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Simultaneous determination of fatty acid esters 2-chloro-1, 3-propandiol(2-mcpd), 3-chloro-1,2- propandiol(3-mcpd) and glycidol in vegetable oils by gas chromatography mass spectrometry GC-MS/MS

Le Thanh Tam Nguyen Thanh Duy Ly Tuan Kiet Phu Minh Tan Nguyen Quoc Hung Chu Van Hai
Received: 02 Jul 2019
Revised: 17 Sep 2019
Accepted: 27 Sep 2019
Published: 01 Oct 2019

Article Details

How to Cite
Le Thanh Tam, Nguyen Thanh Duy, Ly Tuan Kiet, Phu Minh Tan, Nguyen Quoc Hung, Chu Van Hai. "Simultaneous determination of fatty acid esters 2-chloro-1, 3-propandiol(2-mcpd), 3-chloro-1,2- propandiol(3-mcpd) and glycidol in vegetable oils by gas chromatography mass spectrometry GC-MS/MS". Vietnam Journal of Food Control. vol. 2, no. 3, pp. 67-73, 2019
PP
67-73
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497

Main Article Content

Abstract

The food contaminants including 3-chloro-1,2-propanediol (3-MCPD) and 3-MCPD fatty acid esters have been a matter of great concern in the past few years due to their toxicity and their occurrence in numerous foods. Recently, significant amounts of the isomeric compounds 2-chloro-1,3-propanediol (2-MCPD) fatty acid esters have been detected in refined oil samples.

Gas Chromatography (GC) coupled with mass spectroscopy (MS) was used for the quantification of fatty acid esters of 2-Chloro-1,3-propandiol (2-MCPD), 3-Chloro-1,2- propandiol (3-MCPD) and glycidol in oil. Internal standards including 3-MCPD-ester-d5 (rac 1,2-bis-palmitoyl-3-chloropropanediol-d5), 2-MCPD-ester-d5 (1,3-Distearoyl-2- chloropropanediol-d5) and Glycidyl sterate-d5 were added to samples. Spiked samples were, then, incubated in NaBr/H2SO4 and H2SO4/MeOH solutions. They were, eventually, extracted with ethyl acetate and n-hexane solvents in order to convert Glycidyl esters to free MCPD and glycidol in samples before using phenylboronic acid derivative. The limit of quantitation was of 0.1 ppm (mg/kg). The recoveries at the concentration of 0.1 mg/kg ranged from 80 to 120% and the repeatability and reproducibility were less than 10%.

Keywords:

2-Chloro-1,3-propandiol (2-MCPD), 3-Chloro-1,2-propandiol (3-MCPD), glycidol, phenylboronic acid, gas chromatography mass spectrometry

References

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