Bìa tạp chí

 

009bet

Study on the determination of some diamine in urine by LC-MS/MS

Tong Thi Ngan Mai Ngọc Thanh Nguyen Thi Hien Pham Thi Quyen Nguyen Phuc Dan Nguyen Thi Huyen Nguyen Thi Minh Hoa Vu Tung Lam Chu Thi Thu Hien Hoang Thi Lan Anh Nguyen Thi Anh Huong
Published 12/26/2022

Article Details

How to Cite
Tong Thi Ngan, Mai Ngọc Thanh, Nguyen Thi Hien, Pham Thi Quyen, Nguyen Phuc Dan, Nguyen Thi Huyen, Nguyen Thi Minh Hoa, Vu Tung Lam, Chu Thi Thu Hien, Hoang Thi Lan Anh, Nguyen Thi Anh Huong. "Study on the determination of some diamine in urine by LC-MS/MS". Vietnam Journal of Food Control. vol. 6, no. 1, pp. 66-82, 2022
PP
66-82
Counter
327

Main Article Content

Abstract

Isocyanate is a common name for chemical compounds containing one or more - NCO groups. There have been many studies showing that isocyanates pose a risk to human health when exposed and can cause occupational poisoning. When absorbed into the body, the isocyanates will be converted into the corresponding amines. Therefore, the determination of these diamines in urine will contribute to the assessment of exposure to isocyanates. This article presents a study on simultaneous determination of 4,4′-methylenedianiline (MDA), hexamethylene diamine (HDA), isophoron diamine (IPDA) in urine by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method detection limit (MDL) were 0.074, 0.059, and 0.053 ng/mL for HDA, MDA, IPDA, respectively. The method quantitation limit (MQL) were 0.243, 0.194 and 0.177 ng/mL for HDA, MDA, IPDA, respectively. The method's recovery ranged from 86.38 to 105.3% with the repeatability RSDr < 6%. The method was successfully applied to simultaneously determine the content of HDA, MDA and IPDA in 30 urine samples of workers at auto repair garages in Bac Ninh province. The results showed that HDA, MDA and IPDA were detected in these samples with different concentrations.

Keywords:

LC-MS/MS, diamine, MDA, HDA, IPDA, urine.

References

[1]. Aalto-Korte, K., et al, “Occupational contact allergy to monomeric isocyanates,” Contact Dermatitis, vol. 67, no. 2, pp. 78-88, 2012.
[2]. Parker, John E., Petsonk, E. Lee, and Weber, Susan L, “Hypersensitivity pneumonitis and organic dust toxic syndrome,” Immunology and Allergy Clinics of North America, vol. 12, no. 2, pp. 279-290, 1992.
[3]. Redlich, C. A. and Karol, M. H, “Diisocyanate asthma: clinical aspects and immunopathogenesis,” International Immunopharmacol, vol. 2, no. 2, pp. 213-224, 2002.
[4]. International Labour Office, List of Ocupational Disease, Occupational Safety and Health Series, no.74, 2010.
[5]. Ministry of Health, “Decision No. 27/2006/QD-BYT on adding 04 occupational diseases to the list of insured occupational diseases,” 2006 (in Vietnamese).
[6]. M. Mirmohammadi, M. Ibrahim, and G. Saraji, “Evaluation of Hexamethylene Diisocyanate as an Indoor Air Pollutant and Biological Assessment of Hexamethylene Diamine in the Polyurethane Factories,” pp. 81-98, 2011.
[7]. A. Maitre, M. Berode, A. Perdrix, M. Stoklov, J. M. Mallion, H. Savolainen, “Urinary hexane diamine as an indicator of occupational exposure to hexamethylene diisocyanate,” International Archives Occupational and Environmental Health, vol. 69, no.1, pp. 65-68, 1996.
[8]. L. M. Fabbri, D. Danieli, S. Crescioli, P. Bevilacqua, S. Meli, M. Saetta, and C. E. Mapp, “Fatal asthma in a subject sensitized to toluene diisocyanate,” The American Review of Respiratory Disease, vol. 137, no. 6, pp. 1494-1498, 1988.
[9]. L. G. T. Gaines, K. W. Fent, S. L. Flack, J. M. Thomasen, L. M. Ball, D. B. Richardson, K. Ding, S. G. Whittaker, and L. A. Nylander-French, “Urine 1,6-hexamethylene diamine (HDA) levels among workers exposed to 1,6-hexamethylene diisocyanate (HDI),” The Annals of Occupation Hygiene, vol. 54, no.6, pp. 678-691, 2010.
[10]. H. Tinnerberg, G. Skarping, M. Dalene, and L. Hagmar “Test chamber exposure of humans to 1,6-hexamethylene diisocyanate and isophorone diisocyanate,” International Archives Occupational and Environmental Health, vol. 67, no. 6, pp. 367-374, 1995.
[11]. H. Wikman, P. Piirilä, C. Rosenberg, R. Luukkonen, K. Kääriä, H. Nordman, H. Norppa, H. Vainio, and A. Hirvonen, “N-Acetyltransferase genotypes as modifiers of diisocyanate exposure-associated asthma risk,” Pharmacogenetics, vol. 12, no. 3, pp. 227-233, 2002.
[12]. American Conference of Governmental Industrial Hygienists, “2016 TLVs and BEIs: Based on the documentation of the threshold limit values for chemical substances and physical agents & biological exposure indices,” American Conference of Governmental Industrial Hygienists Cincinnati, OH , 2016.
[13]. M. Lépine, M. Sleno, J. Segage, and S. Gangé, “A validated LC/MS/MS method for 4,4’‐methylenedianiline quantitation in human urine as a measure of 4,4’‐methylene diphenyl diisocyanate exposure,” Rapid Communications in Mass Spectrometry, vol. 33, pp. 600-606, 2019.
[14]. M. Lépine, M. Sleno, J. Segage, and S. Gangé, “A validated UPLC-MS/MS method for the determination of aliphatic and aromatic isocyanate exposure in human urine,” Analytical and Bioanalytical Chemistry, vol. 412, no. 3, pp. 753-762, 2019.
[15]. T. D. Li, F. Liu, X. F. Pan, X. Tao, W. Zhao, and H. F. Yan, “Determination of methylenedianiline in urine by high performance liquid chromatography-tandemmass spectrometry,” Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi (Chinese Medical Association Publishing House Ltd.), vol. 36, no. 4, pp. 308-311, 2018.
[16]. D. Bhandari, B. A. Bowman, A. B. Patel, D. M. Chambers, V. R. De Jesús, and B. C. Blount , “UPLC-ESI-MS/MS method for the quantitative measurement of aliphatic diamines, trimethylamine N-oxide, and β-methylamino-l-alanine in human urine,” Journal of Chromatograpy. B, Analytival Technologies in the Biomedical and Life Sciences, vol. 1083, pp. 86-92, 2018.
[17]. A. Marand, D. Karlsson, M. Dalene, and G. Skarping, “Determination of amines as pentafluoropropionic acid anhydride derivatives in biological samples using liquid chromatography and tandem mass spectrometry,” Analyst, vol. 129, no. 6, pp. 522-528, 2004.
[18]. S. L. Flack, L. M. Ball, and L. A. Nylander-French, “Occupational exposure to HDI: progress and challenges in biomarker analysis,” Journal of Chromatograpy. B, Analytival Technologies in the Biomedical and Life Sciences, vol. 878, no. 27, pp. 2635-2642, 2010.
[19]. H. Harari, D. Bello, S. Woskie, and C. Redlich, “Development of an Interception Glove Sampler for Skin Exposures to Aromatic Isocyanates,” The Annals of Occupational Hygiene, vol. 60, no. 9, pp. 1092-1103, 2016.
[20]. AOAC Official Methods of Analysis, Appendix F: Guidelines for standard method performance requirements, 2012.
[21]. Council implementing regulation (EU) 2021/804, Official Journal of the European Union, vol. 64, pp. 96, 2021.

 Submit