This study aimed to develop and validate an HPLC-PDA method for simultaneous quantification of three compounds: nicotinamide mononucleotide (NMN), nicotinic acid, and niacinamide in dietary supplements. The sample preparation procedure was simple, fast, and used environmentally green extracted solvents. The HPLC conditions were as follows: C18 column (250 mm x 4.6 mm, 5 µm); mobile phase: 10 mM phosphate buffer pH = 3: methanol (90:10, v/v), UV detection at wavelength 261 nm. The method met the AOAC criteria for validation, including selectivity, linearity (R2> 0.999 for all analytes in the range of 0.1-50.0 µg/mL), sensitivity (LOD: 0.4-0.45 mg/kg for NMN, 0.1-0.61 mg/kg for nicotinic acid, and 0.1-0.6 mg/kg for niacinamide; LOQ: 1.3-1.5 mg/kg for NMN, 0.49-2.00 mg/kg for nicotinic acid, and 0.47-1.90 mg/kg for niacinamide), accuracy (recovery: 95.5-103.0 % for NMN, 92.5-104.1 % for nicotinic acid, and 95.3-103.8 % for niacinamide), precision (RSDr: 0.54-3.79 % for NMN, 0.39-4.82 % for nicotinic acid, and 0.81-5.14% for niacinamide; RSDR: 1.03-3.70 % for NMN, 1.75-4.10 % for nicotinic acid, and 1.83-4.48 % for niacinamide). The method was successfully applied to analyze 20 supplement products collected in Hanoi.
Nicotinamide mononucleotide, NMN, niacinamide, nicotinic acid, HPLC-PDA
[1]. A.C.Lyons, J. E. Grable and S. H. Joo, "A cross-country analysis of population aging and financial security", The Journal of the Economics of Ageing, no. 12, pp. 96-117, 2018.
[2]. "Recent research into nicotinamide mononucleotide and aging" [Online]. Available: https://www.nature.com/articles/d42473-022-00002-7. [Accessed 3 8 2023].
[3]. H. Nadeeshani, J. Li, T. Ying, B. Zhang, J. Lu, "Nicotinamide mononucleotide (NMN) as an anti-aging health product –Promises and safety concerns", Journal of Advanced Research, no. 37, pp. 267-278, 2022.
[4]. B. J. Weiss, "Nicotinamide Mononucleotide (NMN): Official Info Source", 29/11/2022. [Online]. Available: https://www.nmn.com/news/fda-bans-labeling-nmn-as-a-supplement. [Accessed 3 8 2023].
[5]. K. I. Martin, D. A. Glaser, "Cosmeceuticals: The New Medicine of Beauty", The Journal of the Missouri State Medical Association, no. 108(1), pp. 60-63, 2011.
[6]. J. Çat and M. Yaman, "Determination of nicotinic acid and nicotinamide forms of vitamin B3 (niacin) in fruits and vegetables by HPLC using postcolumn derivatization system", Pakistan Journal of Nutrition, pp. 563-570, 2019.
[7]. F. M. Carpi, M. Cortese, G. Orsomando, et al, "A new and highly sensitive LC-MS/MS method for simultaneous quantification of NMN and related pyridine compounds in different mouse tissues", Medicine, Biology, Chemistry, 2016.
[8]. K. M. Niu, T. Bao, L. Gao, et al, "The Impacts of Short-Term NMN Supplementation on Serum Metabolism, Fecal Microbiota, and Telomere Lenghth in Pre-Aging Phase", Frontiers in Nutrition, no. 8, 2021.
[9]. T. Yamane, M. Imai, T. Bamba and S. Uchiyama, ''Nicotinamide mononucleotide (NMN) intake increases plasma NMN and insulin levels in healthy subjects", Clinical Nutrition ESPEN, pp. 83-86, 2023.
[10]. Le Thi Thuy, Vu Thi Trang, Tran Hoang Giang, Tran Mai Van và Le Thi Hong Hao, " Simultaneous determination some of B group vitamins in nutritional products by liquid chromatography tandem mass spectrometry following enzymatic hydrolysis", Vietnam Journal of Food Control, vol 2, no. 1, pp. 38-44, 2019. (In Vietnamese)
[11]. G. R. M. S. Ibrahim, "LC-MS/MS based analysis of vitamin B3 and B6 in healthy and obese Emirati population", United Arab Emirates University, 2018.
[12]. Cuu My Cuc, Vo Thi Bach Hue, Nguyen Thi Ngoc Han, "Developing a simultaneously quantify six vitamin: B1, B2, b3, B6, B9 and B12 by UPLC-MS/MS", Journal of Pharmacy, vol. 57, no. 2, 2017. (In Vietnamese)
[13]. S. McClure, "Simultaneous determination of total vitamins B1, B2, B3, and B6 in infant formula and related nutritionals by enzymatic digestion and LC-MS/MS—a multi-laboratory testing study final action: AOAC method 2015.14", Journal of AOAC International, vol. 4, no. 103, pp. 1060-1072, 2020.
[14]. L. Formentini, F. Moroni and A. Chiarugi, "Detection and pharmacological modulation of nicotinamide mononucleotide (NMN) in vitro and in vivo.", Biochemical Pharmacology, vol. 10, no. 77, pp. 1612-1620, 2009.
[15]. TCVN 9045:2012 (EN 15652:2009). Foodstuffs -Determination of niacin by high performance liquid chromatography (HPLC). (In Vietnamese)
[16]. Chromadex, TRU NIAGEN, "Quantitative analysis of twenty-two NMN Consumer Products". [Online]. Available: https://s23.q4cdn.com/937095816/files/doc_downloads/2021/Quantitative-Analysis-of-22-NMN-Consumer-Products-Oct-2021.pdf. [Accessed 30 7 2023].