Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
Purple brown rice is a highly nutritious food source, rich in bioactive compounds, particularly anthocyanins - powerful antioxidants that play a crucial role in health protection. The production of sterilized purple rice milk in glass bottles not only provides a convenient, safe product with an extended shelf life but is also environmentally friendly. This study aimed to evaluate the effects of enzymatic hydrolysis temperature (75, 80, and 85℃) and time (5, 15, and 25 minutes) on the physicochemical properties of hydrolysates (soluble solids content, insoluble solids content, reducing sugar content, anthocyanin content, recovery yield, transmittance, and color values L, a*, b*, and Ho), as well as the sensory attributes of sterilized purple rice milk. The results showed that hydrolysis temperature had a significant impact on recovery yield, transmittance, and color values, whereas hydrolysis time clearly influenced soluble solids content, insoluble solids content, reducing sugars, and anthocyanin content. Under optimal conditions (80℃ for 15 minutes), the purple rice hydrolysate achieved a soluble solid content (7.27%), recovery yield (65.6%), reducing sugar content (29.2 mg/L), and anthocyanin content (11.8 mg/L). Furthermore, rice milk produced from this hydrolysate exhibited the highest sensory quality.
Anthocyanin, enzymatic hydrolysis, sensory attributes, recovery yield, purple rice milks.
[1]. E.-S. M. Abdel-Aal, J. C. Young, and I. Rabalski, “Anthocyanin composition in black, blue, pink, purple, and red cereal grains,” Journal of agricultural and food chemistry, vol.54, no.13, pp. 4696-4704, 2006
[2]. J. He and M. M. Giusti, “Anthocyanins: Natural colorants with health-promoting properties,” Annual Review of Food Science and Technology, vol.1, pp.163–187, 2010.
[3]. M. U. Ukwuru and A. C. Ogbodo, “Effect of processing treatment on the quality of tigernut milk,” Pakistan Journal of Nutrition, vol.10, no.1, pp.95-100, 2011.
[4]. H. Zhaohua, Q. Peiyou, Z. Yan, C. Songhuan and R. Guixing, “Identification of anthocyanins isolated from black rice (Oryza sativa L.) and their degradation kinetics,” Food Research International, vol.50, pp. 691–697, 2013.
[5]. C. Cheowtiraku, P. Chaijany, P. Jirathumkitku, K. Kriangkraiphipha, M. Ampunsang and D. Ruangcha, “A study on rice-milk production,” Australia Journal of technology, vol. 4, no. 3, pp. 143-156, 2001.
[6]. M. J. E. C. Van der Maarel, B. Van der Veen, J. C. M. Uitdehaag, H. Leemhuis and L. Dijkhuizen, “Properties and applications of starch-converting enzymes of the α-amylase family,” Journal of Biotechnology, vol. 94, no. 2, pp. 137–155, 2002.
[7]. A. Banyavongsa, T. T. T. Linh, N. Van Tai and N. M. Thuy, “Response surface optimization of enzymatic hydrolysis of purple rice (cultivated in Soc Trang, Vietnam) using amylases and formulation of healthy rice milk,” International Journal of Advances in Agricultural Science and Technology, vol. 6, no.10, pp. 17-28, 2019.
[8]. Nguyen Duc Luong. Enzyme Technology, Ho Chi Minh City University Publishing House, 2004 [in Vietnamese].
[9]. A. Patras, N. P. Brunton, C. O’Donnell and B. K. Tiwari, “Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradation,” Trends in Food Science & Technology, vol. 21, no. 1, pp. 3–11, 2010.
[10]. A. B. Arif, K. S. Sasmitaloka and C. Winarti, “Effect of liquefaction time and enzyme addition on liquid sugar production from sweet sorghum starch by enzymatic hydrolysis,” In IOP Conference Series: Earth and Environmental Science, vol. 250, no. 1, pp. 012042, 2019.
[11]. E. K. Hadde, T. M. Nicholson & J. A. Y. Cichero, “Rheological characterisation of thickened fluids under different temperature, pH and fat contents,” Nutrition & Food Science, vol. 45, no. 2, pp. 270-285, 2015
[12]. J. Gajdoš Kljusurić, M. Benković and I. Bauman “Classification and processing optimization of barley milk production using NIR spectroscopy, particle size, and total dissolved solids analysis,” Journal of Chemistry, vol.1, pp. 1-7, 2015.
[13]. O. Luigi. AOAC: Official methods of Analysis, AOAC 967.21 IFU Method No 17. The Association of official chemists 18th ed. Arlington, USA, 2004.
[14]. V. Paul, A. Singh & R. Pandey, “Estimation of total soluble solids (TSS) laboratory manual on post-harvest physiology of fruits and flowers,” Indian Agricultural Research Institute, pp. 41-43, 2010.
[15]. A. Negrulescu, V. Patrulea, M. M. Mincea, C. Ionascu, B. A. Vlad-Oros & V. Ostafe, “Adapting the reducing sugars method with dinitrosalicylic acid to microtiter plates and microwave heating,” Journal of the Brazilian Chemical Society, vol. 23, pp. 2176-2182, 2012.
[16]. J. Lee, R. W. Durst and R. E. Wrolstad, “Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study,” Journal of AOAC INTERNATIONAL, Vol. 88, Iss. 5, pp. 1269–1278, 2005.
[17]. J. Pinyo, P. Luangpituksa, M. Suphantharika, C. Hansawasdi and R. Wongsagonsup, “Effect of enzymatic pretreatment on the extraction yield and physicochemical properties of sago starch,” Starch‐Stärke, vol. 68, no, 1-2, pp. 47-56, 2016.
[18]. J. H. Hardesty and B. Attili, “Spectrophotometry and the Beer-Lambert Law: An important analytical technique in chemistry,” Collin College, Department of Chemistry, pp. 1-6, 2010.
[19]. A. Rodríguez-Mena, L. A.℃hoa-Martínez, S. M. González-Herrera, O. M. Rutiaga-Quiñones, R. F. González-Laredo, B. Olmedilla-Alonso and S. Vega-Maturino, “Coloring potential of anthocyanins from purple sweet potato paste: Ultrasound-assisted extraction, enzymatic activity, color and its application in ice pops,” Food Chemistry Advances, vol.3, 2023.
[20]. M. I. Abou-Dobara, M. M. Ismail & N. M. Refaat, “Chemical composition, sensory evaluation and starter activity in cow, soy, peanut and rice milk,” Journal of Nutritional Health & Food Engineering, vol. 5, no. 3, pp. 1 - 8, 2016.
[21]. S. L. Hii, J. S. Tan, T. C. Ling and A. B. Ariff, “Pullulanase: role in starch hydrolysis and potential industrial applications,” Enzyme Research, vol. 1, 2012.
[22]. S. R. Pillai, N. Venkatachalapathy, K. S. Kumar and A. Pare, “Effect of roasting and cooking on physiochemical properties of black rice soluble extract,” International Journal of Chemical Studies, vol. 9, no. 1, pp. 2848-2852, 2021.
[23]. G. Pereira-Caro, S. Watanabe, A. Crozier, T. Fujimura, T. Yokota and H. Ashihara, H, “Phytochemical profile of a Japanese black–purple rice,” Food Chemistry, vol. 141, no. 3, pp. 2821-2827, 2013.
[24]. A. L. D. S. Dias, B. Pachikian, Y. Larondelle and J. Quetin-Leclercq, “Recent advances on bioactivities of black rice, ” Current Opinion in Clinical Nutrition & Metabolic Care, vol. 20, no. 6, pp. 470-476, 2017.
[25]. S. I. F. S Martins, W. M. F. Jongen and M. A. J. S. van Boekel, “A review of Maillard reaction in food and implications to kinetic modelling,” Trends in Food Science & Technology, vol. 11, no. 9-10, pp. 364–373, 2000.
[26]. Duong Thi Ngoc Hanh and Nguyen Minh Thuy, "Use of enzyme – amylase in the hydrolysis of starch from dragon's blood rice starch," Journal of Science of Can Tho University, pp. 61-67, 2014 [in Vietnamese].
[27]. H. Wang, G. Cao and R. L. Prior, “Oxygen radical absorbing capacity of anthocyanins,” Journal of Agricultural and Food Chemistry, vol. 45, no.2, pp. 304–309, 2013.
[28]. S. Ngamsuk, J. L. Hsu, T. C. Huang and P. Suwannaporn, “Ultrasonication of milky stage rice milk with bioactive peptides from rice bran: Its bioactivities and absorption,” Food and Bioprocess Technology, vol. 13, pp. 462-474, 2020.
[29]. J. Y. Kim, Y. K. Lee and Y. H. Chang, “Structure and digestibility properties of resistant rice starch cross-linked with citric acid,” International Journal of Food Properties, vol. 20, no.2, pp. 2166-2177, 2017.
[30]. A. Castañeda-Ovando, M. D. L. Pacheco-Hernández, M. E. Páez-Hernández, J. A. Rodríguez and C. A. Galán-Vidal, “Chemical studies of anthocyanins: A review,” Food Chemistry, vol.113, no. 4, pp. 859–871, 2009.
[31]. G. Mazza and E. Miniati, “Anthocyanins in fruits, vegetables, and grains,” CRC Press, 1993.