Website: https://vjfc.nifc.gov.vn/
Website: https://vjfc.nifc.gov.vn/
This study aims to develop a method for simultaneous determination of some monoglyceride (MG) and diglyceride (DG) emulsifiers including: monoolein (MO), monostearin (MS), monopalmitin (MP), dipalmitin (DP) in milk by liquid chromatography-mass spectrometry. The sample was ultrasonically extracted with a solvent mixture of chloroform: methanol (MeOH) in the ratio of 2:1 at 45℃ for 30 minutes. The extract was evaporated, dissolved with MeOH and analyzed on a liquid chromatography-mass spectrometry system with the following conditions: C18 chromatography column (2.1 mm x 150 mm; 3.5 µm), mobile phase was 100% MeOH, flow rate was 0.3 mL/min, MS detector was in atmospheric pressure chemical ionization mode (APCI). The method had good specificity, the standard curve had a coefficient of R2 ≥ 0.9995. The repeatability was in the range of 2.54 - 7.21%, the recovery was in the range of 80.4 -99.5%, the limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.6 mg/kg and 0.2 mg/kg for MO, MP, MS, respectively; 0.15 mg/kg and 0.5 mg/kg for DP. The method was applied to analyze the emulsifiers in 20 different milk samples collected in Hanoi. The total content of 04 emulsifiers ranged from 4.51 - 42.2 mg/kg for powder milk and 4.67 - 12.6 mg/kg for liquid milk.
Monoglyceride, diglyceride, milk, liquid chromatography-mass spectrometry.
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