Bìa tạp chí



Probiotic characteristics of Bifidobacterium infantis isolated in Vietnam

Bach Thi Nhu Quynh Ninh Thi Tuyet Lan Nguyen Thi Minh Huyen Do Thi Men Pham Thi Le Mai Thi Dam Linh
Published 06/28/2022

Article Details

How to Cite
Bach Thi Nhu Quynh, Ninh Thi Tuyet Lan, Nguyen Thi Minh Huyen, Do Thi Men, Pham Thi Le, Mai Thi Dam Linh. "Probiotic characteristics of Bifidobacterium infantis isolated in Vietnam". Vietnam Journal of Food Control. vol. 5, no. 2 (en), pp. 140-150, 2022

Main Article Content


Bifidobacterium is anerobic bacterium which is popular in human and animal intestine. B. infantis was successfully isolated from a new-born child’s fecal sample previously. In this study, the probiotic characteristics of this strain such as: extracellular enzyme production ability; antibiotic activity against food-born pathogen like E. coli, S. aureus; organic acid production and bile-salt sufferance were evaluated. The results showed that the isolated can produce amylase and protease enzyme, do not produce cellulase enzyme; the organic acids also were produced during development of bacterium. The bacterium can against E. coli and S. aureus and can grow in the media containing up to 1% of bile salt. Results of this study are the basis for further studies to apply these bacteria as a source of probiotic beneficial to human health in Vietnam.


Bifidobacterium infantis, probiotic, E. coli, exoenzymes, antibiotic activity.


[1]. R. Fuller, "History and development of probiotics," Probiotics., pp 1-8, 1992.
[2]. M. M. Invernici, S. L. Salvador , P. H. F. Silva , M. S. M. Soares, R. Casarin, D. B. Palioto, S. L. S. Souza, M. Taba Jr, A. B. Novaes Jr, F. A. C. Furlaneto, and Michel R Messora, "Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: a randomized clinical trial", Journal of Clinical Periodontology, vol. 45, no. 10, pp. 1198-1210, 2018.
[3]. R L Kelley, D. Minikhiem, B. Kiely, L. O'Mahony, D. O'Sullivan, T. Boileau, and J. Soon Park, "Clinical benefits of probiotic canine-derived Bifidobacterium animalis strain AHC7 in dogs with acute idiopathic diarrhea", Veterinary Therapeutics:Reseearch in applied veterinary medcine, vol. 10, no.3, pp 121-130, 2009.
[4]. A. M. O Leite , M. A. L. Miguel, R. S. Peixoto, P. Ruas-Madiedo , V. M. F. Paschoalin , B. Mayo, and S. Delgado, "Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains", Journal of Dairy Science, vol. 98, no. 6, pp 3622-3632, 2015.
[5]. L. F. F. Oliveira, S. L Salvador, P. H. F. Silva, F. A. C. Furlaneto , L. Figueiredo, R. Casarin, E. Ervolino, D. B. Palioto, S. L. S. Souza, M. Taba. Jr , A. B. Novaes Jr , and M. R. Messora , "Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis", Journal of Periodontology, vol. 88, no. 2, pp 197-208, 2017.
[6]. F. Turroni, C. Peano, D. A. Pass, E. Foroni, M. Severgnini, M. J. Claesson, C. Kerr, J. Hourihane, D. Murray, F. Fuligni, M. Gueimonde, A. Margolles, G. De Bellis, P. W O'Toole, D. van Sinderen, J. R. Marchesi, and M. Ventura, "Diversity of bifidobacteria within the infant gut microbiota", Plos One, vol. 7, no. 5, pp e36957, 2012.
[7]. S Magaldi, S. Mata-Essayag, C. Hartung de Capriles, C. Perez, M. T. Colella, C. Olaizola, and Y. Ontiveros, “Well diffusion for antifungal susceptibility testing”, International Journal of Infectious Diseases, vol. 8, no. 1, pp 39-45, 2004.
[8]. H. T. Nguyen, "Isolation and Selectation of bifidobacteria for application in probiotics production", Master of Science thesis dissertation, University of Science, Hanoi, 2017.
[9]. A. Gómez Zavaglia, G. Kociubinski, P. Pérez, and G. De Antoni, “Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation”, Journal of Food Protection, vol. 61, no.7, pp. 865-873, 1998.
[10]. G.-B. Kim and B. H. Lee, “Genetic analysis of a bile salt hydrolase in Bifidobacterium animalis subsp. lactis KL612”, Journal of Applied Microbiology, vol. 105, pp. 778-790, 2008.
[11]. G. Larson, P. Falk, and L. C. Hoskins, “Degradation of Human Intestinal Glycosphingolipids by Extracellular Glycosidases from Mucin-degrading Bacteria of the Human Fecal Flora*”, The Journal of Biological Chemistry, vol. 263, no. 22, pp. 10790-10798,1988.
[12]. M. Kitaoka, “Bifidobacterial Enzymes Involved in the Metabolism of Human Milk Oligosaccharides1–3,” Advances in Nutrition (American Society for Nutrition), vol. 3, no. 3. pp 422S–429S, 2012.
[13]. E. J. Yun , S. Yu, N. J. Park, Y. Cho, N. R. Han, Y.-S.Jin, and K. H. Kim , “Metabolic and enzymatic elucidation of cooperative degradation of red seaweed agarose by two human gut bacteria”, Scientifc Reports, vol. 11, pp13955, 2021.
[14]. D. J. Morrison and T. Preston, “Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism”, Gut Microbes, vol. 7, no. 3, pp. 189-200, 2016.
[15]. G. Alessandri, M. C. Ossiprandi, J. MacSharry, D. van Sinderen, and M. Ventura, “Bifidobacterial Dialogue with Its Human Host and Consequent Modulation of the Immune System”, Frontiers in Immunology, vol. 10 (2348), pp. 1-12, 2019.
[16]. F.Zuo. R. Yu, X. Feng, and L. Chen, "Characterization and in vitro properties of potential probiotic Bifidobacterium strains isolated from breast-fed infant feces", Annals of Microbiology, vol. 66, no. 3, pp1027-1037, 2016.
[17]. M. Weng, K. Ganguli, W. Zhu, H. N..Shi, and W. A. Walker“Conditioned medium from Bifidobacteria infantis protects against Cronobacter sakazakii-induced intestinal inflammation in newborn mice”, American Journal of Physiology Gastrointestinal and Liver Physiology, vol. 306, no. 9, pp G779–G787, 2014.
[18]. K. Ganguli, D. Meng, S. Rautava, L. Lu, W. A.Walker, and N. Nanthakumar "Probiotics prevent necrotizing enterocolitis by modulating enterocyte genes that regulate innate immune-mediated inflammation", American Journal of Physiology Gastrointestinal and Liver Physiology, vol. 304, no. 2, pp G132-G141, 2013.
[19]. I. Aloisio, C. Santini, B. Biavati, G. Dinelli, A. Cencič, W. Chingwaru, L. Mogna, and D. Di Gioia, ‘’Characterization of Bifidobacterium spp. Strains for the treatment of enteric disorders in newborns’’, Applied Microbiology and Biotechnology, vol. 96, pp. 1561–1576, 2012.
[20]. D. T. Men, P. T. Le, T. V. Tuan, N. T. T. Lan, N. T. M. Huyen, “Isolation and identification of Bifidobacterium spp. from infant intestinal tract”, Vietnamese Journal of Food Control, vol. 3, no. 2, pp. 125-132, 2020.
[21]. E. Vamanu, A. Vamanu, and C. Gheorghe, “Isolation of a Lactobacillus plantarum strain used for obtaining a product for the preservation of fodders”, African Journal of Biotechnology, vol. 4, no. 5, pp. 403-408, 2005.
[22]. A. M. O. Leite, M. A. L. Miguel, R. S. Peixoto, P. Ruas-Madiedo, V. M. F. Paschoalin, B. Mayo, and S. Delgado, “Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains”, Journal of Dairy Science, vol. 98, no. 6, pp 3622-3632, 2015.