Bìa tạp chí

 

009bet

Determination of glucosamine in dietary supplement samples by capillary electrophoresis using capacitively coupled contactless conductivity detector (CE-C4D)

Dang Thi Huyen My Nguyen Thi Ngoc Ly Do Yen Nhi Vu Tung Lam Dinh Thi Diu Kieu Thi Lan Phuong Hoang Quoc Anh Tran Cao Son Nguyen Thi Minh Thu Nguyen Quang Huy Nguyen Thi Anh Huong
Received: 04 May 2022
Revised: 04 Jun 2022
Accepted: 04 Jun 2022
Published: 05 Oct 2022

Article Details

How to Cite
Dang Thi Huyen My, Nguyen Thi Ngoc Ly, Do Yen Nhi, Vu Tung Lam, Dinh Thi Diu, Kieu Thi Lan Phuong, Hoang Quoc Anh, Tran Cao Son, Nguyen Thi Minh Thu, Nguyen Quang Huy, Nguyen Thi Anh Huong. "Determination of glucosamine in dietary supplement samples by capillary electrophoresis using capacitively coupled contactless conductivity detector (CE-C4D)". Vietnam Journal of Food Control. vol. 5, no. 3, pp. 243-255, 2022
PP
243-255
Counter
784

Main Article Content

Abstract

The quantification of glucosamine in dietary supplements was investigated by using capillary with capacitively coupled contactless conductivity detection (CE-C4D). Optimal conditions included electrophoresis buffer solution 10 mM Tris/Ace; pH = 5,0; sample injection height 25 cm; sample injection time 30 s; separation voltage 20 kV. The limit of detection (LOD) and the limit of quantification (LOQ) of glucosamine were 8.00 ppm and 26.67 ppm, respectively. Calibration curve was constructed over the range from 20 to 1000 ppm giving a good linear correlation coefficient (R2 = 0.9993). The relative standard deviation (RSD) ranged from 1.28% to 2.33% (n = 3) and the recovery efficiency ranged from 95.2 to 102.3% which indicated good precision and trueness of our method. The method was applied to determine glucosamine in 4 dietary supplement samples randomly collected at the local markets. The CE-C4D results were compared with those obtained by reference HPLC-FLD method, showing good agreement between the two methods (relative error < 15%).

Keywords:

Glucosamine, CE-C4D, dietary supplements

References

[1]. J. W. Anderson, R. J. Nicolosi, and J. F. Borzelleca, “Glucosamine effects in humans: a review of effects on glucose metabolism, side effects, safety considerations and efficacy,” Food and Chemical Toxicology, vol. 43, no. 2, pp. 187- 201, 2005.
[2]. Jean-Yves Reginster, Audrey Neuprez, Marie-Paule Lecart, Nathalie Sarlet, Olivier Bruyere, “Role of glucosamine in the treatment for osteoarthritis,” Rheumatology International, vol. 32, pp. 2959-2967, 2012.
[3]. J. Y. Reginster, R. Deroisy, L. C. Rovati, R. L. Lee, E. Lejeune, O. Bruyere, G. Giacovelli, Y. Henrotin, J. E. Dacre and C. Gossett, “Long-term effects of glucosamine sulphate on osteoarthritis progression: a randomised, placebo-controlled clinical trial,” The Lancet, vol. 357, no. 9252, pp. 251-256, 2001.
[4]. Helmick CG, Pollard RA, “Evidence for under-reading of radiographic osteoarthritis of the hips and knees in the First National Health and Nutrition Examination Survey (NHANES I),’’ Arthritis Rheum, vol. 37 (Suppl.), no. S301, 1994.
[5]. Houpt JB, McMillan R, Wein C, Paget-Dellio SD, “Effect of glucosamine hydrochloride in the treatment of pain of osteoarthritis of the knee,” The Jourmal of Rheumatology, vol. 26, no. 11, pp. 2423-2430, 1999.
[6]. Pham Thi Mai Huong, Truong Thi My Hanh, Dang Thi Huyen My, Vu Thi Trang, Cao Cong Khanh, Hoang Quoc Anh, Mai Thi Ngoc Anh, Nguyen Thi Minh Thu, Nguyen Quang Huy, Nguyen Thi Anh Huong, “Determination of glucosamine in functional food samples by high performance liquid chromatography (HPLC-FLD),” Vietnam Journal of Food Control, volume 5, no. 1, p. 11-24, 2022.
[7]. T. Sriveena, A. Srividya, A. Ajitha and V. Uma Maheswara Rao, “RP-HPLC Method Development and Validation for Simultaneous Estimation of Diacerein and Glucosamine in bulk and Pharmaceutical dosage form,” World Journal of Pharmaceutical Research, vol. 4, iss. 8, pp. 2349-2360, 2015.
[8]. S. Pullareddy and C. Rambabu, “Simultaneous determination of Glucosamine and Diacerein in pharmaceutical dosage form by RP-HPLC,” International Journal of Pharmacy and Pharmaceutical Research, vol. 2, no. 2, pp. 139-151, 2015.
[9]. Kesharwani Saurabh, Kohali Dharamveer, “Development and validation of analytical methods for simultaneous estimation of diacerein and aceclofenac in bulk and tablets using UV Visible spectroscopy,” International Journal of Chem Tech Research, vol. 2, no. 3, pp. 1816-1822, 2010.
[10]. Václavíková E., Kvasnička F, “Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements,” Czech Journal of Food Science, vol. 31, pp. 55-65, 2013.
[11]. Nicola Volpi, “Capillary electrophoresis determination of glucosamine in nutraceutical formulation after labeling with anthranilic acid and UV detection,” Journal of Pharmaceutical and Biomedical Analysis, vol. 49, pp. 868- 871, 2009.
[12]. S. Akamatsu and T. Mitsuhashi, “Development of a simple capillary electrophoretic determination of glucosamine in nutritional supplements using in-capillary derivatisation with o-phthalaldehyde,” Food Chemistry, vol. 130, no. 4, pp. 1137-1141, 2012.
[13]. Le Thi Hong Hao, Pham Thi Ngoc Mai, Nguyen Thi Anh Huong, Nguyen Van Anh, Pham Tien Duc, Vu Thi Trang, Application of electrophoresis in food analysis, Science and Technics Publishing House, 2016.
[14]. Thi Anh Huong Nguyen et. al, “Simple semi-automated portable capillary electrophoresis instrument with contactless conductivity detection for the determination of β-agonists in pharmaceutical and pig-feed samples”, Journal of Chromatography A, vol. 1360, pp. 305-311, 2014.
[15]. Patcharin Chaisuwan, Thitiya Kongprasertsak, Areeporn Sangcakul, Norman W Smith, Duangjai Nachapricha, Prapin Wilairat, Kanchana Uraisin, “Direct injection of human serum and pharmaceutical formulations for glucosamine determination by CE-C(4)D method,’’ Journal of Chromatography B, vol. 879, pp. 2185-2188, 2011.
[16]. AOAC Official Methods of Analysis, Appendix F: Guidelines for standard method performance requirements, 2012.

 Submit