Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
Mold contamination and mycotoxin production remain major food safety concerns in Vietnam due to climatic vulnerability. With limited access to modern preservation technologies, essential oils (EOs) offer a promising natural strategy for mold control, which aligns with consumer preferences for natural preservatives. This study aimed to evaluate the chemical composition and antifungal activity of Vietnamese balm (Elsholtzia ciliata) EO, and to directly compare these properties with those of citral, its major component. Citral, a monoterpene composed of neral and geranial, is recognized as Generally Recognized as Safe (GRAS). Analysis of E. ciliata EO from Hong Van, Hanoi, showed high concentrations of citral derivatives (47.12%). Both E. ciliata EO and citral inhibited Aspergillus niger growth by suppressing mycelial development and delaying sporulation. E. ciliata EO displayed a minimum inhibitory concentration (MIC) of 0.34 mg/mL and a minimum fungicidal concentration (MFC) of 5.38 mg/mL, whereas citral's MIC and MFC were lower (0.18 mg/mL and 1.39 mg/mL, respectively), indicating greater potency. Thus, citral alone exhibited stronger antifungal activity than the whole EO, highlighting E. ciliata as a valuable natural source for citral extraction and antifungal application.
antifungal activity, citral, Elsholtzia ciliata, essential oil, Aspergillus niger.
[1]. S. Aiswarya and N. S. Delna, "Prevalence of spoilage and mycotoxigenic fungi in commonly consumed foods and produce: A laboratory-based study," Library of Sciences, vol. 1, no. 1, 2026.
[2]. L. H. Nguyen and T. C. Tran, "Studies on the Aspergillus fr.: fri. of Fructus mali collected from the traditional medicine stores in Hanoi," Vietnam Journal of Community Medicine, vol. 65, special issue 5, pp. 222-227, 2024 (in Vietnamese).
[3]. T. T. H. Vo, T. T. Nguyen, T. M. N. Nguyen, and T. M. D. Nguyen, "Survival and growth of food borne harmful fungi during storage of Vietnamese ramie leaf rice cake," Journal of Dairy and Food Research, vol. 45, issue 1, pp. 135-140, 2024.
[4]. E. P. Petrović, K. Vrandečić, J. Cosić, T. Siber, and S. Godena, "Antifungal efficacy of essential oils and their predominant components against olive fungal pathogens," Agriculture, vol. 15, issue 3, 2025.
[5]. N. Q. Chi et al., "Chemotypic diversity, antimicrobial activities, and molecular docking analysis of essential oils from four elsholtzia species in Northern Vietnam," Crops, vol. 6, no. 1, p. 2, 2025.
[6]. M. Kashiri, "Effect of citral incorporation on the antimicrobial, barrier, mechanical, and UV-protective properties of pectin-beeswax films for food packaging applications," Applied Food Research, vol. 5, no. 2, 2025.
[7]. N. Karakaplan, E. Goz, E. Tosun, and M. Yuceer, "Optimization of hydrodistillation of essential oil from Mentha spicata L. by using response surface methodology," Latin American Applied Research, vol. 53, no. 3, pp. 203-213, 2023.
[8]. H. Wang et al., "Antifungal evaluation of plant essential oils and their major components against toxigenic fungi," Industrial Crops and Products, vol. 120, pp. 180-186, 2018.
[9]. L. Yu, Y. Zhou, Y. Chen, Y. Wang, Q. Gu, and D. Song, "Antifungal activity and mechanism of Litsea cubeba (Lour.) Persoon essential oil against the waxberry spoilage fungi Penicillium oxalicum and its potential application," International Journal of Food Microbiology, vol. 411, p. 110512, 2024.
[10]. S. Burt, "Essential oils: their antibacterial properties and potential applications in foods - a review," International Journal of Food Microbiology, vol. 94, no. 3, pp. 223-253, 2004.
[11]. L. Un Khasanah, S. Ariviani, E. Purwanto, and D. Praseptiangga, "Chemical composition and citral content of essential oil of lemongrass (Cymbopogon citratus (DC.) Stapf) leaf waste prepared with various production methods," Journal of Agriculture and Food Research, vol. 19, Mar. 2025.
[12]. F. A. Sheelmarevaa et al., "Composition and medicinal applications of Citrus essential oils: Current insights and future perspectives," Phytomedicine Plus, vol. 19, pp. 101570, 2025.
[13]. Y. R. Choi, M. K. Park, A. S. Lee, Y. S. Kim, and M. J. Kim, "Citral, a major component of lemon myrtle (Backhousia citriodora) essential oil, inhibits adipogenesis in 3T3-L1 preadipocytes during mitotic clonal expansion," Italian Journal of Food Science, vol. 37, no. 1, pp. 220-233, 2025.
[14]. O. O. Fatunmibi, O. T. Asekun, I. S. Njoku, and J. O. Ogah, "Chemical composition, antioxidant and antimicrobial Activity of the essential oil from the leaves of Cymbopogon citratus," American Journal of Essential Oils and Natural Products, vol. 11, no. 1, pp. 1-5, 2023.
[15]. M. M. Gutiérrez-Pacheco et al., "Mechanisms and applications of citral’s antimicrobial properties in food preservation and pharmaceuticals formulations," Antibiotics, vol. 12, no. 11, 2023.
[16]. T. Q. A. Tran and T. T. H. Vo, "Study on the chemical composition and antibacterial properties of Vietnamese balm essential oil (Elsholtzia ciliata)," Journal of Agricultural Science and Technology of the University of Agriculture and Forestry, vol. 6, no. 1, pp. 2881-2890, 2022 (in Vietnamese).
[17]. D. D. Nguyen et al., "Chemical composition of the essential oils of Elsholtzia ciliata (Thunb.) Hyl. grown in Phu Tho province," Journal of Science and Technology, vol. 27, no. 2, pp. 80-84, 2022 (in Vietnamese).
[18]. J. Y. Liang, J. Xu, Y. Y. Yang, Y. Z. Shao, F. Zhou, and J. L. Wang, "Toxicity and synergistic effect of elsholtzia ciliata essential oil and its main components against the adult and larval stages of Tribolium castaneum," Foods, vol. 9, no. 3, p. 345, 2020.
[19]. J. Ju, Y. Lei, Y. Guo, H. Yu, Y. Cheng, and W. Yao, "Eugenol and citral kills Aspergillus niger through the tricarboxylic acid cycle and its application in food preservation," LWT, vol. 173, 2023.
[20]. J. Glamočlija, M. Soković, V. Tešević, G. A. Linde, and N. B. Colauto, "Chemical characterization of Lippia alba essential oil: an alternative to control green molds," Brazilian Journal of Microbiology, vol. 42, no. 4, p. 1537, 2011.