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High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants

Mac Thi Thanh Hoa Nguyen Ha Thanh Dang Thi Ngoc Lan Pham Thi Thanh Ha Vu Thi Thanh An Cao Cong Khanh
Received: 14 Mar 2022
Revised: 06 Jun 2022
Accepted: 06 Jun 2022
Published: 30 Jun 2022

Article Details

How to Cite
Mac Thi Thanh Hoa, Nguyen Ha Thanh, Dang Thi Ngoc Lan, Pham Thi Thanh Ha, Vu Thi Thanh An, Cao Cong Khanh. "High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants". Vietnam Journal of Food Control. vol. 5, no. 2 (en), pp. 160-169, 2022

Main Article Content


Glutaraldehyde (GA) is commonly used to disinfect surfaces and equipment in industries, agriculture, healthcare, laboratories, etc. To evaluate the quality of products containing GA, a HPLC method using 2,4-diphenylhydrazine derivative was developed. The analyte was separated by CN column with isocratic of acetonitrile and phosphoric acid 0,1% (ratio 50 : 50) and detected by PDA detector using a wavelength of 360 nm. The method was validated for specificity, linear curves, precision and accuracy which meet the requirements of AOAC.


Glutaraldehyde, glutaral, glutardehyde, aldehyde, DNPH, 2,4-diphenylhydrazine, disinfectant, HPLC.


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