The aim of this study was to identify and evaluate for antimicrobial activity and phytochemical properties of the extracts from some plants belonging to different species such as Piper betle L., Allium cepa L., Allium ascalonium L., Allium Sativum L., Curcuma longa L.; Paederia tomentosa L.; Wedelia chinensis M. and Chromolaena odorata L. These extracts have been prepared and the antimicrobial activities have been examined on some bacteria strains like Escherichia coli T1, Bacillus cereus M1; Salmonella sp. ST; Shigella sp. AT and one fungus strain Aspegillus flavus KN by agar disc diffusion method. Results showed that the extracts from total of 8 plant species have been inhibited the growth of all over the micro-organisms strains used in the test at a different ratio, but the ethanol extracts from Piper betle and Wedelia chinensis M. showed the highest effect. Also, the extracts then were tested for the presence of bioactive compounds. The results showed that there were tannins, flavones, carbohydrates, proteins, and oils in Wedelia chinensis M.extracts. In Piper betle extracts there were many phytochemical compounds including phenols, tannins, saponins, carbohydrates, proteins, resins, and oils.
Antimicrobial; extract; herbal medicine Piper betle L.; Wedelia chinensis M.
[1]. M. J. Lerma-Garcia, E. F. Simo-Alfonso, A. Mendez, J. L. Liberia and Jose Manuel Herrero-Marinez, “Fast separation and determination of sterols in vegetable oil by ultraperformance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry detection”, Journal of Agriculture and Food Chemistry, vol. 58, p. 2771-2776, 2010.
[2]. I. Borkovcava, E. Janouskova, M. Drackova, B. Janstove and L. Vorlova, "Determination of sterols in dairy products and vegetable fats by HPLC and GC methods", Czech Journal of Food Science, vol. 27, 2008.
[3]. AOAC Official Methods of Analysis, Appendix F: Guidelines for standard method performance requirements. 2012.
[4]. AOAC Official methods 2007.03, “Campesterol, Stigmaterol, and beta-sitosterol in saw palmetto raw materials and dietary supplements”, 2007.
[5]. Y-Z. Chen, S-Y. Kao, H-C. Jian, Y. Yu, J-Y. Li, W-H Wang and C-W. Tsai, “Determination of cholesterol and four phytosterols in food without derivatization by gas chromatography-tandem mass spectrometry”, Journal of Food and Drug Analysis, vol 23, no.4, pp.1-9, 2015.
[6]. Ruinan Yang, Li Xue, Liangxiao Zhang, Xuefang Wang, Xin Qi, Jun Jiang, Li Yu, Xiupin Wang, Wen Zhang, Qi Zhang and Peiwu Li, “ Phytosterol contents of edible oils and their contributions to extimated phytosterol intake in the Chinese diet”, Foods, vol. 8, no. 334, pp. 2-12, 2019.
[7]. S.L. Abidi, “Chromatographic analysis of plant sterols in foods and vegetable oils”, Journal of Chromatography A, vo. 935, pp. 173-201, 2001.
[8. B. Xu, S. You, L. Zhou, H. Kang, D. Luo, H. Ma and S. Han, “Simultaneous determination of free phytosterols and tocopherols in vegetable oils by an improved SPE-GC-FID method”, Food Ananlytical Methods, vol. 13, no.2, pp. 1-12, 2020.
[9]. N. Qi, Y. Liu, L. Liao, X. Gong and C. Yang, “A new method for determination of campesterol, stigmaterol and β-sitosterol in edible oils by suprcritical fluid chromatography”, Journal of Food and Nutrition Reseach, vol.58, no. 4, pp. 363-369, 2019.