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High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants

Mac Thi Thanh Hoa Nguyen Ha Thanh Dang Thi Ngoc Lan Pham Thi Thanh Ha Vu Thi Thanh An Cao Cong Khanh
Published 06/06/2022

Article Details

How to Cite
Mac Thi Thanh Hoa, Nguyen Ha Thanh, Dang Thi Ngoc Lan, Pham Thi Thanh Ha, Vu Thi Thanh An, Cao Cong Khanh. "High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants". Vietnam Journal of Food Control. vol. 5, no. 2 (en), pp. 160-169, 2022
PP
160-169
Counter
524

Main Article Content

Abstract

Glutaraldehyde (GA) is commonly used to disinfect surfaces and equipment in industries, agriculture, healthcare, laboratories, etc. To evaluate the quality of products containing GA, a HPLC method using 2,4-diphenylhydrazine derivative was developed. The analyte was separated by CN column with isocratic of acetonitrile and phosphoric acid 0,1% (ratio 50 : 50) and detected by PDA detector using a wavelength of 360 nm. The method was validated for specificity, linear curves, precision and accuracy which meet the requirements of AOAC.

Keywords:

Glutaraldehyde, glutaral, glutardehyde, aldehyde, DNPH, 2,4-diphenylhydrazine, disinfectant, HPLC.

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