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Simultaneous determination of benzyldimethyloctylammonium chloride and benzyldimethyldodecylammonium chloride by CE-C4D

Tran Thi My Hao Dinh Viet Chien Vo Thi Minh Anh Hoang Quoc Anh Kieu Van Anh Pham Thi Quynh Dinh Son Luong Pham Hoang Nguyen Tran Hoang Minh Anh Cao Cong Khanh Nguyen Van Khoa Le Thi Hong Hao Nguyen Thi Anh Huong
Received: 27 Apr 2023
Revised: 28 May 2023
Accepted: 28 May 2023
Published: 29 Sep 2023

Article Details

How to Cite
Tran Thi My Hao, Dinh Viet Chien, Vo Thi Minh Anh, Hoang Quoc Anh, Kieu Van Anh, Pham Thi Quynh, Dinh Son Luong, Pham Hoang Nguyen, Tran Hoang Minh Anh, Cao Cong Khanh, Nguyen Van Khoa, Le Thi Hong Hao, Nguyen Thi Anh Huong. "Simultaneous determination of benzyldimethyloctylammonium chloride and benzyldimethyldodecylammonium chloride by CE-C4D". Vietnam Journal of Food Control. vol. 6, no. 3, pp. 246-258, 2023

Main Article Content


Benzyldimethyloctylammonium chloride (BAC-C8) and Benzyldimethyldodecyl ammonium chloride (BAC-C12) are bactericidal surfactants which widely used in the pharmaceutical industry as anti-microbial disinfectants in skin antiseptic solution. According to current international regulations, the standard of finished antiseptic solution must have regulations on the limit of preservatives when used in the formulation. In this study, simultaneous determination of BAC-C8 and BAC-C12 was performed by capillary electrophoresis using capacitively coupled contactless conductivity detection (CE-C4D) with analytical conditions including: fused silica capillary (50 µm ID.) with effective length of 40 cm, background electrolyte solution of 100 mM acetic acid (Ace) added 30% acetonitril (ACN); hydrodynamic sample injection at height of 10 cm in 20 s; separation voltage of +15 kV. The detection limit of BAC-C8 and BAC-C12 were 1.5 mg/L and 3.5 mg/L, respectively. This method has been applied to determine the content of BAC-C8 and BACC12 in the disinfectant solution samples.


BAC-C8, BAC-C12, disinfectant, CE-C4D


[1]. A. P. Golin, D. Choi, and A. Ghahary, “Hand sanitizers: A review of ingredients, mechanisms of action, modes of delivery, and efficacy against coronaviruses,” American Journal of Infection Control, vol. 48, no. 9, pp. 1062-1067, 2020.
[2]. M. A. Liebert, “Final Report on the Safety Assessment of Benzalkonium Chloride,” Journal of the American College of Toxicology, vol. 8, no. 4, 1989.
[3]. European Parliament, Council of the European Union. Biocidal products regulation. European Union, Brussels, Belgium, 2012.
[4]. European Commission. Commission implementing decision (EU) 2016/1950 of 4 November 2016 on the non-approval of certain biocidal active substances pursuant to regulation (EU) no. 528/2012 of the European Parliament and of the Council. European Commission, Brussels, Belgium, 2016.
[5]. Food and Drug Administration, “Safety and effectiveness of consumer antiseptics; topical antimicrobial drug products for over-the-counter human use,” Fed Regist, vol. 81, pp. 61106-61130, 2016.
[6]. Food and Drug Administration, “Safety and effectiveness of health care antiseptics; topical antimicrobial drug products for over-the-counter human use,” Fed Regist, vol. 82, pp. 60474-60503, 2017.
[7]. Food and Drug Administration, “Safety and effectiveness of consumer antiseptics; topical antimicrobial drug products for over-the-counter human use; proposed amendment of the tentative final monograph; reopening of administrative record,” U.S. Silver Spring, MD, 2016.
[8]. L. E. Maier, H. P. Lampel, T. Bhutani, and S. E. Jacob, “Hand Dermatitis: A Focus on Allergic Contact Dermatitis to Biocides,” Dermatologic Clinics, vol. 27, no. 3, pp. 251-264, 2009.
[9]. U.S. Environmental Protection Agency (EPA), “Reregistration eligibility decision for alkyl dimethyl benzyl ammonium chloride (ADBAC),” Washington, DC, 2006.
[10]. Kuča, K., Bielavská, M., Cabal, J., & Dohnal, V, “Determination of benzalkonium bromide homologues in disinfection products using high‐performance liquid chromatography,” Analytical letters, vol. 38, no. 4, pp. 673-682, 2005.
[11]. T. Bertuzzi, and A. Pietri, “Determination of Benzalkonium Homologues and Didecyldimethylammonium in Powdered and Liquid Milk for Infants by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry,” Food Analytical, vol. 7, pp. 1278-1284, 2014.
[12]. Y. Xian, H. Dong, Y. Wu, X. Guo, X. Hou, and B. Wang, “QuEChERS-based purification method coupled to ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC- MS/MS) to determine six quaternary ammonium compounds (QACs) in dairy products,” Food Chemistry, vol. 212, pp. 96-103, 2016.
[13]. V. G. Amelin, and D. Bolshakov, “Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry,” Zavodskaya Laboratroiya. Diagnostika Materialov vol. 86, no. 8, pp. 23-31, 2020.
[14]. Le Thi Hong Hao, Pham Thi Ngoc Mai, Nguyen Thi Anh Huong, Nguyen Van Anh, Pham Tien Duc, Vu Thi Trang, Application of electrophoresis in food analysis. Hanoi: Science and Technology Publishing House, 2016.
[15]. N. T. A. Huong, P. T. N. Mai, D. T. Tuoi, T. T. Thao, J. Sáiz, N. T. H. Quynh, P. C. Hauser, M. T. Duc, “Simple semi-automated portable capillary electrophoresis instrument with contactless conductivity detection for the determination of β-agonists in pharmaceutical and pig-feed samples,” Journal of Chromatography A, vol. 1360, pp. 305-311, 2014.
[16]. AOAC Official Methods of Analysis, Appendix F: Guidelines for standard method performance requirements, 2012.
[17]. P. Luan, “Theory basis of high performance capillary electrophoresis chromatography,” Textbook for students majoring in Analytical Chemistry, Hanoi University of Sciences, Hanoi, 2005.
[18]. L. Ching-Erh, C. Wen-Cheng, and L. Wei-Chen. "Capillary zone electrophoretic separation of alkylbenzyl quaternary ammonium compounds: effect of organic modifier," Journal of Chromatography A, vol. 722, no. 1-2, pp. 345-352, 1996.
[19]. C. S. Weiss, J. S. Hazlett, M. H. Datta, and M. H. Danzer, “Determination of quaternary ammonium compounds by capillary electrophoresis using direct and indirect UV detection,” Journal of Chromatography A, vol. 608, pp. 325-332, 1992.
[20]. E. Piera, P. Erra, M. R. Infante, “Analysis of cationic surfactants by capillary electrophoresis,” Journal of Chromatography A, vol. 757, pp. 275-280, 1997.