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Simultaneous determination of steviol glycosides in stevia by high performance liquid chromatography

Luu Thi Huyen Trang Doan Thi Thanh Huong Vu Thi Trang Tran Cao Son Nguyen Thi Anh Huong Nguyen Thi Minh Loi
Published 11/26/2019

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Luu Thi Huyen Trang, Doan Thi Thanh Huong, Vu Thi Trang, Tran Cao Son, Nguyen Thi Anh Huong, Nguyen Thi Minh Loi. "Simultaneous determination of steviol glycosides in stevia by high performance liquid chromatography". Vietnam Journal of Food Control. vol. 2, no. 4, pp. 2-8, 2019

Main Article Content


A method for determination of steviol glucosides in stevia using high performance liquid chromatography was developed and validated. The compounds were extracted from the matrices with methanol for 60 minutes, cleaned by Oasis HLB column and then determined by HPLC using C18 column (250 mm x 4.6 mm x 5 µm) and PDA detector. The calibration curves were linear in range of 1 to 100 μg/mL; the RSD was of 1.39 ­ 2.85%; and the recovery was of 93 ­ 105%. The method was applied to analyze 09 stevia samples collected from markets in Hanoi (including dry stevia rebaudiana, stevia powder). The results showed that composition of steviol glycosides differs from sample to sample. Besides, stevioside and rebaudioside A are the most abundant steviol glycosides in the samples.


HPLC, steviol glycosides, stevia


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