Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
Steroid hormones and endocrine compounds play an important role in regulating body physiology, however, they can pose potential risk to environmental and human health. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously analyze eight steroid hormones (i.e., boldenone, boldenone-17-alpha, progesterone, testosterone, melengestrol acetate, zeranol, trenbolone acetate, trenbolone-17-alpha ) in meat and meat products. Samples were extracted using the QuEChERS method with acetonitrile (ACN) as solvent and and clean-up procedure using PSA and C18 sorbent combined with MgSO₄. The conditions for hormone separation on the LC-MS/MS system included a C18 reversed-phase column (2.1 × 150 mm, 3.5 µm) with a mobile phase of 0.1% formic acid and 10 mM ammonium formate in water and ACN using gradient elution and flow rate 0.5 mL/min. The analytical method had good specificity with a linear range (R2 > 0.99) from 1-10 ng/mL, recovery of the substances reached 70.1 - 115%, relative standard deviation (RSD%) in the range of 1.23 - 12.1%. The limit of quantification of all eight substances was 1.0 µg/kg. The method was applied to determine 8 hormones in 20 meat and meat product samples in Hanoi. The analytical results showed that none of the 8 steroid hormone analytes were detected in all samples.
Soluble solid, sensory quality, polyscias fruticosa, flavonoid, tea bag.
[1]. Department of Pharmaceuticals, “Research methods of medicinal materials in Vietnamese,” Ho Chi Minh City Medicine and Pharmacy University, 2014.
[2]. Lai Thi Ngoc Ha, Vu Thi Thu, “Oxidative stress and natural antioxidants in Vietnamese,” Journal of Science and Development, vol.7, no.5, pp. 667-677, 2019.
[3]. P. Karak, “Biological activitive of flavonoids: An overview,” International Journal of Pharmaceutical Sciences and Research, vol. 10, no. 4, pp. 1567-1574, 2019.
[4]. S. Kumar and A.K. Pandey, “Chemistry and biological activities of flavonoids: an overview,” Scientific World Journal, pp. 1-16, 2013.
[5]. A. Miller, “Antioxidant flavonoids: structure, function and clinical usage,” Alternative Medicine Review: a Journal of Clinical Therapeutic, vol.1, no. 2, pp. 103-111, 1995.
[6]. A. Hanneken ,F. F. Lin, J. Johnson, P. Maher, “Flavonoids protect human retinal pigment epithelial cells from oxidative-stress-induced death,” Investigative Ophthalmology & Visual, vol. 47, no. 7, pp. 3164-77, 2006.
[7]. B. M. Bernard, N. Pakianathan, M. C. Divakar, “On the antipyretic, antiinflammatory, analgesic, and molluscicidal properties of Polyscias fruticosa (L.) Harms,” Ancient Science of Life, vol. 17, no. 4, pp. 313 – 319, 1998.
[8]. Do Tat Loi, “Vietnamese medicinal plants and herbs in Vietnamese,” Science and Technics Publishing House, 2013 [in Vietnamese].
[9]. Nguyen Thi Thu Huong, Luong Kim Bich, “Research on the anti-depressant and stress effects of Dinh Lang,” Journal of Medicinal Materials, vol. 6, pp. 84 – 86, 2001 [in Vietnamese].
[10]. Ho Luong Nhat Vinh, “Researched chemical composition. Inhibition effect of α-amylase and α-glucosidase enzymes of polyscias fruticosa (L.) Harms leaf extract in Vietnamese,” Hanoi University of Pharmacy, 2014 [in Vietnamese].
[11]. Vietnam Ministry of Health, “Vietnamese Pharmacopoeia IV,” 2009 [in Vietnamse].
[12]. Vo Van Chi, “Dictionary of Vietnamese medicinal plants, volume 1 in Vietnamese,” Medical Publishing House, 1977 [in Vietnamese].
[13]. Dang Thu Tam, “Study on the production process of instant tea from Polyscias fruticosa (Dinh Lang),” Thai Nguyen University of Agriculture and Forestry, 2016 [in Vietnamese].
[14]. C. Chang, M. Yang, H. Wen and J. Chem, “Estimation of flavonoid total content in propolis by two complementary colorimetric methods,” Journal of Food and Drug Analysis, 10(7), pp. 178-182, 2002.
[15]. [15] AOAC, “Official Methods of Analysis of AOAC International, 20th Ed.,” AOAC International, Gaithersburg, Maryland, USA, 2016.
[16]. [16] AOAC International, “Official Method 983.17: Solids (Total) in fruit and vegetable juices. In Official Methods of Analysis of AOAC International (22th ed.),” Gaithersburg, MD: AOAC International, 2023.
[17]. Vietnamese standard TCVN 3215:1979 on Food Products - Sensory analysis and scoring method. Science and Technology.
[18]. Vietnamese standard TCVN 7975:2008 on Herbal Tea in Tea Bags. Science and Technology.
[19]. Le Bach Tuyet, “Basic technological processes in food production in Vietnamese,” Education Publishing House, 1996 [in Vietnamese].
[20]. T. Ayhan, D. Cüneyt, T. Mehmet, T, İsmail, “Physicochemical properties of Turkish green tea powder: effects of shooting period, shading, and clone,” Turkish Journal of Agriculture and Forestry, vol. 38, no. 2; pp. 233 -241, 2014.
[21]. T. Katsube, Y. Tsurunaga, M. Sugiyama, T. Furuno and Y. Yamasaki, “Effect of air-drying temperature on antioxidant capacity and stability of polyphenolic compounds in mulberry (Morus alba L.) leaves,” Food Chemistry, vol. 113, no. 4, pp. 964-969, 2009.
[22]. A. S. Deve, T. S. Kumar, K. Kumaresan and V. S. Rapheal, “Extraction process optimization of polyphenols from Indian Citrus sinensis – as novel antiglycative agents in the management of diabetes mellitus,” Journal of Diabetes and Metabolic Disorders, vol. 13, no. 1, pp. 1-10, 2014.
[23]. Man L.V, Dat L.Q, Hien N.T, Nguyet T.L.M, Ha T.T.T, Food processing technology in Vietnamese, Ho Chi Minh City National University Publishing House, 2011.
[24]. Nguyen Thi My Trang, Vu Ngoc Boi and Dang Xuan Cuong, “Research on the effects of some technological factors on the quality of Asparagus oficinalis (Asparagus oficinalis) tea bags in Vietnamese,” Science Magazine Aquatic technology, vol, 2, pp. 66 – 70, 2015 [in Vietnamese].
[25]. Vu Kim Dung, Hoang Thi Hien, Pham Thanh Trung, Nguyen Viet Phuong, “Research on the effects of some technological factors on the quality of Morindan officianalis tea bags in Vietnamese,” Journal of Aquaculture Science, vol. 2, pp. 3 – 10, 2019 [in Vietnamese].
[26]. Phan Thi Dieu Hien, “Research on anthocyanin extraction from purple sweet potatoes and its applications,” Da Nang University of Education in Vietnamese, 2017 [in Vietnamese].