Cholesterol is a soft, pale yellow steroid fat that exists in the body's cell membranes and is transported in the plasma of most animals. This compound plays an important role in the physiology of the human body. However, excess cholesterol in the diet is also one of the leading causes of cardiovascular diseases. Therefore, the quantification of cholesterol in foods and functional foods is essential so that consumers can choose a diet containing the right amount of cholesterol. This study used gas chromatography-mass spectrometry to quantify cholesterol, with ISQ 7000 GC-MS system, TraceGOLD TG-5MS chromatographic column, and electron impact (EI) ionization mode. The validity of the method was confirmed according to the guidelines of the Association of Official Analytical Collaborations (AOAC). The results showed that the method has good specificity, the linearity range from 5 to 100 μg/mL in working solution, the detection limit of 1.5 to 15 mg/kg, the quantification limit of 5.0 to 50 mg/kg, depending on the matrix; the method's recovery were from 92.6 to 108.4% and the precision has relative standard deviations of 1.18 - 2.84%, meeting the requirements of AOAC. The method was used to analyze samples of dairy foods and functional foods randomly purchased in the market.
cholesterol, GC-MS, dairy-based foods, functional foods.
[1]. Sekhar, M. Sonal, et al., "Physiological role of cholesterol in human body." Dietary sugar, salt and fat in human health, pp. 453-481, 2020.
[2]. Tabas, Ira, Kevin Jon Williams, and Jan Borén, "Subendothelial lipoprotein retention as the initiating process in atherosclerosis: update and therapeutic implications." Circulation, vol. 116(16), pp. 1832-1844, 2007.
[3]. Vietnam National Heart Association, “Recommendations on diagnosis and treatment of lipid disorders”, 2015.
[4]. Fletouris, D. J., et al., "Rapid determination of cholesterol in milk and milk products by direct saponification and capillary gas chromatography." Journal of Dairy Science, vol. 81(11), pp. 2833-2840, 1998.
[5]. Naeemi, Esmaeel D., et al., "Rapid and simple method for determination of cholesterol in processed food." Journal of AOAC international, vol. 78(6), pp. 1522-1524, 1995.
[6]. Chen, Yan-Zong, et al., "Determination of cholesterol and four phytosterols in foods without derivatization by gas chromatography-tandem mass spectrometry." Journal of food and drug analysis, vol. 23(4), pp. 636-644, 2015.
[7]. National technical standards committee TCVN/TC/F13 Analytical and sampling methods, TCVN 12385:2018, Directorate for Standards, Metrology and Quality, Ministry of Science and Technology, Hanoi, 2018.
[8]. AOAC Official Methods of Analysis: Guidelines for Standard Method Performance Requirements, 2016.
[9]. Commission Implementing Regulation (EU) 2021/808 on the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results as well as on the methods to be used for sampling and repealing Decisions 2002/657/EC and 98/179/EC.
[10]. Oh, H. I., T. S. Shin, and E. J. Chang. "Determination of cholesterol in milk and dairy products by high-performance liquid chromatography." Asian-Australasian Journal of Animal Sciences, vol. 14(10), pp. 1465-1469, 2001.
[11]. Ramalho, Heryka Myrna M., Susana Casal, and M. Beatriz Prior Pinto Oliveira. "Total cholesterol and desmosterol contents in raw, UHT, infant formula powder and human milks determined by a new fast micro-HPLC method." Food Analytical Methods, vol. 4, pp. 424-430, 2011.