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High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants

Mac Thi Thanh Hoa Nguyen Ha Thanh Dang Thi Ngoc Lan Pham Thi Thanh Ha Vu Thi Thanh An Cao Cong Khanh
Received: 14 Mar 2022
Revised: 06 Jun 2022
Accepted: 06 Jun 2022
Published: 30 Jun 2022

Article Details

How to Cite
Mac Thi Thanh Hoa, Nguyen Ha Thanh, Dang Thi Ngoc Lan, Pham Thi Thanh Ha, Vu Thi Thanh An, Cao Cong Khanh. "High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants". Vietnam Journal of Food Control. vol. 5, no. 2 (en), pp. 160-169, 2022
PP
160-169
Counter
578

Main Article Content

Abstract

Glutaraldehyde (GA) is commonly used to disinfect surfaces and equipment in industries, agriculture, healthcare, laboratories, etc. To evaluate the quality of products containing GA, a HPLC method using 2,4-diphenylhydrazine derivative was developed. The analyte was separated by CN column with isocratic of acetonitrile and phosphoric acid 0,1% (ratio 50 : 50) and detected by PDA detector using a wavelength of 360 nm. The method was validated for specificity, linear curves, precision and accuracy which meet the requirements of AOAC.

Keywords:

Glutaraldehyde, glutaral, glutardehyde, aldehyde, DNPH, 2,4-diphenylhydrazine, disinfectant, HPLC.

References

[1]. P. M. Borick, “Chemical sterilizers (chemosterilizers),” Advances in Applied Microbiology, vol. 10, pp. 291-312, 1968.
[2]. R. O. Beauchamp, Jr, M. B. St Clair, T. R. Fennell, D. O. Clarke, K. T. Morgan, F. W. Kari, “A critical review of the toxicology of glutaraldehyde”, Critical Reviews in Toxicology, vol. 22, no. 3-4, pp. 143-174. 1992.
[3]. A. F. Norman, J. S. Michael, “A simple method for determination of Glutaraldehyde,” The Journal of Histochemistry and Cytochemistry, vol. 17, no. 3, 1969.
[4]. Committee on Food Chemicals Codex, Food and Nutrition Board, et al., “Glutaraldehyde”, Food Chemicals Codex V, The National Academies Press, USA, 197-198, 2004.
[5]. United States Pharmacopeial Convention, “Glutaral disinfectant solution”, U.S. Pharmacopeia 40 - National Formulary 35, USA, 7678, 2017.
[6]. B. Pranaityt, A. Padarauskas, A. Dikčius, and R. Ragauskas, “Rapid capillary electrophoretic determination of glutaraldehyde in photographic developers using a cationic polymer coating”, Analytica Chimica Acta, vol. 507, no. 2, pp.185-190, 2004.
[7]. M. C. Meneta, D. Gueylardb, M-H. Fievetb, and A. Thuillierb, “Fast specific separation and sensitive quantification of bactericidal and sporicidal aldehydes by high-performance liquid chromatography: example of glutaraldehyde determination”, Journal of Chromatography B: Biomedical Sciences and Applications, vol. 692, no. 1, pp. 79-86, 1997.
[8]. B. P. Maggadani, M. Isfadhila, “High performance liquid chromatography analytical method validation for glutaraldehyde and benzalkonium chloride in disinfectants,” International Journal of Applied Pharmaceutics, vol. 10, no. 1, 2018.
[9]. J. W. Danielson, and R. D. Thompson, “Determination of glutaraldehyde and phenol in germicide products by capillary gas chromatography”, Journal of Chromatography A, vol. 724, no. 1, pp. 398-402. 1996.
[10]. G. Pieraccini, G. Bartolucci, M. Pacenti, S. Dugheri, P. Boccalon, and L. Focardi, “Gas chromatographic determination of glutaraldehyde in the workplace atmosphere after derivatization with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine on a solid-phase microextraction fibre”, Journal of Chromatography A, vol. 955, no. 1, pp. 117-124, 2002.
[11]. H. I. Kang, and H. S. Shin, “Sensitive determination of glutaraldehyde in environmental water by derivatization and gas chromatography-mass spectrometry”, Analytical Methods, no. 15, 2016.
[12]. United States Environmental Protection Agency, “Method 8315A - Determination of carbonyl compounds by high performance liquid chromatography (HPLC)”, Revision 1, 1996.

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