Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
α-Amylase is an endo-acting enzyme that hydrolyzes α-1,4-glucan linkages in starch and has broad applications in the beverage, food, and biomedical industries. Conventional quantification techniques (e.g., spectroscopy, ELISA) typically require expensive instrumentation, complex workflows, and long analysis times. Here, we present a flexible electrochemical sensor based on laser-scribed porous graphene on a polymer substrate, which increases the electroactive surface area and enhances interfacial electron transfer, thereby improving sensitivity. α-Amylase is quantified indirectly via the amount of maltose produced during enzyme-catalyzed starch hydrolysis in neutral buffer; maltose subsequently reduces K3[Fe(CN)6] to K4[Fe(CN)6] in alkaline medium, and the electrochemical oxidation current of K4[Fe(CN)6] is proportional to α-amylase activity. Under optimized conditions, the sensor achieves a limit of detection (LOD) of 0.61 U/mL and a limit of quantification (LOQ) of 1.08 U/mL, demonstrating its potential as a simple, low-cost, and point-of-care–deployable approach for α-amylase determination in food, industrial, and clinical samples.
α-amylase, electrochemical sensor on flexible substrate, porous graphene.
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