Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
Bioactive peptides are short amino acid sequences involved in regulating key physiological processes, including cellular signaling and metabolic functions. Despite their small size, these peptides demonstrate high specificity and significant biological activity through receptor interactions. Silkworm pupae are a rich source of proteins, lipids, carbohydrates, minerals, vitamins, and polyphenolic compounds, making them a promising raw material for peptide extraction. However, effective extraction requires an initial pretreatment step to enhance raw material preservation and facilitate lipid removal, which otherwise impairs protease activity during enzymatic hydrolysis. In this study, lipase-assisted hydrolysis was employed to remove lipids from silkworm pupae powder. Using lipase (CAS 9001-62-1), optimal conditions were established at 50℃, 1.5% enzyme concentration, and 2 hours of reaction time. This pretreatment significantly improved protease hydrolysis efficiency, resulting in a 44% increase in amino nitrogen yield compared to untreated samples.
Peptide, enzyme, amino acid, lipase, silkworm pupae.
[1]. K.P. Singh and R.S. Jayasomu, “Bombyx mori - A Review of its Potential as a Medicinal Insect,” Pharmaceutical Biology, vol. 40, no. 1, pp. 28–32, 2002.
[2]. Xiaolin Wu, Jing Yang, William MumbY, Yuhong Zhang, Yu Zhang, Chen Wang, Xiaoyong Chen, Huayi Suo, Jiajia Song, “Silkworm pupa protein and its peptides: Preparation, biological activity, applications in foods, and advantages,” Trends in Food Science & Technology, Vol. 139, pp. 104129, 2023.
[3]. Suling Sun, Wei Wang, Nan Wang, Yu Zhang, Zuoyi Zhu, Xue Li, Junhong Wang, Qihe Chen, Faizan Ahmed Sadiq, Hua Yang, Qianhui Qi, Guohua Zhang, “HPP and SGQR peptides from silkworm pupae protein hydrolysates regulated biosynthesis of cholesterol in HepG2 cell line,” Journal of Functional Foods, vol. 77, 2021.
[4]. Li Z., Zhao S., Xin X., Zhang B., Thomas A., Charles A., Lee K.S., Jin B.R., Gui Z. Purification, “Identification and Functional Analysis of a Novel Immunomodulatory Peptide from Silkworm Pupa Protein,” International Journal of Peptide Research and Therapeutics, vol. 26, pp. 243–249, 2020.
[5]. Zhou Y., Ji X., Wang D., Guo Y., Zhao J., Yan W, “Effect of Silkworm Pupae (Bombyx mori) Protein on Colon Cancer in Nude Mice: Inhibition of Tumor Growth, Oxidative Stress and Inflammatory Response,” Frontiers in Pharmacology, vol. 14, 2023.
[6]. Maria Cermeño, Carmen Bascón, Miryam Amigo-Benavent, Manuel Felix, Richard J. FitzGerald, “Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity,” Journal of Functional Foods, vol. 92, 2022.
[7]. Qing-Lei Zeng, Ning Zhang, Yue-Yue Zhang, Xiang-Dong Xin, Thomas Attaribo, Ying Shao, Liu-Mei Tang, Ran Zhang, Kwang Sik Lee, Byung Rae Jin, Zhongzheng Gui, “Ionic liquid extraction of silkworm pupa protein and its biological characteristics,” Journal of Asia-Pacific Entomology, Vol. 24, iss. 1, pp. 363-368, 2021.
[8]. Isabella Pali-Schöll, Eva Untersmayr, Martina Klems and Erika Jensen-Jarolim, “The Effect of Digestion and Digestibility on Allergenicity of Food,” Nutrients, vol. 10, no. 9, 2018.
[9]. KM Ji, Z-K Zhan, J-J Chen, Z-G Liu, “Anaphylactic shock caused by silkworm pupa consumption in China,” Allergy, vol. 63, no. 10, 2008.
[10]. [10] Van der Poel, L., Chen, J. and Penagos, M, “Food Allergy Epidemic—Is It Only a Western Phenomenon?,” Current Allergy & Clinical Immunology, vol. 22, no. 3, 2009.
[11]. Kuniyoshi Shimakura, Yumiko Tonomura, Yuki Hamada, Yuji Nagashima, Kazuo Shiomi (2005). “Allergenicity of crustacean extractives and its reduction by protease digestion,” Food Chemistry, Vol. 91, iss. 2, pp. 247-253, 2005.
[12]. Yuan-Yuan Huang, Guang-Ming Liu, Qiu-Feng Cai, Wu-Yin Weng, Soheila J. Maleki, Wen-Jin Su, MinJie Cao, “Stability of major allergen tropomyosin and other food proteins of mud crab (Scylla serrata) by in vitro gastrointestinal digestion,” Food and Chemical Toxicology, vol. 48, iss. 5, pp. 1196-1201, 2010.
[13]. Thirupathaiah Yeruva, Harishkumar Jayaram, Ravindra Aurade, Manthira Moorthy Shunmugam, Vijay S Shinde, Sathyendra Rao Baragi Venkatesharao, Mary Josepha Azhiyakathu, “Profiling of nutrients and bioactive compounds in the pupae of silkworm, Bombyx mori,” Food Chemistry Advances vol. 3, 2023.
[14]. X. MeiS. CaiH. Wu, “Volatile components and in Vitro digestibility of silkworm pupae protein,” Modern Food Science and Technology, Beijing University, vol. 31, no. 10, 2015.
[15]. Yan Dai, Meijia Huang, Yujuan Xu, Lixia Mu, Jingyan Gao, Hongbing Chen, Zhihua Wu, Anshu Yang, Yong Wu, Xin Li, “Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes,” Food Science and Human Wellness, vol. 12, iss. 3, pp. 774-782, 2023.
[16]. Vu Kim Thoa, Nguyen Thi Phuong Thao, Nguyen Thi Thu Hien, "Research on the Acquisition of Amino Acid-Rich Extracts from Silkworm Pupae by Enzymes," Vietnam Journal Online, vol. 95, pp. 12 – 20, 2022 [in Vietnamese].
[17]. TCVN 6120:2018 (ISO 662:2016) - Animal and vegetable oils and fats - Determination of moisture and volatile content [in Vietnamese].
[18]. Paul D., Dey S., “The essential amino acids, lipid profile, and fat-soluble vitamins of edible silkworms Bombyx mori (Lepidoptera: Bombycidae),” International Journal of Tropical Insect Science, vol. 34, pp. 239–247, 2014.
[19]. Wang W., Xu L., Zou Y., Pang D., Shi W., Mu L., Li E., Lan D., Wang Y., Liao S, “Comprehensive identification of the main lipid layers and tocochromanols in silkworm oil (Antheraea pernyi and Bombyx mori),” European Journal of Lipid Science & Technology, vol. 122, 2020.
[20]. Lina Zhao, Baoxiang Wu, Shuyun Liang, Douyong Min, Hongrui Jiang,s “Insight of Silkworm Pupa Oil Regulating Oxidative Stress and Lipid Metabolism in Caenorhabditis elegans”. Foods, vol. 11, iss. 24, pp. 4084, 2022.