Glutaraldehyde (GA) is commonly used to disinfect surfaces and equipment in industries, agriculture, healthcare, laboratories, etc. To evaluate the quality of products containing GA, a HPLC method using 2,4-diphenylhydrazine derivative was developed. The analyte was separated by CN column with isocratic of acetonitrile and phosphoric acid 0,1% (ratio 50 : 50) and detected by PDA detector using a wavelength of 360 nm. The method was validated for specificity, linear curves, precision and accuracy which meet the requirements of AOAC.
Glutaraldehyde, glutaral, glutardehyde, aldehyde, DNPH, 2,4-diphenylhydrazine, disinfectant, HPLC.
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