Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
The optimization of the chlorophyll extraction process from Vietnamese galangal (Alpinia spp.) leaves was investigated using Response Surface Methodology (RSM). The synergistic effects of three independent variables, including extraction time, extraction temperature, and solid-to-solvent ratio, were evaluated through a Central Composite Design (CCD). Analysis of variance (ANOVA) revealed that the solid-to-solvent ratio had the most significant impact on the extraction yield, whereas the interactions between variables were not statistically significant (p > 0.05). The optimal extraction conditions were determined at a temperature of 57.5℃ for 45 min with a liquid-to-solid ratio of 50:1 (mL/g), yielding a chlorophyll content of 235.56 ± 2.17 mg/100 g. The experimental results demonstrated high agreement with the predicted model (> 97%). Furthermore, the application of this method to analyze 13 real samples suggests that galangal leaves are a promising source for natural chlorophyll extraction.
Galangal leaves, chlorophyll, extraction, optimization, Response Surface Methodology (RSM).
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