Kombucha beverage is a traditional and popular natural fermented beverage consumed across the globe. Kombucha beverage is produced by fermenting sweetened black tea, sucrose with consortium of acetic acid bacteria and yeasts. It is important to understand the microbial composition in Kombucha to facilitate better control of the fermentation process. Therefore, this study characterized the dominant acetic acid bacteria in Kombucha sample. Acetic acid bacteria isolated from the Kombucha using glucose-ethanol medium. Based on morphological, biochemical characterization, and 16S rRNA gene, 14 isolated strains belong to 2 species and were identified as Komagataeibacter saccharivorans and Komagataeibacter rhaeticus. These strains showed good biofilm forming abilities. Furthermore, the antimicrobial activity against five pathogenic bacteria including Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Samonella enterica ATCC 13076, Pseudomonas aeruginosa ATCC 27853, Bacillus cereus ATCC 14579 of isolated acetic acid bacteria strains was also determined.
Acetic acid bacteria, Kombucha, antimicrobial activity, biofilm
. C. Dufresne, E. Farnworth, “Tea, Kombucha, and health: a review,” Food Research International, vol. 33, pp. 409-421, 2000.
. B. Wang, K. Rhtherfurd-Markwick, X. X. Zhang, A. N. Mutukumira, “Isolation and characterization of dominant acetic acid bacteria and yeast isolated from Kombucha samples at point of sale in New Zealand,” Current Research in Food Science, vol. 5, pp. 835-844, 2022.
. R. Jayabalan, R. V. Malbasa, E. S. Loncar, J. S. Vitas, M. Sathishkumar, “A Review on Kombucha tea-microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus,” Comprehensive Reviews in Food Science and Food Safety, vol. 13, pp. 538–550, 2014.
. Y. A. Ramirez Tapias, M. Victoria Di Monte, M. A. Peltzer, A. G. Salvay, “Bacterial cellulose films production by Kombucha symbiotic community cultured on different herbal infusions,” Food Chemistry, vol. 372, 131346, 2022.
. G. Subbiahdoss, S. Osmen, E. Reimhult, “Cellulosic biofilm formation of Komagataeibacter in Kombucha at oil-water interfaces,” Biofilm, vol. 4, 100071, 2022.
. Y. Yuan, F. Feng, L. Chen, Q. Yao, K. Chen, “Directional isolation of ethanol tolerant acetic acid bacteria from industrial fermented vinegar,” European Food Research and Technology, vol. 236, pp. 573-578, 2013.
. T. L. Yue, J. J. Pei, & Y. H. Yuan, “Purification and characterization of anti - Alicyclobacillus bacteriocin produced by Lactobacillus rhamnosus,” Journal of Food Protection, vo. 76, pp. 1575-1581, 2013.
. G. A. O’Toole, L. A. Pratt, P. I. Watnick, D. K. Newman, V. B. Weaver, R. Kolter, “Genetic approaches to study of biofilms,” Methods in Enzymology, vol. 310, pp. 91- 109, 1999.
. T. K. N. Nguyen, H. V. Duong, T. T. Nguyen, M. T. N. Dinh, M. H. Nguyen, “Characteristic of co-culture biofilm formed by Lactobacillus plantarum and Pediococcus acidilactici, and antagonistic effects of this biofilm on pathogen growth,” Japan Journal of Food Engineering, vol. 21, no. 2, pp. 81-87, 2020.
. C. Tu, W. Hu, S. Tang, L. Meng, Z. Huang, X. Xu, X. Xia, F. Azi, M. Dong, “Isolation and identification of Starmerella davenportii strain Do18 and its application in black tea beverage fermentation,” Food Science and Human Wellnes, vol. 9, pp. 355-362, 2020.