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Quantitative determination of curcumin, demethoxycurcumin and bisdemethoxycurcumin from dietary supplements using HPLC-UV

Nguyen Huong Giang Do Ngoc Nhan Pham Van Son Nguyen Bui Duy Ngo Thi Lu Le Minh Hai
Received: 01 Jul 2019
Revised: 04 Sep 2019
Accepted: 12 Sep 2019
Published: 01 Oct 2019

Article Details

How to Cite
Nguyen Huong Giang, Do Ngoc Nhan, Pham Van Son, Nguyen Bui Duy, Ngo Thi Lu, Le Minh Hai. "Quantitative determination of curcumin, demethoxycurcumin and bisdemethoxycurcumin from dietary supplements using HPLC-UV". Vietnam Journal of Food Control. vol. 2, no. 3, pp. 10-17, 2019
PP
10-17
Counter
736

Main Article Content

Abstract

A simple, sensitive and reliable method was developed and applied to determine curcumin, demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC) (curcuminoid) in dietary supplements by High-Performance Liquid Chromatography using UV detector (HPLC-UV). The method was validated for linearity, repeatability, reproducibility, limits of detection and limits of quantification. The limits of detection and limits of quantification of curcuminoids were found to be 10 mg/kg and 40 mg/kg, respectively. The calibration curve showed good linearity for the three compounds (R2 > 0,999). The average recovery rates of curcuminoid at three spiked levels ranged from 90.3% to 106.7% and the relative standard deviations were less than 2.0%. The method has also been successfully applied to analyze curcuminoid in the real samples collected in Ho Chi Minh City.

Keywords:

Curcumin, demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), dietary supplements, HPLC-UV

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