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Simultaneous determination of nicotinamide mononucleotide, niacinamide, and nicotinic acid in health supplements using liquid chromatography method

Vu Thi Nhat Le Vu Thi Trang Hasara Savindi Rupasinghe Nguyen Quang Huy Tran Thi Bich Thao
Received: 01 Nov 2023
Revised: 16 Nov 2023
Accepted: 16 Nov 2023
Published: 29 Dec 2023

Article Details

How to Cite
Vu Thi Nhat Le, Vu Thi Trang, Hasara Savindi Rupasinghe, Nguyen Quang Huy, Tran Thi Bich Thao. "Simultaneous determination of nicotinamide mononucleotide, niacinamide, and nicotinic acid in health supplements using liquid chromatography method". Vietnam Journal of Food Control. vol. 6, no. 4 (en), pp. 393-404, 2023

Main Article Content


This study aimed to develop and validate an HPLC-PDA method for simultaneous quantification of three compounds: nicotinamide mononucleotide (NMN), nicotinic acid, and niacinamide in dietary supplements. The sample preparation procedure was simple, fast, and used environmentally green extracted solvents. The HPLC conditions were as follows: C18 column (250 mm x 4.6 mm, 5 µm); mobile phase: 10 mM phosphate buffer pH = 3: methanol (90:10, v/v), UV detection at wavelength 261 nm. The method met the AOAC criteria for validation, including selectivity, linearity (R2> 0.999 for all analytes in the range of 0.1-50.0 µg/mL), sensitivity (LOD: 0.4-0.45 mg/kg for NMN, 0.1-0.61 mg/kg for nicotinic acid, and 0.1-0.6 mg/kg for niacinamide; LOQ: 1.3-1.5 mg/kg for NMN, 0.49-2.00 mg/kg for nicotinic acid, and 0.47-1.90 mg/kg for niacinamide), accuracy (recovery: 95.5-103.0 % for NMN, 92.5-104.1 % for nicotinic acid, and 95.3-103.8 % for niacinamide), precision (RSDr: 0.54-3.79 % for NMN, 0.39-4.82 % for nicotinic acid, and 0.81-5.14% for niacinamide; RSDR: 1.03-3.70 % for NMN, 1.75-4.10 % for nicotinic acid, and 1.83-4.48 % for niacinamide). The method was successfully applied to analyze 20 supplement products collected in Hanoi.


Nicotinamide mononucleotide, NMN, niacinamide, nicotinic acid, HPLC-PDA


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