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Determination of sialic acid in dietary supplement by liquid chromatography tandem-mass spectrometry (LC-MS/MS)

Nguyen Thi Hong Ngoc Mac Thi Thanh Hoa Duong Thi Mai Hoa Tran Hung Son Cao Cong Khanh Pham Thi Thanh Ha Le Thi Hong Hao
Received: 02 Jul 2019
Revised: 10 Sep 2019
Accepted: 18 Sep 2019
Published: 01 Oct 2019

Article Details

How to Cite
Nguyen Thi Hong Ngoc, Mac Thi Thanh Hoa, Duong Thi Mai Hoa, Tran Hung Son, Cao Cong Khanh, Pham Thi Thanh Ha, Le Thi Hong Hao. "Determination of sialic acid in dietary supplement by liquid chromatography tandem-mass spectrometry (LC-MS/MS)". Vietnam Journal of Food Control. vol. 2, no. 3, pp. 3-9, 2019

Main Article Content


Simultaneous determination of sialic acids (N-acetyl neuraminic acid - NANA and N-glycolyl neuraminic acid - NGNA) in dietary supplement matrices by liquid chromatography tandem-mass spectrometry (LC-MS/MS) had been developed. Sialic acids were separated by using a C18 column (75 mm x 3.9 mm, 2.5 µm) and isocratic mobile phase of acid formic 0.1 %: acetonitrile (80:20, v/v). Sialic acids were totally hydrolyzed by formic acid 1M at 80oC in 2 hours before being analyzed by LC-MS/MS. It was proved that the method with the linearity range of 0.04 - 4.25 µg/ml had highly specificity, selectivity, repeatability, and recovery rate, that well satisfied AOAC requirements. The method has been applied to detect sialic acids in commercial dietary supplements.


Sialic acid, N-acetyl neuraminic acid, NANA, N-glycolyl neuraminic acid, NGNA, dietary supplement


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