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Determination of ethylene oxide and 2-chloro-ethanol using gas chromatography-tandem mass spectrometry: Internal standard and stainless-steel ball coupled with QuOil in high-fat food extraction

Dinh Viet Chien Nguyen Thi Hong Ngoc Bui Cao Tien Tran Cao Son Tran Trung Thanh Nguyen Ha Thanh Phung Cong Ly Pham Thi Thanh Ha Nguyen Thi Anh Huong Le Thi Hong Hao Thai Nguyen Hung Thu
Published 09/23/2022

Article Details

How to Cite
Dinh Viet Chien, Nguyen Thi Hong Ngoc, Bui Cao Tien, Tran Cao Son, Tran Trung Thanh, Nguyen Ha Thanh, Phung Cong Ly, Pham Thi Thanh Ha, Nguyen Thi Anh Huong, Le Thi Hong Hao, Thai Nguyen Hung Thu. "Determination of ethylene oxide and 2-chloro-ethanol using gas chromatography-tandem mass spectrometry: Internal standard and stainless-steel ball coupled with QuOil in high-fat food extraction". Vietnam Journal of Food Control. vol. 5, no. 4 (en), pp. 608-621, 2022
PP
608-621
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707

Main Article Content

Abstract

A determination method of ethylene oxide (ETO) and 2-chloro-ethanol (2-CE) in foods, especially those with high-fat content, by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed. The analysis was performed with a TG-WAX column (60 m × 0.32 mm × 1 µm), with a programmable temperature vaporization-large volume injection method (PTV-LVI). ETO, 2-CE, and isotope internal standard 2-chloroethanol-d4 (2-CE-d4) were extracted by the QuOil (a variation of QuEChERS method), in combination with the use of stainless-steel balls, especially effective with high-fat matrices and analyzed by gas chromatography-mass spectrometry (GC-MS/MS). For both compounds, the method has high specificity and selectivity with a detection limit of 0.003 mg/kg, the linear range of 0.01 - 0.2 mg/kg, reproducibility and recovery meet AOAC requirements. This method has been applied to analyze 1668 samples during the period 2021-2022 at the National Institute for Food Control (NIFC).

Keywords:

ethylene oxide, 2-chloro-ethanol, QuOil, stainless steel balls, GC-MS/MS.

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