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Simultaneous determination of Rutin, Hesperidin and Quercetin contents in solid dietary supplements by high performance liquid chromatography

Nguyen Thi Thanh Phuong Nguyen Thi Hong Hanh Dam Thi Thu
Received: 21 Jan 2020
Revised: 28 Feb 2020
Accepted: 14 Mar 2020
Published: 31 Mar 2020

Article Details

How to Cite
Nguyen Thi Thanh Phuong, Nguyen Thi Hong Hanh, Dam Thi Thu. "Simultaneous determination of Rutin, Hesperidin and Quercetin contents in solid dietary supplements by high performance liquid chromatography". Vietnam Journal of Food Control. vol. 3, no. 1, pp. 38-45, 2020

Main Article Content


Simultaneous determination of rutin, hesperidin and quercetin contents in several solid dietary supplement samples is possible by HPLC method. The analysis process usedthe Agilent Zorbax Eclipse XDB C18 column (250 x 4.6 mm; 5 µm), a mobile phase composed of acetonitrile - phosphoric acid solution 0.1% with gradient program. The detection was carried out on a DAD detector at 280 nm for hesperidin and at 254 nm for rutin and quercetin. The limit of quantitation of method was at a low level of concentration (0.021g/100g with rutin, 0.047g/100g with hesperidin and 0.0054g/100g with quercetin); the accuracy of the method was within 97.0 - 101.33%; the precision showed 1.11 - 1.92% of relative standard deviation which showed high repeatability of the method. The method was applied to 20 dietary supplements samples, the results indicated a high level for the selectivity and reliability of the method to be applied.


HPLC, rutin, hesperidin, quercetin, supplements


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