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Authors |
Abstract
In this study, 36 yeast strains were isolated from fermented food sources collected in different areas of Northern Vietnam. The screening process yielded 15/36 strains capable of good growth with Cell Dry Weight (CDW) ranging from 10-14 g/L, and 10/36 strains having the potential for single cell protein production with Total Protein Content (TPC) exceeding 57% (g/100 g cell dry weight) after 24 hours of cultivation in YPD liquid medium. The yeast strain YPI-Y2 (with CDW reaching 14.03 g/L; TPC reaching 62.3%) was identified as closely related to the species Pichia kudriavzevii (99.77%) based on ITS rDNA sequence analysis and comparison. The nutritional and cultivation characteristics of the Pichia kudriavzevii YPI-Y2 strain, oriented towards cell biomass production and protein accumulation, were determined. The optimal cultivation temperature and initial medium pH condition for the growth and protein accumulation of the YPI-Y2 yeast strain were 30°C and 6.5, respectively, while the suitable carbon source was identified as D-fructose. Simultaneously, this yeast strain still demonstrated good growth and protein accumulation capability on inorganic nitrogen sources (except urea with 35.62% of TPC). Under suitable cultivation and nutritional conditions, the CDW and TPC values reached 16.12 g/L and 66.62 % (g/100g CDW), respectively.
Keywords: Single cell protein (SCP), Pichia kudriavzevii, yeast, fermented food, YPI-Y2.
References
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[2]. Jach, M.E., Serefko, A., Ziaja, M., and Kieliszek, M., “Yeast protein as an easily accessible food source”, Metabolites, vol. 12, no. 1, article 63, 2022.
[3]. Samiksha, F., San Valentin, E. M. D., Li, G., Blazer, M., McCormick, T. S., and Ghannoum, M., “A narrative review on the functional applications, safety, and probiotic characteristics of Pichia”, Nutrients, vol. 17, article 3594, 2025.
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[5]. Hải, P. T. M., Hải, N. T. T., and Uyên, L. N., “Sản xuất sinh khối nấm men Saccharomyces cerevisiae từ dịch thủy phân rong Ulva lactuca và thử nghiệm dùng trong nuôi lưu hàu Thái Bình Dương thương phẩm”, Tạp chí Khoa học Đại học Cần Thơ, vol. 60, số đặc biệt SDMD, pp. 138–145, 2024.
[6]. Bộ Công Thương, “Tận dụng phụ phẩm ngành giấy để sản xuất thức ăn chăn nuôi”. [Trực tuyến]. Địa chỉ: https://nq57.igip.gov.vn/tin-tuc/t3207 [Truy cập ngày 26 tháng 12 năm 2025].
[7]. Yunfei Chu, Mengmeng Li, Jiahui Jin, Xiameng Dong, Ke Xu, Libo Jin, Yanming Qiao, Hao Ji, “Advances in the Application of the Non-Conventional Yeast Pichia kudriavzevii in Food and Biotechnology Industries”, Journal of Fungi, vol.9, 170, 2023.
[8]. Epperson, L.E., and Strong, M., “A scalable, efficient, and safe method to prepare high quality DNA from mycobacteria and other challenging cells”, Journal of Clinical Tuberculosis and Other Mycobacterial Diseases, vol. 19, article 100150, 2020.
[9]. White, T.J., Bruns, T.D., Lee, S.B., and Taylor, J.W., “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics”, in PCR Protocols: A Guide to Methods and Applications, Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J. (eds.), Academic Press, New York, pp. 315–322, 1990.
[10]. Newman, L., Duffus, A.L.J., and Lee, C., “Using the free program MEGA to build phylogenetic trees from molecular data”, American Biology Teacher, vol. 78, no. 7, pp. 608–612, 2016.
[11]. Van-Thuoc, D., and Quillaguamán, J., “Improving culture conditions for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Bacillus sp. ND153, a bacterium isolated from a mangrove forest in Vietnam”, Annals of Microbiology, vol. 64, pp. 991–997, 2014.
[12] Viện Tiêu chuẩn chất lượng Việt Nam, “TCVN 10034:2013 (ISO 1871:2009) Thực phẩm và thức ăn chăn nuôi – Hướng dẫn chung về xác định hàm lượng nitơ bằng phương pháp Kjeldahl”, 2013.
[13] AOAC International, “AOAC Official Method 2001.11: Protein (Crude) in Animal Feed, Forage, Grain, and Oilseeds – Block Digestion Method Using Copper Catalyst and Steam Distillation into Boric Acid”, Official Methods of Analysis of AOAC INTERNATIONAL, 2023.
[14]. Rachamontree, P., Phusantisampan, T., Woravutthikul, N., Pornwongthong, P., and Sriariyanun, M., “Selection of Pichia kudriavzevii strain for the production of single-cell protein from cassava processing waste”, International Journal of Biological, Food, Veterinary and Agricultural Engineering, vol. 9, no. 5, 2015.
[15]. Nayana, K., Vidya, D., Soorya, K., et al., “Effect of volume and surface area on growth and productivity of microalgae in culture system”, BioEnergy Research, vol. 16, pp. 1013–1025, 2023.
[16]. Xu, Q., Huang, W., Li, Y. et al., “Isolation and identification of epiphytic Pichia kudriavzevii from loquat peels and investigation of its fermentation characteristics for liquor production”, Archives of Microbiology, vol. 206, article 440, 2024.
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[18]. Nieto-Sarabia, V. L., Ballinas-Cesatti, C. B., Melgar-Lalanne, G., Cristiani-Urbina, E., and Morales-Barrera, L., “Isolation, identification, and kinetic and thermodynamic characterization of a Pichia kudriavzevii yeast strain capable of fermentation”, Food and Bioproducts Processing, vol. 131, pp. 109–124, 2022.
[19]. Chamnipa, N., Thanonkeo, S., Klanrit, P., and Thanonkeo, P., “The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production”, Brazilian Journal of Microbiology, vol. 49, no. 2, pp. 378–391, 2018.
[20] Xu, T., Song, S., Ren, B., Li, J., Yang, J., Bai, L., and Piao, Z., “Fungus Pichia kudriavzevii XTY1 and heterotrophic nitrifying bacterium Enterobacter asburiae GS2 cannot efficiently transform organic nitrogen via hydroxylamine and nitrite”, Frontiers in Microbiology, vol. 13, article 1038599, 2022.
[21]. Jeppsson, M., Johansson, B., Hahn-Hägerdal, B., and Gorwa-Grauslund, M. F., “Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose”, Applied and Environmental Microbiology, vol. 68, no. 4, pp. 1604–1609, 2002.
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2017.
[24]. Koutinas, M., Patsalou, M., Stavrinou, S., and Vyrides, I.,“High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10”, Letters in Applied Microbiology, vol. 62, no. 1, pp. 75–83, 2016.
