Bìa tạp chí

 

009bet

Simultaneous quantitative analysis of some main components in cinnamon leaves (Cinnamomum cassia) by HPLC-PDA

Bui Thi Lan Phuong Le Bao Tram Quach Huy Hoang Tran Van On Nguyen Thi Kieu Anh
Received: 30 Jun 2024
Revised: 18 Sep 2024
Accepted: 22 Sep 2024
Published: 30 Sep 2024

Article Details

How to Cite
Bui Thi Lan Phuong, Le Bao Tram, Quach Huy Hoang, Tran Van On, Nguyen Thi Kieu Anh. "Simultaneous quantitative analysis of some main components in cinnamon leaves (Cinnamomum cassia) by HPLC-PDA". Vietnam Journal of Food Control. vol. 7, no. 3, pp. 506-517, 2024
PP
506-517
Counter
57

Main Article Content

Abstract

Cinnamon leaves, a part of the cinnamon tree, are used as raw materials to extract cinnamon essential oil and as a spice in food processing. Trans-cinnamaldehyde is the main ingredients that have biological effects and high concentration in cinnamon, cinnamic acid is often used as a marker in the quantification methods of cinnamon, coumarin is the ingredient that needs to be controlled due to risk of liver toxicity. So that, cinnamaldehyde (CAL), cinnamic acid (CA) and coumarin (CM) have been choosen to determin quality of cinnamon leaves. The analytes in the test sample were extracted with methanol:water ratio 9:1 by ultrasound twice 30 minutes and resting for 30 minutes between extractions. The ultrasonic extract was analyzed using an HPLC-PDA, C18 column (250×4.6 mm, 5 µm) at 25°C, mobile phase system consisting of acetonitrile and 0.05% phosphoric acid is eluted according to the program's gradient, detection wavelength 280 nm. The method was determined according to AOAC 2016 guidelines with appropriate specificity and linearity ranging from 51.90-691.31 µg/mL for coumarin, 1.00-20.10 µg/mL for cinnamic acid and 52.45-609.8 µg/mL for trans-cinnamaldehyde, good repeatability with RSD from 0.46% to 0.61%, accuracy of coumarin, cinnamic acid and cinnamaldehyd were 101.0%, 100.7% and 100.7% respectively, meets AOAC requirements at 3 concentration levels. The study applied quantitative research methods to quantify coumarin, cinnamic acid and trans-cinnamaldehyde on 17 C. cassia leaves samples collected in provinces in Vietnam. In result, analyte concentrations varied widely between samples, with coumarin ranging from 0.53- 2.47%, cinnamic acid from 0.01-0.08%, and cinnamaldehyde from 0.95-2.56%.

Keywords:

Cinnamon leaves, cinnamaldehyde, coumarin, HPLC-PDA, simultaneous quantification

References

[1]. Vo Van Chi, Dictionary of Vietnamese medicinal plants, Medical Publishing House, 2012 (in Vietnamese).
[2]. Ministry of Health, Vietnam Pharmacopoeia V, 2017 (in Vietnamese).
[3]. Anna Vallverdú-Queralt, Jorge Regueiro, Miriam Martínez-Huélamo, José Fernando Rinaldi Alvarenga, Leonel Neto Leal, and R. M. Lamuela-Raventos, "A comprehensive study on the phenolic profile of widely used culinary herbs and spices: rosemary, thyme, oregano, cinnamon, cumin and bay," Food Chemistry, vol. 154, pp. 299-307, 2013.
[4]. Putri Wulandari and Elsa Yuniarti, "Bioactivity Potential and Chemical Compounds of Cinnamomum: Literature Review," Jurnal Penelitian Pendidikan IPA, vol. 9, no. 5, pp. 1-7, 2023.
[5]. Rui Wang, Ruijiang Wang, and Bao Yang, "Extraction of essential oils from five cinnamon leaves and identification of their volatile compound compositions," Innovative Food Science and Emerging Technologies, vol. 10, pp. 289-292, 2009.
[6]. The Korea Food and Drug Administration, The Korea Pharmacopoiea 12th (KP12).
[7]. The Government of the Hong Kong Special Administrative Region of the People’s Republic of China, Hong Kong Chinese Materia Medica Standards, 2009.
[8]. Chinese Pharmacopoeia Commission, Pharmacopoeia of the people's republic of china, 2020.
[9]. United State Pharmacopoeia, USP. 2022.
[10]. European Pharmacopoeia, EP 11. 2023.
[11]. Daniel Brancheau, Brijesh Patel, and Marcel Zughaib, "Do Cinnamon Supplements Cause Acute Hepatitis?," American Journal Case Reports, vol. 16, pp. 250-254, 2005.
[12]. Bui Thi Lan Phuong et al., "Simultaneous quantitative analysis of coumarin, cinnamic acid and cinnamaldehyde in cinnamon bark towards standardization of cinnamon medicinal herbs," Journal of Medicinal Materials, vol. 29, no. 4, pp. 222-228, 2024 (in Vietnamese).
[13]. AOAC INTERNATIONAL, "Guidelines for Standard Method Performance Requirements, Appendix F.," 2016.

 Submit