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Analysis of ethylene glycol in animal feed using liquid chromatography electrospray ionization tandem mass spectrometry

Nguyen Thi Hong Ngoc Nguyen Ha Thanh Bui Cao Tien Tran Cao Son Dinh Minh Nhat Pham Thi Thanh Ha
Published 12/24/2021

Article Details

How to Cite
Nguyen Thi Hong Ngoc, Nguyen Ha Thanh, Bui Cao Tien, Tran Cao Son, Dinh Minh Nhat, Pham Thi Thanh Ha. "Analysis of ethylene glycol in animal feed using liquid chromatography electrospray ionization tandem mass spectrometry". Vietnam Journal of Food Control. vol. 4, no. 4 (en), pp. 292-297, 2021
PP
292-297
Counter
532

Main Article Content

Abstract

A determination method of ethylene glycol in animal feed by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was developed. The analysis was performed with a C18 column (150 mm × 4.6 mm, 3.5 µm), and a gradient mobile phase of 0.1 % formic acid solution and acetonitrile. Ethylene glycol and surrogate standard (propylene glycol) were extracted by water at room temperature, derivatized with benzoyl chloride in alkaline medium, and analyzed by LC-ESI-MS/MS. The method has high specificity and selectivity with a detection limit of 0.1 mg/kg, linearity range of 1 - 20 µg/mL, repeatability of 3.08 - 4.41 %, and recovery of  91.1 - 102.0 %, which meet AOAC requirements. This method has been applied to analyze some marketed animal feed samples.

Keywords:

ethylene glycol, animal feed, LC-ESI-MS/MS

References

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