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Determination of xanthophylls compounds in dietary supplements by HPLC-PDA

Mac Thi Thanh Hoa Nguyen Thi Hong Ngoc Duong Thi Mai Hoa Cao Cong Khanh
Published 05/10/2019

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How to Cite
Mac Thi Thanh Hoa, Nguyen Thi Hong Ngoc, Duong Thi Mai Hoa, Cao Cong Khanh. "Determination of xanthophylls compounds in dietary supplements by HPLC-PDA". Vietnam Journal of Food Control. vol. 2, no. 2 (en), pp. 51-56, 2019

Main Article Content


An HPLC method using photodiode array detector (PDA) detector has been developed for the determination of astaxanthin, lutein, and zeaxanthin in dietary supplements. These compounds were separated on a C30 chromatography column with ethyl acetate: acetonitrile (12:88, v/v) containing 0.1% n-decanol as the mobile phase. Xanthophyll compounds, usually existing in the form of esters, were saponified in 45% KOH solution (with lutein and zeaxanthin) and 1% (with astaxanthin) at 60°C within 15 minutes, then re-extracted with n-hexane before analyzing on HPLC system. The method was validated and had good specificity and selectivity. The linear calibration curve in the range of 0.5 - 10 µg/ml, the repeatability and the recovery of the method meet the analytical requirements according to AOAC; the method was applied to analyze several dietary supplements collected from market.


xanthophyll, lutein, zeaxanthin, astaxanthin, dietary supplement, HPLC


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