Bifidobacterium is anerobic bacterium which is popular in human and animal intestine. B. infantis was successfully isolated from a new-born child’s fecal sample previously. In this study, the probiotic characteristics of this strain such as: extracellular enzyme production ability; antibiotic activity against food-born pathogen like E. coli, S. aureus; organic acid production and bile-salt sufferance were evaluated. The results showed that the isolated can produce amylase and protease enzyme, do not produce cellulase enzyme; the organic acids also were produced during development of bacterium. The bacterium can against E. coli and S. aureus and can grow in the media containing up to 1% of bile salt. Results of this study are the basis for further studies to apply these bacteria as a source of probiotic beneficial to human health in Vietnam.
Bifidobacterium infantis, probiotic, E. coli, exoenzymes, antibiotic activity.
. R. Fuller, "History and development of probiotics," Probiotics., pp 1-8, 1992.
. M. M. Invernici, S. L. Salvador , P. H. F. Silva , M. S. M. Soares, R. Casarin, D. B. Palioto, S. L. S. Souza, M. Taba Jr, A. B. Novaes Jr, F. A. C. Furlaneto, and Michel R Messora, "Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: a randomized clinical trial", Journal of Clinical Periodontology, vol. 45, no. 10, pp. 1198-1210, 2018.
. R L Kelley, D. Minikhiem, B. Kiely, L. O'Mahony, D. O'Sullivan, T. Boileau, and J. Soon Park, "Clinical benefits of probiotic canine-derived Bifidobacterium animalis strain AHC7 in dogs with acute idiopathic diarrhea", Veterinary Therapeutics:Reseearch in applied veterinary medcine, vol. 10, no.3, pp 121-130, 2009.
. A. M. O Leite , M. A. L. Miguel, R. S. Peixoto, P. Ruas-Madiedo , V. M. F. Paschoalin , B. Mayo, and S. Delgado, "Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains", Journal of Dairy Science, vol. 98, no. 6, pp 3622-3632, 2015.
. L. F. F. Oliveira, S. L Salvador, P. H. F. Silva, F. A. C. Furlaneto , L. Figueiredo, R. Casarin, E. Ervolino, D. B. Palioto, S. L. S. Souza, M. Taba. Jr , A. B. Novaes Jr , and M. R. Messora , "Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis", Journal of Periodontology, vol. 88, no. 2, pp 197-208, 2017.
. F. Turroni, C. Peano, D. A. Pass, E. Foroni, M. Severgnini, M. J. Claesson, C. Kerr, J. Hourihane, D. Murray, F. Fuligni, M. Gueimonde, A. Margolles, G. De Bellis, P. W O'Toole, D. van Sinderen, J. R. Marchesi, and M. Ventura, "Diversity of bifidobacteria within the infant gut microbiota", Plos One, vol. 7, no. 5, pp e36957, 2012.
. S Magaldi, S. Mata-Essayag, C. Hartung de Capriles, C. Perez, M. T. Colella, C. Olaizola, and Y. Ontiveros, “Well diffusion for antifungal susceptibility testing”, International Journal of Infectious Diseases, vol. 8, no. 1, pp 39-45, 2004.
. H. T. Nguyen, "Isolation and Selectation of bifidobacteria for application in probiotics production", Master of Science thesis dissertation, University of Science, Hanoi, 2017.
. A. Gómez Zavaglia, G. Kociubinski, P. Pérez, and G. De Antoni, “Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation”, Journal of Food Protection, vol. 61, no.7, pp. 865-873, 1998.
. G.-B. Kim and B. H. Lee, “Genetic analysis of a bile salt hydrolase in Bifidobacterium animalis subsp. lactis KL612”, Journal of Applied Microbiology, vol. 105, pp. 778-790, 2008.
. G. Larson, P. Falk, and L. C. Hoskins, “Degradation of Human Intestinal Glycosphingolipids by Extracellular Glycosidases from Mucin-degrading Bacteria of the Human Fecal Flora*”, The Journal of Biological Chemistry, vol. 263, no. 22, pp. 10790-10798,1988.
. M. Kitaoka, “Bifidobacterial Enzymes Involved in the Metabolism of Human Milk Oligosaccharides1–3,” Advances in Nutrition (American Society for Nutrition), vol. 3, no. 3. pp 422S–429S, 2012.
. E. J. Yun , S. Yu, N. J. Park, Y. Cho, N. R. Han, Y.-S.Jin, and K. H. Kim , “Metabolic and enzymatic elucidation of cooperative degradation of red seaweed agarose by two human gut bacteria”, Scientifc Reports, vol. 11, pp13955, 2021.
. D. J. Morrison and T. Preston, “Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism”, Gut Microbes, vol. 7, no. 3, pp. 189-200, 2016.
. G. Alessandri, M. C. Ossiprandi, J. MacSharry, D. van Sinderen, and M. Ventura, “Bifidobacterial Dialogue with Its Human Host and Consequent Modulation of the Immune System”, Frontiers in Immunology, vol. 10 (2348), pp. 1-12, 2019.
. F.Zuo. R. Yu, X. Feng, and L. Chen, "Characterization and in vitro properties of potential probiotic Bifidobacterium strains isolated from breast-fed infant feces", Annals of Microbiology, vol. 66, no. 3, pp1027-1037, 2016.
. M. Weng, K. Ganguli, W. Zhu, H. N..Shi, and W. A. Walker“Conditioned medium from Bifidobacteria infantis protects against Cronobacter sakazakii-induced intestinal inflammation in newborn mice”, American Journal of Physiology Gastrointestinal and Liver Physiology, vol. 306, no. 9, pp G779–G787, 2014.
. K. Ganguli, D. Meng, S. Rautava, L. Lu, W. A.Walker, and N. Nanthakumar "Probiotics prevent necrotizing enterocolitis by modulating enterocyte genes that regulate innate immune-mediated inflammation", American Journal of Physiology Gastrointestinal and Liver Physiology, vol. 304, no. 2, pp G132-G141, 2013.
. I. Aloisio, C. Santini, B. Biavati, G. Dinelli, A. Cencič, W. Chingwaru, L. Mogna, and D. Di Gioia, ‘’Characterization of Bifidobacterium spp. Strains for the treatment of enteric disorders in newborns’’, Applied Microbiology and Biotechnology, vol. 96, pp. 1561–1576, 2012.
. D. T. Men, P. T. Le, T. V. Tuan, N. T. T. Lan, N. T. M. Huyen, “Isolation and identification of Bifidobacterium spp. from infant intestinal tract”, Vietnamese Journal of Food Control, vol. 3, no. 2, pp. 125-132, 2020.
. E. Vamanu, A. Vamanu, and C. Gheorghe, “Isolation of a Lactobacillus plantarum strain used for obtaining a product for the preservation of fodders”, African Journal of Biotechnology, vol. 4, no. 5, pp. 403-408, 2005.
. A. M. O. Leite, M. A. L. Miguel, R. S. Peixoto, P. Ruas-Madiedo, V. M. F. Paschoalin, B. Mayo, and S. Delgado, “Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains”, Journal of Dairy Science, vol. 98, no. 6, pp 3622-3632, 2015.