Bìa tạp chí

 

009bet

Simultaneous determination of picroside I and picroside II in dietary supplements by high performance liquid chromatography with PDA detector

Do Yen Nhi Nguyen Thi Anh Huong Pham Minh Phuong Trinh Yen Nhi Le Thi Thuy Vu Thi Trang
Received: 23 May 2024
Revised: 24 Jun 2024
Accepted: 25 Jun 2024
Published: 30 Sep 2024

Article Details

How to Cite
Do Yen Nhi, Nguyen Thi Anh Huong, Pham Minh Phuong, Trinh Yen Nhi, Le Thi Thuy, Vu Thi Trang. "Simultaneous determination of picroside I and picroside II in dietary supplements by high performance liquid chromatography with PDA detector". Vietnam Journal of Food Control. vol. 7, no. 3, pp. 226-236, 2024
PP
226-236
Counter
24

Main Article Content

Abstract

Picroside I and picroside II are two compounds found in the Picrorhiza Kurroa plant, which have anti-cirrhosis, antioxidant, anti-inflammatory, fever, and asthma effects, etc. They are included in the ingredients of many dietary supplement products that help protect the liver and bile, prevent oxidation, etc. Therefore, determining the content of these two active ingredients in dietary supplements is necessary to evaluate product quality. In this study, highperformance liquid chromatography (HPLC-PDA) was used to simultaneously determine picroside I and picroside II in dietary supplements. The analytes in the samples were extracted with methanol for 60 min using a horizontal shaking technique. The extract was then filtered and determined by the HPLC–PDA method with the following conditions: C18 Column (250 mm x 4.6 mm; 5 µm) and a corresponding pre-column (3.9 mm x 5 mm; 5 µm), the mobile phase consists of a solvent mixture of acetonitrile and water according to the gradient program, detection wavelength 265 nm. The method has good specificity, the calibration curve of picroside I and picroside II has a linear coefficient R2 > 0.9999 and the repeatability and recovery meet the AOAC requirements. The method limits of detection (MDL) for these two substances were 4.3 mg/kg and 3.2 mg/kg, respectively. The method was applied to simultaneously determine picroside I and picroside II in 30 different dietary supplement products. The results detected analytes in 3 samples positive with picroside I and picroside II concentrations ranging from 98.8 to 148 mg/kg.

Keywords:

picroside I, picroside II, high-performance liquid chromatography, HPLC, dietary supplements

References

[1]. P. R. Thani, Y. P. Sharma, P. Kandel and K. Nepal, “Standardization of Extraction Techniques of Picroside-I and Picroside-II from "Kutki" (Picrorhiza kurroa Royle ex Benth.),” Global Journal of Science Frontier Research: C Biological Science, vol. 8, pp. 51-56, 2018.
[2]. S. Pandit, K. Shitiz, H. Sood, P. K. Naik and R. S. Chauhan, “Expression pattern of fifteen genes of non-mevalonate (MEP) and mevalonate (MVA) pathways in different tissues of endangered medicinal herb Picrorhiza kurroa with respect to picrosides content,” Molecular Biology Reports, vol. 40, pp. 1053-1063.
[3]. S. S. Tiwari, M. M. Pandey, S. Srivastava and A. K. S. Rawat, “TLC densitometric quantification of picrosides (picroside-I and picroside-II) in Picrorhiza kurroa and its substitute Picrorhiza scrophulariiflora and their antioxidant studies,” Biomedical Chromatography, vol. 26, no. 1, pp. 61–68, 2011.
[4]. D. Upadhyay, R. P. Dash, S. Anandjiwala and M. Nivsarkar, “Comparative pharmacokinetic profiles of picrosides I and II from kutkin, Picrorhiza kurroa extract and its formulation in rats,” Fitoterapia, vol. 85, pp. 76–83, 2013.
[5]. S. Cheng, J. Huang and J. He, “Development and validation of an accurate HPLC method for the quantitative determination of picroside II in tablets,” Pharmaceutical Sciences, vol. 62, no. 8, pp. 577-9, 2007.
[6]. J. Zhu, B. Xue, B. Ma, et al., “A pre-clinical pharmacokinetic study in rats of three naturally occurring iridoid glycosides, Picroside-I, II and III, using a validated simultaneous HPLC–MS/MS assay,” Journal of Chromatography B, vol. 993-994, pp. 47-59.
[7]. F. C. Yang, S. L. Yang and L. Z. Xu, “Determination of picroside II in dog plasma by HPLC and its application in a pharmacokinetics study,” Biomedical Chromatography, vol. 19, pp. 279-84, 2005.
[8]. S. Cheng, J. Huang and J. He, “Development and validation of an accurate HPLC method for the quantitative determination of picroside II in tablets,” Die PharmazieAn International Journal of Pharmaceutical Sciences, vol. 62, pp. 577-9, 2007.
[9]. Q. Shen, W. Dong, Y. Wang, L. Gong, Z. Dai and H. Y. Cheung, “Pipette tip solidphase extraction and ultra-performance liquid chromatography/mass spectrometry based rapid analysis of picrosides from Picrorhiza scrophulariiflora,” Journal of pharmaceutical and biomedical analysis, vol. 80, pp. 136–140, 2013.
[10]. S. Zahiruddin, W. Khan, R. Nehra, M. J. Alam, M. N. Mallick, R. Parveen and S. Ahmad, “Pharmacokinetics and comparative metabolic profiling of iridoid enriched fraction of Picrorhiza kurroa – An Ayurvedic Herb,” Journal of ethnopharmacology, vol. 197, pp. 157–164, 2017.
[11]. AOAC Official Methods of Analysis, Appendix F: Guidelines for standard method performance requirements, 2012.

 Submit