Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
Nghiên cứu này nhằm sàng lọc các chủng vi khuẩn lactic có tiềm năng probiotic được phân lập từ phân su trẻ sơ sinh. Bằng phương pháp đánh giá khả năng: kháng vi khuẩn gây bệnh; kháng axit; kháng muối mật; kháng kháng sinh; tự kết dính đã chỉ ra hai chủng vi khuẩn lactic K24 và K36 thể hiện các đặc điểm probiotic tiềm năng. Chúng có khả năng kháng mạnh với các vi sinh vật gây bệnh Escherichia coli ATCC 25922, Salmonella enterica ATCC 13076, Bacillus cereus ATCC 21778, Vibrio cholerae ATCC 14033, Staphylococcus aureus ATCC 25923, Listeria monocytogenes ATCC 1911 với đường kính vòng dao động từ 16 – 27,5 mm; chịu axit trong dạ dày với tỷ lệ sống sót tại pH 2 sau 3 giờ lần lượt là 86,3% và 83,3%; chịu được muối mật 0,3% sau 3 giờ với tỷ lệ sống sót đạt 94,14% và 97,92%. Ngoài ra, chủng K24 và K36 có khả năng tự kết dính đạt 55,61%, 69,66% và có khả năng kháng với một số loại kháng sinh thường sử dụng ở người. Bằng phương pháp phân tích trình tự gen 16S rRNA đã xác định chủng K24 thuộc loài Lactobacillus plantarum và chủng K36 thuộc loài Lactobacillus acidophilus. Các chủng này cần được nghiên cứu sâu hơn nhằm đánh giá tính an toàn và hiệu quả của chúng như những ứng viên probiotic dùng cho người.
Vi khuẩn lactic, probiotic, phân su trẻ sơ sinh, Lactobacillus.
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