Bìa tạp chí



Simultaneous determination of steviol glycosides in food from stevia by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Luu Thi Huyen Trang Vu Thi Kim Oanh Vu Thi Trang Nguyen Thi Bien
Received: 27 Mar 2019
Revised: 23 May 2019
Accepted: 31 May 2019
Published: 31 May 2019

Article Details

How to Cite
Luu Thi Huyen Trang, Vu Thi Kim Oanh, Vu Thi Trang, Nguyen Thi Bien. "Simultaneous determination of steviol glycosides in food from stevia by liquid chromatography tandem mass spectrometry (LC-MS/MS)". Vietnam Journal of Food Control. vol. 2, no. 2 (en), pp. 23-28, 2019

Main Article Content


The objective of this study is to develop a method for determination of steviol glycosides in food using liquid chromatography mass tandem spectrometry. The compounds were extracted from the matrices with methanol at 40°C within 60 minutes, and then determined by LC-MS/MS using C18 column (100 mm x 2.1 mm x 3.5 µm), the MS/MS detector with negative ESI mode. The calibration curves were linear in the range of 0.1 to 20 μg/mL; the RSD was of 1.74 – 5.01%; and the recovery was in range of 90 – 105%. The method was applied to analyze 20 food samples collected from markets in Hanoi (including dry stevia rebaudiana, stevia powder, stevia tea and soft drinks). The results showed that the composition of steviol glycosides was different from sample to sample. The most abundant steviol glycosides were stevioside and rebaudioside A.


LC-MS, steviol glycosides, stevia


1. Tôn Nữ Liên Hương, Võ Hoàng Duy, Dương Mộng Hòa, Đỗ Duy Phúc và Nguyễn Duy Thanh, (2015) “Chiết xuất Stevioside từ cây cỏ ngọt”, Tạp chí khoa học trường đại học Cần Thơ, Phần A: Khoa học tự nhiên, công nghệ và môi trường: 36 (2015):73-76
2. Nguyễn Kim Cẩn, Lê Nguyệt Nga (2001),“Định lượng stevioside trong lá cỏ ngọt”, Công trình nghiên cứu khoa học 1987-2000, Viện dược liệu, Nhà Xuất bản Khoa học và Kỹ thuật: 125-128.
3. Annie Shirwaikar, Vinit Parmar, Jay Bhagat and Saleemulla Khan ( 2011), “Identification and estimation of stevioside in the commercial samples of stevia leaf and powder by HPTLC and HPLC,Int. J. of Pharm. & Life Sci. (IJPLS), 2, 9, 1050-1058.
4. Claudio Gardana, Martina Scaglianti, Paolo Simonetti (2010) “Evaluation of steviol and its gly cosides in Stevia rebaudiana leaves and commercial sweetener by ultra-high-performance liquid chromatography-mass spectrometry” Journal of Chromatography A, 1217 (2010) 1463–1470.
5. Molina-Calle, M., Sánchez de Medina, V., Delgado de la Torre, M. P., Priego-Capote, F., & Luque de Castro, M. D. (2016). “Development and application of a quantitative method based on L–QqQ MS/MS for determination of steviol glycosides in Stevia leaves”. Talanta, 154(C), 263–269.
6. Paweł Kubica, Jacek Namieśnik, Andrzej Wasik (2015) “Determination of eight artificial sweet eners and common Stevia rebaudiana glycosides in non-alcoholic and alcoholic beverages by reversed-phase liquid chromatography coupled with tandem mass spectrometry” Anal Bioanal Chem, 2015 Feb, 407(5):1505-12
7. R. Amery, E. Jooken, J. Geuns4 and B. Meesschaert. “Determination of steviol glycosides in Various dairy matrices and soy drink”. Centre for Surface Chemistry and Catalysis and Leuven Food Science and Nutrition Research Centre; 2010; Vol. 4; pp.69-82