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Assessment of listeria monocytogenes, staphylococcus aureus and salmonella SPP. Contamination in dairy samples collected in Gia Lam and Ba Vi, Hanoi early 2019

Nguyen Thi Minh Huyen Tran Thi Hoa Ninh Thi Tuyet Lan Tran Thi Hien
Published 11/30/2019

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How to Cite
Nguyen Thi Minh Huyen, Tran Thi Hoa, Ninh Thi Tuyet Lan, Tran Thi Hien. "Assessment of listeria monocytogenes, staphylococcus aureus and salmonella SPP. Contamination in dairy samples collected in Gia Lam and Ba Vi, Hanoi early 2019". Vietnam Journal of Food Control. vol. 2, no. 4, pp. 22-32, 2019

Main Article Content


Milk and dairy products from dairy farms around Hanoi greatly contribute to the consumed milk quantity in Hanoi. The use of fresh milk or pasteurized milk becomes more and more popular in the daily life of local people. Milk and dairy products were widely sold in numerous stores, particularly in Xuan Mai, Ba Vi, Phu Dong and Gia Lam. However, there have not yet been any studies to assess the pathogenic bacterial contamination of these products. In our study, 49 samples including 23 raw milk samples, 12 pasteurized milk samples, and 14 yogurt samples were collected in order to examine the presence of food­born pathogenic bacteria such as Listeria monocytogenes, Staphylococcus aureus, Salmonella spp. using PCR method. This fast and accurate method works based on the specific amplification of tested bacterial DNA. The results showed that one of the samples may contain Staphylococcus aureus while another may be contaminated with Listeria monocytogenes. None of the samples was contaminated with Salmonella spp. The results were confirmed by gene sequencing.


Bacteria, food­poisoning, milk, bacterial contamination, Hanoi


1. Năm 2018 ­ Những nỗ lực của ngành Sữa Việt Nam (15/1/2019). Hiệp hội Sữa Việt Nam.
2. Otto Garcia et. al. (2006), “The Economics of Milk Production in Hanoi, Vietnam with Particular Emphasis on Small­scale Producers”, FAO, PPLPI Working Paper No.33.
3. Nguyen Anh Phong, (2015), “Small holder involvement in Vinamilk supply chain, Vietnam”, FAO
4. Mahendra Pal et. al. (2016), “Bacterial Contamination of Dairy Products”, Beverage & Food World, 9 (43), 40 ­ 43.
5. Edward M. Fox et. al. (2017), “Editorial: Microbial Food Safety along the Dairy Chain”, Frontiers in Microbiology, (8), 1612.
6. Jin­Qiang Chen et. al. (2017), “PCR­based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources”, Food Science and Human Wellness, (6), 39 ­ 59.
7. Konstantia Halatsi et. Al, “PCR detection of Salmonella spp. using primers targeting the quorum sensing gene sdiA”, FEMS Microbiol Lett 259 (2006), 201 ­ 207.
8. Joseph Odumeru. (2002), “Current Microbial Concerns in the Dairy Industry”, Food Safety magazine.
9. Odd G. Brakstad et. al. (1992), “Detection of Staphylococcus aureus by Polymerase Chain Reaction Amplification of the nuc Gene”, Journal of Clinical Microbiology, 7 (30), 1654 ­ 1660.
10. Thúy An (2017). Hậu Giang: Trẻ ngộ độc do uống sữa có vi khuẩn Staphylococcus aureus. Quân đội Nhân dân online 06/11/2017.
11. Minh Nhật (2018). Cảnh báo về “thủ phạm” gây ra các vụ ngộ độc tập thể. daibieunhandan.vn online 16/12/2018
12. Yi Wang et. al. (2014), “Rapid and sensitive detection of Listeria monocytogenes by cross­priming amplification of lmo0733 gene”, FEMS Microbiol Lett, 361, 43 ­ 51.
13. Eliezer Avila Gandra et. al. (2016), “Detection by multiplex PCR of Staphylococcus aureus, S. intermedius and S. hyicus in artifcially contaminated milk”, Ciência Rural, Santa Maria, v.46, n.8, 1418­1423
14. KLM Moganedi et. al. (2007), “Optimisation of the PCR­invA primers for the detection of Salmonella in drinking and surface waters following a pre­cultivation step”, Water SA, Vol. 33 No. 2.
15. M. Radhika et. al., “A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species”, Journal of Microbiology Online, ISSN 1678 ­ 4405
16. Kumar S., Balakrishna K. & Batra H. (2006), “Detection of Salmonella enterica serovar Typhi (S. Typhi) by selective amplification of invA, viaB, fliC- d and prt genes by polymerase chain reaction in mutiplex format”, Letters in Applied Microbiology, 2 (42), 149 ­ 154.