The study researchs on the hydrolysis process of food and feed samples using High Pressure Asher (HPA-S) to determine several amino acids by high-performance liquid chromatography (HPLC). HPA-S equipment is mainly used for samples treatment in analysis of metals by spectroscopy. However, the study also found that HPA-S can be used in hydrolysis of food and feed samples in order to analyze amino acids. The temperature of HPA-S equipment could reach 300oC and maintain continuously at 130 bar pressure, completely digetsing the most complex samples matrix within an hour. The successful study of the application of HPA-S to hydrolyze samples in order to analyze amino acids makes the time of sample preparation significantly shorter but still gave the equivalent stability, even higher than the common samples hydrolyzation.
food, feeding, amino acids, HPA-S, hydrolysis, HPLC
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