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Determination of glucosamine in dietary supplement samples by capillary electrophoresis using capacitively coupled contactless conductivity detector (CE-C4D)

Dang Thi Huyen My Nguyen Thi Ngoc Ly Do Yen Nhi Vu Tung Lam Dinh Thi Diu Kieu Thi Lan Phuong Hoang Quoc Anh Tran Cao Son Nguyen Thi Minh Thu Nguyen Quang Huy Nguyen Thi Anh Huong
Published 06/04/2022

Article Details

How to Cite
Dang Thi Huyen My, Nguyen Thi Ngoc Ly, Do Yen Nhi, Vu Tung Lam, Dinh Thi Diu, Kieu Thi Lan Phuong, Hoang Quoc Anh, Tran Cao Son, Nguyen Thi Minh Thu, Nguyen Quang Huy, Nguyen Thi Anh Huong. "Determination of glucosamine in dietary supplement samples by capillary electrophoresis using capacitively coupled contactless conductivity detector (CE-C4D)". Vietnam Journal of Food Control. vol. 5, no. 3, pp. 243-255, 2022
PP
243-255
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508

Main Article Content

Abstract

The quantification of glucosamine in dietary supplements was investigated by using capillary with capacitively coupled contactless conductivity detection (CE-C4D). Optimal conditions included electrophoresis buffer solution 10 mM Tris/Ace; pH = 5,0; sample injection height 25 cm; sample injection time 30 s; separation voltage 20 kV. The limit of detection (LOD) and the limit of quantification (LOQ) of glucosamine were 8.00 ppm and 26.67 ppm, respectively. Calibration curve was constructed over the range from 20 to 1000 ppm giving a good linear correlation coefficient (R2 = 0.9993). The relative standard deviation (RSD) ranged from 1.28% to 2.33% (n = 3) and the recovery efficiency ranged from 95.2 to 102.3% which indicated good precision and trueness of our method. The method was applied to determine glucosamine in 4 dietary supplement samples randomly collected at the local markets. The CE-C4D results were compared with those obtained by reference HPLC-FLD method, showing good agreement between the two methods (relative error < 15%).

Keywords:

Glucosamine, CE-C4D, dietary supplements

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