Strawberries are economically important owing to their unique flavour, health benefits, and nutritional aspects. Decay after harvest is a major issue in the development of strawberry industry. Currently, the most effective method for controlling decay after harvest is storage of strawberries at low temperature combined with usage of chemical fungicides. However, long-term usage of chemical fungicides not only causes pathogen resistance but also is harmful for human health and environment. Biocontrol method for the management of disease after strawberry harvest has great practical significance. In this study, totally 200 bacterial strains isolated from the surface of healthy strawberry fruit were tested for antagonistic activity against five fungal pathogens. Among them, VK199 strain was potent against all five postharvest spoilage fungi on strawberry fruit and then utilized for further study. This strain was identified as Bacillus siamensis based on sequence analysis of 16S rDNA. The extracellular lytic enzymes, including cellulase, amylase, protease and chitinase released by B. siamensis VK199 were detected. Furthemore, in vivo, the results of biological control efficacy test showed that B. siamensis VK199 suppressed the occurrence of diseases caused by Botrytis cinerea, Colletotrichum gloeosporioides, Mucor nidicola and Rhizopus delemar of strawberry during storage.
Antagonistic bacterium, antifugal activity, biological control, strawberry.
. A. S. Dukare, S. Paul, V. E Nambi, R. K. Gupta, R. Singh, K. Sharma, and R. K. Vishwakarma, “Exploitation of microbial antagonists for the control of postharvest diseases of fruits: a review,” Critical Reviews in Food Science and Nutrition, vol. 59, no. 9, pp. 1-16, 2018.
. F. Wang, J. Xiao, Y. Zhang, R. Li, L. Liu, and J. Deng, “Biocontrol ability and action mechanism of Bacillus halotolerans against Botrytis cinerea causing grey mould in postharvest strawberry fruit,” Postharvest Biology and Technology, vol. 174, 111456, 2021.
. W. Q. Ye, Y. F. Sun, Y. J. Tang, and W. W. Zhou, “Biocontrol potential of a broad spectrum atifungal strain Bacillus amyloliquefaciens B4 for postharvest loquat fruit storage,” Postharvest Biology and Technology, vol. 174, 111439, 2021.
. D. Aiello, C. Restuccia, E. Stefani, A. Vitale, and G. Cirvilleri, “Postharvest biocontrol ability of Pseudomonas synxantha against Monillinia fructicola and Monillinia fructigena on stone fruit,” Postharvest Biology and Technology, vol. 149, pp. 83-89, 2021.
. D. Romero, A. Pérez-García, M. E. Rivera, F. M. Cazorla, and A. De Vicente, “Isolation and evaluation of antagonistic bacteria towards the cucurbit powdery mildew fungus Podosphaera fusca,” Applied Microbiology and Biotechnology, vol. 64, no. 2, pp. 263-269, 2004.
. J. F. Shi and C. Q. Sun, “Isolation, identification, and biocontrol of antagonistic bacterium against Botrytis cinerea after tomato harvest.” Brazilian Journal of Microbiology, vol. 48, no.4, pp. 706-714, 2017.
. E. Chalutz and C. L. Wilson, “Postharvest biocontrol of green and blue mold and sour rot of citrus fruit by Debaryomyces hansenii,” Plant disease, vol. 74, no.2, pp. 134-137, 1990.
. W. You, C. Ge, Z. Jiang, M. Chen, W. Li, and Y. Shao, “Screening of a broad-spectrum antagonist Bacillus siamensis, and its possible mechanisms to control postharvest disease in tropical fruits,” Biological Control, vol. 157, 104584, 2021.