Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
This study aimed to establish the analytical parameters and validate a high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method for the determination of aflatoxins (AFB1, AFB2, AFG1, and AFG2) in food samples. The validated method used to assess aflatoxin contamination in cereals, coffee products, and dried seafood collected in Hanoi. Positive samples were further confirmed by liquid chromatography–tandem mass spectrometry (LC-MS/MS), and the quantitative results obtained by the two methods were compared. The validated method demonstrated good specificity, with limits of detection (LOD) and quantification (LOQ) of 0.5 and 1.0 μg/kg, respectively, for each aflatoxin. The intermediate precision (RSD) ranged from 2.55 to 18.4%, while recoveries ranged from 70.1% to 119%. The evaluation results demonstrated that the method was reliable for the determination of aflatoxins at low concentrations in compliance with the requirements of QCVN 8-1:2011/BYT and showed good agreement with LC-MS/MS for quantitative analysis. The validated method was applied to the analysis of 100 food samples, including cereals, coffee, and dried seafood. Five samples (5.0%) were found to be contaminated with total aflatoxins at concentrations ranging from 9.04 to 726 µg/kg. These findings highlight the need to maintain and strengthen routine food safety monitoring programs for the effective control of aflatoxin contamination and the protection of public health.
Aflatoxin, cereals, Immunoaffinity, HPLC-FLD, LC-MS/MS.
[1]. L. T. P. Thao, Investigation of Aflatoxin Contamination and Development of Technological Solutions for Post-Harvest Peanut Storage in the Central and Northern Regions of Vietnam, doctoral dissertation, Hanoi University of Science and Technology, Hanoi, Vietnam, 2018 [In Vietnamese].
[2]. F. Kilicel, H. S. Karapinar, and A. Cimen, “Quantitation of aflatoxins in food materials using HPLCFLD method,” Science Journal of Analytical Chemistry, vol. 5, no. 6, pp. 90–97, 2017.
[3]. E. Yohannis, M. M. Urugo, T. A. Teka, P. Getachew, Y. B. Tola, S. F. Forsido, Y. S. Kebede, and T. F. Teferra, “Aflatoxin contamination in agri-food systems: A comprehensive review of toxicity, food security, economic impacts, and sustainable mitigation across the value chain,” Food Science & Nutrition, vol. 13, no. 10, Art. no. e71104, 2025.
[4]. A. Jallow, H. Xie, X. Tang, Z. Qi, and P. Li, “Worldwide aflatoxin contamination of agricultural products and foods: From occurrence to control,” Comprehensive Reviews in Food Science and Food Safety, vol. 20, no. 3, pp. 2332–2381, 2021.
[5]. Ministry of Health of Vietnam, QCVN 8-1:2011/BYT: National Technical Regulation on the Limits of Mycotoxins Contamination in Food, Hanoi, Vietnam: Ministry of Health of Vietnam, 2011.
[6]. D. H. Tuan, Assessment of Contamination and Health Risks Associated with Mycotoxins in Foods in Selected Northern Provinces of Vietnam, doctoral dissertation, Hanoi University of Pharmacy, Hanoi, Vietnam, 2020 [In Vietnamese].
[7]. European Food Safety Authority, Aflatoxins (Sum of B1, B2, G1, G2) in Cereals and Cereal-Derived Food Products, EFSA Supporting Publications, vol. 10, no. 3, EN-406, Parma, Italy: European Food Safety Authority, 2013.
[8]. S. Bakırdere, S. Bora, E. G. Bakırdere, F. Aydın, Y. Arslan, O. T. Komesli, I. Aydın, and E. Yıldırım, “Aflatoxin species: Their health effects and determination methods in different foodstuffs,” Central European Journal of Chemistry, vol. 10, no. 3, pp. 675–685, 2012.
[9]. F. M. Trombete, T. B. Santos, G. M. Direito, M. E. Fraga, and T. Saldanha, “In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products,” Pesquisa Agropecuária Tropical, vol. 44, no. 3, pp. 255–262, 2014.
[10]. Ministry of Health, Labour and Welfare of Japan, Test Methods for Total Aflatoxin, Notification No. ShokuAn 0816-2, Tokyo, Japan: Ministry of Health, Labour and Welfare of Japan, 2011.
[11]. Ministry of Science and Technology of Vietnam, TCVN 12625:2019: Foodstuffs-Determination of Aflatoxins B1, B2, G1, G2, Total Aflatoxin and Ochratoxin A in Ginseng and Ginger-Liquid
Chromatographic Method Using Immunoaffinity Column Cleanup, Hanoi, Vietnam: Ministry of Science and Technology of Vietnam, 2019 [In Vietnamese].
[12]. Ministry of Science and Technology of Vietnam, TCVN 7930:2008: Foodstuffs-Determination of Aflatoxin B1 and the Sum of Aflatoxins B1, B2, G1 and G2 in Cereals, Shell-Fruits and Derived Products-High Performance Liquid Chromatographic Method with Post-Column Derivatization and Immunoaffinity Column Clean-Up, Hanoi, Vietnam: Ministry of Science and Technology of Vietnam, 2008.
[13]. AOAC International, “AOAC Official Method 991.31: Aflatoxins in corn, raw peanuts, and peanut butter: Immunoaffinity column (Aflatest) method,” in Official Methods of Analysis of AOAC International, 22nd ed., G. W. Latimer, Jr., Ed. Rockville, MD, USA: AOAC International, pp. C49-21C49-22, 2023.
[14]. C. M. Weaver and M. W. Trucksess, “Determination of aflatoxins in botanical roots by a modification of AOAC Official MethodSM 991.31: Single-laboratory validation,” Journal of AOAC International, vol. 93, no. 1, pp. 184–189, 2010.
[15]. VICAM, AflaTest Fluorometer Instruction Manual, Cat. No. 715001734, Milford, MA, USA: VICAM, a Waters Business, 2014.
[16]. AOAC International, “Appendix F: Guidelines for Standard Method Performance Requirements,” in Official Methods of Analysis of AOAC InternationaL, 22nd ed., G. W. Latimer, Jr., Ed. Rockville, MD, USA: AOAC International, 2023.
[17]. Shimadzu Corporation, “Quantitation by HPLC and identification by LC/MS for total aflatoxins in food,” Application News, no. L435, 2012.
[18]. European Committee for Standardization, EN 14123:2003: Foodstuffs-Determination of Aflatoxin B1 and the Sum of Aflatoxin B1, B2, G1 and G2 in Peanuts, Pistachios, Figs and Paprika Powder-High Performance Liquid Chromatographic Method with Post-Column Derivatization and Immunoaffinity Column Clean-Up. Brussels, Belgium: European Committee for Standardization, 2003.
[19]. N. V. Thuan, D. Oanh, N. T. T. Mai, and D. T. Khuyen, “Surveying the levels of mold and aflatoxin in maize and peanut in some households in Ea H’Leo, Cu M’Gra, Krong Pac districts in Daklak province in 2016,” Vietnam Journal of Food Control, vol. 5, no. 3, pp. 414–421, 2022.