Raw foods such as fish and meat sold at local markets are easily pathogenically infected because retailers do not keep them in proper food preservation equipment. In addition, food packaging using single-use plastic grocery bags is a burden for waste management and aggravates the “white pollution”. In this research, we created biodegradable bacterial cellulose membrane (BCm) loaded with raw bacteriocins that were collected from isolated lactic acid bacteria (LAB) and test their ability to preserveraw fish and meat.
The 2mm thick and 94% wet BC membrane was obtained from the surface fermentation of Acetobacterxylinum in the medium containing 50% of coconut water and 15% sucrose after 03 days. The isolated LAB from sour cabbage, kimchi and yogurt were microscopically observed, biochemically characterized and species identified by mass spectrometry. The raw bacteriocin from Lactobacillus plantarum showing the most antimicrobial capability among the isolates could inhibit the growth of 3 tested strains of Escherichia coli, Staphylococcus aureus and Bacillus subtilis at the antimicrobial activity of 100 AU/ml by agar-well diffusion method. BCm treated with the bacteriocins of 100 AU/ml in 45 minutes was proved to be able to well preserve fish for 24 hours and pork for 18 hours at ambient temperatures, following Vietnam Standard for catfish fillet (TCVN 8338:2010) and for meat (TCVN 7046:2009).
Bacteriocin, bacterial cellulose, biodegradation, food preservation
1. Azeredo, H.M.C., Barud, H., Farinas, C.S., Vasconcellos, V.M., Claro, A.M.(2019), “Bacterial cellulose as a raw material for food and food packaging applications”, Frontiers in Sustainable Food Systems, 3(7).
2. Cotter, P.D., Hill, C., Ross, R.P. (2005), “Bacteriocins: developing innate immunity for food”, Nature Reviews Microbiology, 3(10), 777-88.
3. Da Silva Sabo, S., Vitolo, M., Gonzalez, J.M.D., Oliveira, R.P.S. (2014), “Overview of Lac tobacillus plantarum as a promising bacteriocin producer among lactic acid bacteria”, Food Research International, 64, 527-536.
4. Dos Santos, C.A., dos Santos, G.R., Soeiro, V.S., dos Santos, J.R., Rebelo, M.d.A., Chaud, M.V., Gerenutti, M., Grotto, D., Pandit, R., Rai, M., Jozala, A.F.(2018), “Bacterial nanocel lulose membranes combined with nisin: a strategy to prevent microbial growth”, Cellulose, 25(11), 6681-6689.
5. De Vuyst, L., and Leroy, F. (2007),“Bacteriocins from lactic acid bacteria: production, purification,and food applications”, Journal of Molecular Microbiology and Biotechnology, 13(4), 194-199.
6. Kormin, S., Rusul G., Radu, S., Ling, F.H. (2001), “Bacteriocin-producing lactic acid bacteria isolated from traditional fermented food”, The Malaysian journal of medical sciences: MJMS, 8(1), 63-68.
7. Joint annual health review (JAHR) (2015), Ministry of Health of Vietnam, Hanoi.
8. Nguyen T.H., Tran T.T.A. (2008), “Immobilizing Lactococcus lactic cell and applicating bacterial cellulose membrane treated with bacteriocin to preserving pork”, Science & Technology Development, 11(9), 777-88.
9. Ogunbanwo, S.T., Sanni, A.I., and Onilude A.A (2003), “Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1”, African Journal of Biotechnology, 2(8), 219-227.
10. Onda, T., et al. (2002),“Widespread distribution of the bacteriocin-producing lactic acid cocci in Miso-pasteproducts”, Journalof applied microbiology, 92(4), 695-705.
11. Tagg, J.R., and McGiven A. R. (1971), “Assay system for bacteriocins”, Applied microbiol ogy, 21(5), 943.
12. Todorov, S.D., Dicks, L.M.T. (2005), “Lactobacillus plantarum isolated from molasses produces bacteriocins active against Gram-negative bacteria”. Enzyme and Microbial Technology, 36(2-3) 318-326.
13. Vignolo, Graciela M., et al. (1995), “Influence of growth conditions on the production of lactocin 705, a bacteriocin produced by Lactobacillus casei CRL705”, Journal of Applied Bacteriology, 78(1) 5-10.