Bìa tạp chí

 

009bet

Simultaneous determination of pyrrolizidine alkaloids in food supplements of herbal origin using liquid chromatography tandem mass spectrometry

Tran Cao Son Bui Cao Tien Dang Phuong Thao Nguyen Xuan Truong Cung Thi To Quynh Le Thi Hong Hao
Received: 04 Jan 2019
Revised: 11 Feb 2019
Accepted: 18 Feb 2019
Published: 28 Feb 2019

Article Details

How to Cite
Tran Cao Son, Bui Cao Tien, Dang Phuong Thao, Nguyen Xuan Truong, Cung Thi To Quynh, Le Thi Hong Hao. "Simultaneous determination of pyrrolizidine alkaloids in food supplements of herbal origin using liquid chromatography tandem mass spectrometry". Vietnam Journal of Food Control. vol. 2, no. 1, pp. 24-31, 2019
PP
24-31
Counter
682

Main Article Content

Abstract

A simple and accurate method has been developed for the simultaneous determination of four pyrrolizidine alkaloids (PAs) including echimidine, senecionine, jacobine and intermidine in food supplements of plant origin. The instrumental conditions including the use of TSK-Gel column for the separation and an MS/MS detector in positive ESI source, multi-reactive monitoring (MRM) mode for quantitation were optimized. The method has fully validated and meets the performance requirement of AOAC with good specificity. The linearity varies from 1.0 µg/kg to 40 µg/kg, the LOD was of 0.3 µg/kg; the repeatability and recovery range from 3.6% to 7.5% and 88.7% to 117%, respectively. The method was applied to determine PAs in 90 food supplement samples, of which 26 samples (28.8%) contain PAs.

Keywords:

LC-MS/MS, pyrrolizidine alkaloids, senecionine, jacobine, intermidine, echimidine

References

1. Edgar J. A., Colegate S. M., Boppré M., Molyneux R. J. (2011), “Pyrrolizidine alkaloids in food: a spectrum of potential health consequences”, Food Additives & Contaminants: Part A, vol. 28, no. 3, pp. 308–324.
2. Poppenga R. H., Puschner B. (2009), “Poisonous Plant Threats to Cattle and Horses: Tansy Ragwort, Common Groundsel and Fiddleneck,” California Animal Health and Food Safety Laboratory System (CAHFS).
3. EFSA (2007), “Opinion of the scientific panel on contaminants in the food chain on a request from the European Commission related to pyrrolizidine alkaloids as undesirable substances in animal feed”, EFSA Journal, vol. 447, pp. 1–51.
4. TCVN 12053:2017 (CAC/RCP 74-2014), “Quy phạm thực hành kiểm soát cỏ dại để ngăn ngừa và giảm thiểu nhiễm alkaloid pyrrolizidine trong thực phẩm và thức ăn chăn nuôi”, Bộ Khoa học và Công nghệ.
5. Kempf M. et al. (2008), “Pyrrolizidine alkaloids in honey: Risk analysis by gas chromatography-mass spectrometry” Molecular nutrition & food research, vol. 52, no. 10, pp. 1193–1200.
6. Crews C., Startin J. R., Clarke P. A. (1997), “Determination of pyrrolizidine alkaloids in honey from selected sites by solid phase extraction and HPLC-MS”, Food Additives & Contaminants, vol. 14, no. 5, pp. 419–428.
7. Edgar J. A., Colegate S. M., Boppré M., Molyneux R. J. (2011), “Pyrrolizidine alkaloids in food: a spectrum of potential health consequences”, Food Additives & Contaminants: Part A, vol. 28, no. 3, pp. 308–324.
8. Griffin C. T., Gosetto F., Danaher M., Sabatini S., Furey A. (2014), “Investigation of targeted pyrrolizidine alkaloids in traditional Chinese medicines and selected herbal teas sourced in Ireland using LC-ESI-MS/MS”, Food Additives & Contaminants: Part A, vol. 31, no. 5, pp. 940–961.
9. Zhou Y. et al. (2010), “A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography–tandem quadrupole mass spectrometry” Analytica chimica acta, vol. 681, no. 1–2, pp. 33–40.

 Submit