Bìa tạp chí

 

009bet

Antibacterial activity and cytotoxicity lung cancer cells A549 of Ganoderma colossus

Che Thi Cam Ha Huynh Nhu Y Nguyen Trong Nghia Nguyen Minh Tri Nguyen Viet Thang Nguyen Thi Kim Hue
Received: 02 Jul 2024
Revised: 04 Sep 2024
Accepted: 06 Sep 2024
Published: 30 Sep 2024

Article Details

How to Cite
Che Thi Cam Ha, Huynh Nhu Y, Nguyen Trong Nghia, Nguyen Minh Tri, Nguyen Viet Thang, Nguyen Thi Kim Hue. "Antibacterial activity and cytotoxicity lung cancer cells A549 of Ganoderma colossus". Vietnam Journal of Food Control. vol. 7, no. 3, pp. 411-419, 2024
PP
411-419
Counter
25

Main Article Content

Abstract

Golden Ganoderma is a valuable species in the medicinal formula Luc Bao Linh Chi which has been used in traditional medicine in Vietnam. The golden Ganoderma mushroom sample discovered at Delonix regia tree in Hue city, Thua Thien Hue province was identified as Ganoderma colossus (Fr.) C.F. Baker. This study investigated the cytotoxicity of A549 lung cancer cells and the antibacterial activity of the aqueous extract from the fruiting body of golden Ganoderma. The extract at a concentration of 1 mg/mL showed the strongest inhibitory activity against strains of Bacillus subtilis (27.1 ± 0.36 mm), Staphylococcus aureus (14.2 ± 0.54 mm), Gram negative bacteria strains (Escherichia coli and Salmonella enteritidis) were weaker inhibited. The cytotoxic effect on lung cancer cells was determined by the MTT method [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide)]. The results showed that the extract at a concentration of 128 µg/mL had an inhibitory effect on A549 lung cancer cells of 86.36% with an IC50 value of 9.21 ± 0.8 µg/mL compared to the positive control Elipcitine of 0.34 ± 1.13 µg/mL.

Keywords:

Ganoderma colossus, antibacterial, lung cancer cells, MTT method

References

[1]. C. Sharma, N. Bhardwaj, A. Sharma, et al., “Bioactive metabolites of Ganoderma lucidum: Factors, mechanism and broad spectrum therapeutic potential,” Journal of Herbal Medicine, vol. 17-18, pp. 100268, 2019.
[2]. Do Tat Loi, Vietnamese medicinal plants and herbs, Hanoi: Science and Technology Publishing House, 1999 (In Vietnamese).
[3]. C. J. Weng, P. S. Fang, D. H. Chen, K. D. Chen, and G. C. Yen, “Anti-invasive effect of a rare mushroom, Ganoderma colossum on human hepatoma cells,” Journal of Agricultural and Food Chemistry, vol. 58, no. 13, pp. 7657-7663, 2010.
[4]. O. A.-H. M. Al-Bedak, A. M. Moharram, H. E.-D. F. Abdel-Raheem, S. L. Stephenson, and F. Ameen, “Nutritional composition, antioxidant activity, cytotoxicity and enzymatic potential of Ficus nitida associated Tomophagus colossus,” Agronomy, vol. 13, pp. 2850, 2023.
[5]. L. N. Ofodile, N. Uma, R. J. Grayer, O. T. Ogundipe, and M. S. J. Simmonds, “Antibacterial Compounds from the Mushroom Ganoderma colossum from Nigeria,” Phytotherapy Research, vol. 26, no. 5, pp. 748-751, 2011.
[6]. Nguyen Kim Phi Phung, Methods of isolation of organic compounds, Ho Chi Minh City National University Publishing House, 2007 (In Vietnamese).
[7]. T. J. White, T. D. Bruns, S.B. Lee, and J. W. Taylor, “Amplilcation and direct sequencing of fungal ribosomal RNA Genes for phylogenetics,” in PCR Protocols: A Guide to Methods and Applications, Academic Press, 1990.
[8]. A. W. Bauer, W. M. Kirby, J. C. Sherris, and M. Turck, “Antibiotic susceptibility testing by a standardized single disk method,” American Society of Clinical Pathologists, vol. 45, no. 4, pp. 493-496, 1966.
[9]. T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay,” Journal of immunological methods, vol. 65, pp. 55-63, 1983.
[10]. Trinh Tam Kiet, Vietnamese giant mushrooms, Hanoi: Science and Technology Publishing House, 2011 (In Vietnamese).
[11]. Nguyen Thi Dieu Hanh, Nguyen Ngoc An, Luu Van Luong, Nguyen Cong Van, Ho Nguyen Hoang Yen, Pham Tan Viet, “Anticancer and antibacterial activity of ganoderma lucidum and humphreya endertii from Phuoc Binh national park,” Journal of Science and Technology, Ho Chi Minh City University of Industry, vol. 39B, pp. 1-11, 2020 (In Vietnamese).
[12]. J. P. Hughes, S. Rees S, S. B. Kalindjian, and K. L. Philpott, “Principles of early drug discovery,” British journal of pharmacology, vol. 162, no. 6, pp. 1239-1249, 2011.
[13]. J. Guo, C. Yuan, M. Huang, et al, “Ganoderma lucidum derived polysaccharide enhances coix oil-based microemulsion on stability and lung cancer targeted therapy,” Drug Delivery, vol. 25, no. 1, pp. 1802–1810, 2018.
[14]. Viet Hung Tran, Phan Nguyen Truong Thang, Ha Minh Hien, et al. “Cytotoxic Activities and Fingerprint Analysis of Triterpenes by HPTLC Technique for Distinguishing Ganoderma Species from Vietnam and other Asian Countries,” Plants (Basel), vol. 11 no. 23, pp. 3397, 2022.

 Submit