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Development of qualitative methods of bifidobacterium bifidum in probiotic with Real-time PCR method

Pham Nhu Trong Le Thanh Long Nguyen Thanh Trung Ta Thi Yen Pham Thi Loan Nguyen Thi Xuan Huong
Received: 08 Feb 2018
Revised: 22 Mar 2018
Accepted: 30 Mar 2018
Published: 30 Mar 2018

Article Details

How to Cite
Pham Nhu Trong, Le Thanh Long, Nguyen Thanh Trung, Ta Thi Yen, Pham Thi Loan, Nguyen Thi Xuan Huong. "Development of qualitative methods of bifidobacterium bifidum in probiotic with Real-time PCR method". Vietnam Journal of Food Control. vol. 1, no. 1, pp. 13-17, 2018

Main Article Content


Bifidobacterium strains with probiotic effects have been widely used in dairy products, food additives and pharmaceuticals. Especially, Bifidobacterium bifidum (B. bifidum) is usually presented into food products such as functional food. However, it is difficult to detect, and quantify B. bifidum in a sample with a combination of different probiotics. In Vietnam, there is no official standard method to identify and quantify B. bifidum in the sample with the mix of probiotic species. To fulfil the requirements of a robust quality management, we have developed a quantitative real-time PCR assay based on groEL gene for accurate identification and quantification of Bifidobacterium bifidum. The developed assay allows an unambiguous speciesspecific detection. We built the real-time PCR method to detect and identify B. bifidum in functional and supplemented food with specific up to 100% and reproducibility (SR<0.25) suitable with Annex F AOAC: 2016. This real-time PCR method is rapidly and effectively than conventional method. It takes only 24 hours to detect and identify B. bifidum in compare with at least a period of 3-5 days for conventional methods. The low quantitative limit is 105 CFU/g/mL, which is consistent with probiotic and powdered milk products with a declared quality of more than 106 CFU/g/mL.


Bifidobacterium bifidum, real-time PCR, probiotics


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