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Study on the hydrolysis process of food and feed samples using high pressure asher (HPA-S) to determine several amino acids by high performance liquid chromatography

Published 05/27/2019

Article Details

How to Cite
Dinh Viet Chien, Nguyen Tien Luyen, Nguyen Thi Hong Ngoc, Pham Cong Hieu, Do Tat Thanh, Pham Thu Giang, To Quoc Tuong, Nguyen Thi Lan, Duong Minh Tuan, Doan Van Kien. "Study on the hydrolysis process of food and feed samples using high pressure asher (HPA-S) to determine several amino acids by high performance liquid chromatography". Vietnam Journal of Food Control. vol. 2, no. 2 (en), pp. 44-50, 2019
PP
44-50
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212

Main Article Content

Abstract

The study researchs on the hydrolysis process of food and feed samples using High Pressure Asher (HPA-S) to determine several amino acids by high-performance liquid chromatography (HPLC). HPA-S equipment is mainly used for samples treatment in analysis of metals by spectroscopy. However, the study also found that HPA-S can be used in hydrolysis of food and feed samples in order to analyze amino acids. The temperature of HPA-S equipment could reach 300oC and maintain continuously at 130 bar pressure, completely digetsing the most complex samples matrix within an hour. The successful study of the application of HPA-S to hydrolyze samples in order to analyze amino acids makes the time of sample preparation significantly shorter but still gave the equivalent stability, even higher than the common samples hydrolyzation.

Keywords:

food, feeding, amino acids, HPA-S, hydrolysis, HPLC

References

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