Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
The presence of Escherichia coli (E. coli) on food contact surfaces is considered a significant risk to food safety and can have a major impact on public health. This study aimed to examine the contamination rate, antimicrobial resistance profiles, and several genes encoding virulence factors of E. coli recovered from the surfaces of pork-selling counters in wet markets in Gia Lam, Hanoi City. The findings in our study show that 50% (20/50) of swab samples carried E. coli. Resistance was most prevalent against tetracycline (75%), while ampicillin, streptomycin, and sulfamethoxazole/trimethoprim all demonstrated a slightly lower but identical resistance rate of 70%. The PCR test indicates that only one isolate (5%) harbored the stx2 gene and one (5%) carried the eae gene, while none of the isolated strains possessed stx1 and ehxA.
Food contact surface, antibiotic resistance, Escherichia coli, virulence genes, pork
[1]. D. Grace, “Food safety in low and middle income countries,” International Journal of Environmental Research and Public Health, vol. 12, no. 9, pp. 10490–10507, 2015.
[2]. J. J. Carrique-Mas and J. E. Bryant, “A review of foodborne bacterial and parasitic zoonoses in Vietnam,” EcoHealth, vol. 10, no. 4, pp. 465–489, 2013.
[3]. N. T. D. Nga et al., “Household pork consumption behaviour in Vietnam: Implications for pro-smallholder pig value chain upgrading,” International Livestock Research Institute (ILRI), Nairobi, Kenya, Project Report, 2015.
[4]. T. T. H. Le et al., “Food safety knowledge, needed and trusted information of pork consumers in different retail types in Northern Vietnam,” Frontiers in Sustainable Food Systems, vol. 6, p. 1063927, 2022.
[5]. F. Prestinaci, P. Pezzotti, and A. Pantosti, “Antimicrobial resistance: A global multifaceted phenomenon,” Pathogens and Global Health, vol. 109, no. 7, pp. 309–318, 2015.
[6]. H. M. Duc et al., “Antibiotic resistance ability of Escherichia coli isolated from pork and chicken meat at retail markets in Gia Lam district, Hanoi,” Journal of Veterinary Science and Technology, vol. XXIX, no. 6, pp. 41–47, 2022.
[7]. H. M. Duc, C. T. T. Ha, T. T. K. Hoa, L. Van Hung, N. Van Thang, and H. M. Son, “Prevalence, molecular characterization, and antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli isolated from raw beef, pork, and chicken meat in Vietnam,” Foods, vol. 13, no. 13, p. 2059, 2024.
[8]. M. A. Sebsibe and E. T. Asfaw, “Occurrence of multidrug-resistant Escherichia coli and Escherichia coli O157:H7 in meat and swab samples of various contact surfaces at abattoir and butcher shops in Jimma town, southwest district of Ethiopia,” Infection and Drug Resistance, vol. 13, pp. 3853–3862, 2020.
[9]. A. F. Beyi et al., “Prevalence and antimicrobial susceptibility of Escherichia coli O157 in beef at butcher shops and restaurants in central Ethiopia,” BMC Microbiology, vol. 17, no. 1, p. 49, 2017.
[10]. National Standard TCVN 8129:2009 (ISO 18593:2004) Microbiology of food and animal feeding stuffs - Horizontal methods for sampling techniques from surfaces using contact plates and swabs, Ministry of Science and Technology, Vietnam, 2009. [Online]. Available: https://caselaw.vn/van-ban-phap-luat/253512-tieu-chuan-quoc-gia-tcvn-8129-2009-iso-18593-2004-ve-vi-sinh-vat-trong-thuc-pham-va-thuc-an-chan-nuoi-phuong-phap-lay-mau-be-mat-su-dung-dia-tiep-xuc-va-lau-be-mat-nam-2009
[11]. C. Zhao et al., “Prevalence of Campylobacter spp., Escherichia coli, and Salmonella Serovars in Retail Chicken, Turkey, Pork, and Beef from the Greater Washington, D.C., Area,” Applied and Environmental Microbiology, vol. 67, no. 12, pp. 5431–5436, 2001.
[12]. Performance Standards for Antimicrobial Susceptibility Testing, 31st ed., CLSI supplement M100. Wayne, PA, USA: Clinical and Laboratory Standards Institute, 2021.
[13]. J. Bai, X. Shi, and T. G. Nagaraja, “A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7,” Journal of Microbiological Methods, vol. 82, no. 1, pp. 85–89, 2010.
[14]. J. Bai, Z. D. Paddock, X. Shi, S. Li, B. An, and T. G. Nagaraja, “Applicability of a multiplex PCR to detect the seven major Shiga toxin–producing Escherichia coli based on genes that code for serogroup-specific O-antigens and major virulence factors in cattle feces,” Foodborne Pathogens and Disease, vol. 9, no. 6, pp. 541–548, 2012.
[15]. L. H. Phong, V. M. Chau, N. M. Hieu, N. T. Thi, N. T. K. Cuc, and B. T. D. Hang, “Survey on the prevalence of Escherichia coli, Salmonella and residues of some antibiotics in pork and chicken in some provinces in the Western South of Vietnam,” Journal of Veterinary Science and Technology, vol. 16, no. 7, pp. 47–54, 2019.
[16]. L. M. Duc et al., “Assessment of microbial contamination levels in pork sold at some markets in Hue City,” Journal of Agriculture, Science and Technology, Hue University of Agriculture and Forestry, vol. 7, no. 3, pp. 3814–3821, 2023.
[17]. C. L. Little and S. K. Sagoo, “Evaluation of the hygiene of ready-to-eat food preparation areas and practices in mobile food vendors in the UK,” International Journal of Environmental Health Research, vol. 19, no. 6, pp. 431–443, 2009.
[18]. M. A. Bukhari et al., “Assessment of microbiological quality of food preparation process in some restaurants of Makkah city,” Saudi Journal of Biological Sciences, vol. 28, no. 10, pp. 5993–5997, 2021.
[19]. M. A. Sebsibe and E. T. Asfaw, “Occurrence of multidrug-resistant Escherichia coli and Escherichia coli O157:H7 in meat and swab samples of various contact surfaces at abattoir and butcher shops in Jimma town, southwest district of Ethiopia,” Infection and Drug Resistance, vol. 13, pp. 3853–3862, 2020.
[20]. M. M. S. Coelho et al., “Escherichia coli and Enterobacteriaceae Counts, Virulence Gene Profile, Antimicrobial Resistance, and Biofilm Formation Capacity during Pig Slaughter Stages,” Life, vol. 14, no. 10, p. 1261, 2024.
[21]. A. N. da C. E. Sampaio et al., “Escherichia coli Occurrence and Antimicrobial Resistance in a Swine Slaughtering Process,” Pathogens, vol. 13, no. 10, p. 912, 2024.
[22]. H. Li et al., “Prevalence of Escherichia coli and Antibiotic Resistance in Animal-Derived Food Samples - Six Districts, Beijing, China, 2020,” China CDC Weekly, vol. 3, no. 47, pp. 999–1004, 2021.
[23]. B. Li, H. Liu, and W. Wang, “Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli,” BMC Microbiology, vol. 17, no. 1, p. 215, 2017.
[24]. H. M. Duc, C. T. T. Ha, T. T. K. Hoa, and H. M. Son, “Detection of virulence genes (stx1, stx2, eae, ehxA) and antibiotic resistance of Escherichia coli isolated from meat,” Vietnam Journal of Agricultural Sciences, vol. 22, no. 5, pp. 606–614, 2024.