Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
Taxiphyllin is a cyanogenic glycoside that, upon hydrolysis, produces hydrocyanic acid, which is toxic to humans. Several studies have detected taxiphyllin in hydrangea leaves and bamboo shoots. In this study, liquid chromatography-transcriptional mass spectrometry (LC-MS/MS) with negative-mode electron spray ionization was used to develop and validate a method for determining taxiphyllin in bamboo shoots. The mobile phase consisted of channel A, 0.1% formic acid in water, and channel B, 0.1% formic acid in acetonitrile; the chromatographic column was a Kinetex Coreshell C18 reversed-phase column (3.0 mm x 150 mm; 2.6 µm). The limit of detection (LOD) and limit of quantification (LOQ) for taxiphyllin were 0.15 mg/kg and 0.4 mg/kg, respectively. The calibration curve for taxiphyllin was established in the range of 5.0 – 100.0 ng/mL. The method has a recovery rate of 82.6 – 107.0%, the intra-day repeatability with a relative standard deviation of 4.74 – 7.46%, meeting the requirements of the Association of Official Analytical Collaboration (AOAC) 2016 and European regulations (EU 2021/808). The method was applied to analyze the taxiphyllin content in 8 bamboo shoot samples purchased in Hanoi.
Taxiphyllin, cyanogenic glycoside, LC-MS/MS.
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