an analytical method for determination of two ketamine precursors, 1-[(2-chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) and 2-Hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH), in wastewater using solid phase extraction (SPE) and liquid chromatogrphy tandem mass spectrometry (LC-MS/MS) was developed and validated. An LC-MS/MS system employing a Zorbax C18 column (2.1 x 100 mm, 1.8 µm) and a mobile phase composing acetonitrile and water containing 0.1% (m/v) of formic acid in a gradient program at the flow rate of 0.3 mL/min and injection volume of 5 µL. The detection of analytes was done with electrospray ionization at positive mode (ESI+) employing multi reaction monitoring (MRM) at m/z of 238.5 and 225.5 for for CCM and HCH, respectively. The method was validated according to requirements of AOAC International and EC and was proved as reliable for intended use.

Keywords:

ketamin precursor, determination, wastewater, LC-MS/MS, SPE

References

[1]. C. Yiu-Cheung, “Acute and chronic toxicity pattern in ketamin abusers in Hong Kong,” Journal of Medical Toxicology, vol. 8, no.3, pp. 267-270, 2012.
[2]. T. Okon, “Ketamin: An introduction for the pain and palliative medicine physician,” Pain Physician, vol. 10, no.3, pp. 493-500, 2007.
[3]. I. Dimitrov, W. Denny, and J. Jose, “Syntheses of Ketamin and Related Analogues: A Mini Review,” Synthesis, vol.50, no. 21, A-O, 2018.
[4]. AOAC International, Appendix F: Guidelines for standard method performance requirements, AOAC International Official Methods of Analysis, 2012.
[5]. European Commission, Version 1.1, 25 November 2021, EURL Guidance Document on Confirmation Method Validation, 2021.

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