Bìa tạp chí


Development of the process for extracting and isolating hesperidin from Chenbi to establish as a reference standard

Published 03/30/2022

Article Details

How to Cite
Vu Thi Thanh An, Cao Cong Khanh, Mac Thi Thanh Hoa, Tran Hung Son, Do Thi Thanh Thuy, Nguyen Thi Huyen. "Development of the process for extracting and isolating hesperidin from Chenbi to establish as a reference standard". Vietnam Journal of Food Control. vol. 5, no. 1, pp. 68-76, 2022

Main Article Content


Hesperidin is the main active ingredient in the flavonoid group, accounting for about 3 % of Chenbi. According to studies, hesperidin has anti-oxidant, analgesic, and anti-inflammatory effects on the nervous system as well as enhancing the body's resistance. Chenbi is a simple medicine, easy to obtain, and uses medicinal material for the potential extraction and isolation of Hesperin with high yield. The study developed and validated the process of determining hesperidin on medicinal samples and raw materials by HPLC. The study was successful in making the active ingredient hesperidin with a purity > 95 % (HPLC) sufficient as a condition to be used as a referenced material. Extraction and isolation process using 70 % ethanol extraction solvent with extraction method is reflux heating then cleaning with activated carbon and purified on chromatographic equipment to obtain high recovery efficiency, simple and economical process.


Hesridin, extraction, isolation, Chenbi.


[1]. N. M. Duc, Preparation and establishment of standard substances from nature to serve the research, testing, and standardization of medicinal herbs and traditional medicines", Center for Science and Technology Communication of Ho Chi Minh City, Ho Chi Minh, 2013.
[2]. K. Jeongtae, W. Myung-Bok, A. Meejung, A. Tanaka, H. Matsuda, and Taekyun Shin, “Benefits of hesperidin in central nervous system disorders: a review,” Anatomy & Cell Biology, 2019.
[3]. A. Roohbakhsh, H. Parhiz, F. Soltani, R. Rezaee and, M. Iranshahi, “Molecular mechanisms behind the biological effects of hesperidin and hesperetin for the prevention of cancer and cardiovascular diseases,” Life Sciences, 2014.
[4]. A. Garg, S. Garg, L. J. D. Zaneveld, and A. K. Singla, “Chemistry and Pharmacology of The Citrus Bioflavonoid Hesperidin,” Phytother, Res. 15, pp. 655-669, 2001.
[5]. M. Szymański, D. Młynarek, A. Szymański and, I. Matławska, “Simultaneous Determination of Diosmin and Hesperidin in Pharmaceuticals by RPLC using Ionic Liquids as Mobile Phase Modifiers,” Iranian Journal of Pharmaceutical Research, vol. 15, no. 1, pp. 141-148, 2016.
[6]. A. N. Adham, “Qualitative and Quantitative Estimation of Hesperidin in Peel and Juice of Citrus Fruits by RP-HPLC Method Growing in Kurdistan Region/Iraq,” International Journal of Pharmaceutical Sciences Review and Research, vol. 33, no 2, pp. 220-224, 2015.
[7]. G. Dugo and A. Di Giacomo, “A. Citrus: The genus Citrus”, Taylor & Francis: London, UK, 1st ed.; 2002; pp. 169-170.