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Method validation for simultaneous quantification of some Human Milk Oligosaccharides (HMOs) in dietary supplements by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Nguyen Thi Hong Ngoc Mac Thi Thanh Hoa Tran Hung Son Ngo Manh Dung Cao Cong Khanh Vu Thi Thanh An Pham Thi Thanh Ha Le Thi Hong Hao
Published 03/19/2021

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How to Cite
Nguyen Thi Hong Ngoc, Mac Thi Thanh Hoa, Tran Hung Son, Ngo Manh Dung, Cao Cong Khanh, Vu Thi Thanh An, Pham Thi Thanh Ha, Le Thi Hong Hao. "Method validation for simultaneous quantification of some Human Milk Oligosaccharides (HMOs) in dietary supplements by liquid chromatography tandem mass spectrometry (LC-MS/MS)". Vietnam Journal of Food Control. vol. 4, no. 1, pp. 73-84, 2021

Main Article Content


The method of simultaneous quantification some of Human Milk Oligosaccharide in dietary supplements by LC-MS/MS is an accurate and effective method to quickly determine the content of 2'-Fucosyllactose (2 '-FL), Lacto-N-neotetraose (LNnT), Lacto-N-tetraose (LNT), 3'-Siallylactose (3'-SL) and 6'-Siallylactose (6'-SL) in both dietary supplements powder and liquid. The method has been developed and validated follow the AOAC International guidelines. The mobile phase system consists of 2 channels: channel A (0.1% formic acid) and channel B (acetonitril) connected to the HILIC column (3.5 μm, 2.1mm × 150 mm) and the MS detector. The time analysis is 10 minutes, this method can identify all 5 substances belonging to the group HMOs. The detection limit and quantitative limit for all 5’-FL, LNnT, LNT, 3’-SL, 6’-SL were 4 mg/kg and 10 mg/kg, respectively. The linear range of the method ranges from 0.4 µg/mL to 40 µg/mL. Other validation parameters include the accuracy (R% 98.8 -103%); The precision (RSD% 1.69 - 5.54%) can meet the requirement of AOAC. The method was applied in practice to analyze 25 supplementary food samples on the market gives the results of analyzing total HMOs in powdered samples about 0.1 – 0.3 g/100g and for liquid samples about 0.01 – 0.03 g/100mL.


2’-Fucosyllactose (2’-FL), lacto-N-tetraose (LNT), HMOs; dietary supplement, LC-MS/MS


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