Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
This study aimed to identify potential probiotic lactic acid bacteria (LAB) strains isolated from meconium. Based on evaluations of antimicrobial activity, acid and bile salt tolerance, antibiotic resistance, and auto aggregation ability, two strains (K24 and K36) were identified as promising probiotic candidates. The strains exhibited strong inhibitory activity against several pathogenic bacteria, including Escherichia coli ATCC 25922, Salmonella enterica ATCC 13076, Bacillus cereus ATCC 21778, Vibrio cholerae ATCC 14033, Staphylococcus aureus ATCC 25923, and Listeria monocytogenes ATCC 1911. Inhibition zones ranging from 16 to 27.5 mm. At pH 2 (3 h), survival rates were 86.3% (K24) and 83.3% (K36). In 0.3% bile salts, survival rates were 94.14% (K24) and 97.92% (K36) after 3 h. Furthermore, strains K24 and K36 demonstrated strong auto-aggregation, with percentages of 55.61% and 69.66%, respectively. Both strains also showed resistance to several commonly used antibiotics. Based on 16S rRNA gene sequence analysis, strain K24 was identified as Lactobacillus plantarum, while strain K36 was identified as Lactobacillus acidophilus. These strains warrant further investigation to evaluate their safety and efficacy as probiotic candidates for human use.
LAB, probiotics, meconium, Lactobacillus.
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