Capillary electrophoresis with capacitively coupled contactless conductivity detector (CE-C4D) is renowned for its numerous advantages, such as compact equipment, low cost of setup and operation, simplicity in functionality, and minimal use of samples and chemical solvents. This method has been applied across various fields, including environmental analysis, forensic science, biochemistry, pharmaceuticals, and food science. In this study, the CE-C4D method was applied to determine the presence of glucosamine, vitamin B6, and certain minerals in dietary supplements. Notably, glucosamine was simultaneously determined with calcium, and vitamin B6 with magnesium. The method was validated to meet the requirements of AOAC. The detection limits achieved for calcium, glucosamine, vitamin B6 and magnesium were 0.05 ppm; 0.50 ppm; 0.10 ppm and 1.00 ppm, respectively. The concurrent determination of active substances with different properties within a single product is crucial, optimizing analytical efficiency and yielding high accuracy for the analysis.
CE-C4D, vitamin B6, glucosamine, calcium, magneium, dietary supplements
[1]. A. Dickinson, J. Blatman, N. El-Dash, and J. C. Franco, “Consumer usage and reasons for using dietary supplements: report of a series of surveys,” Journal of the American College of Nutrition, vol. 33, no. 2, pp. 176-182, 2014.
[2]. G. B. der Voet, A. Sarafanov, T. I. Todorov, et al, “Clinical and analytical toxicology of dietary supplements: a case study and a review of the literature,” Biological Trace Element Research, vol. 125, no. 1, pp. 1-12, 2008.
[3]. WHO, FAO, “Vitamin and mineral requirements in human nutrition,” World Health Organization, 194-216, 2004.
[4]. H. Harmita, C.Jatmika, and M.I.Nugraha, "Determination of levels of glucosamine hydrochloride and chondroitin sulfate in mixtures in tablet and cream forms using high-performance liquid chromatography with fluorescence," International Journal of Applied Pharmaceutics, vol. 9, pp.144- 149, 2017.
[5]. A. M. Farag, M. S. Rizk, H. A. El-Bassel, and M. H. Youssif, “Fast and efficient RPHPLC method for simultaneous determination of water-soluble vitamins in some nutraceutical supplements,” Egyptian Journal of Chemistry, vol. 62, no. 12, pp. 2195–2211, 2019.
[6]. P. Gaonkar, V.Khanvilkar, R.Shettigar, and C.Gadgoli, "Spectrophotometric method for determination of glucosamine in tablets," Indian Journal of Pharmaceutical Sciences, vol. 68, no. 1, pp. 83-84, 2006.
[7]. A. Krejčová, D. Kahoun, T. Černohorský, and M. Pouzar, "Determination of macro and trace element in multivitamins preparations by inductively coupled plasma optical emission spectrometry with slurry sample introduction," Food Chemistry, vol. 98, pp. 171-178, 2006.
[8]. Thi Anh Huong Nguyen, Thi Ngoc Mai Pham, Thi Tuoi Doan, et al, “Simple semiautomated portable capillary electrophoresis instrument with contactless conductivity detection for the determination of β-agonists in pharmaceutical and pig-feed samples,” Journal of Chromatography A, vol. 1360, pp. 305-311, 2014.
[9]. G. W. Latimer, “AOAC Official Methods of Analysis- Appendix F: Guidelines for standard method performance requirements,” AOAC INTERNATIONAL, 2012.