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Determination of deoxynivalenol and metabolites in maize samples by LC-MS/MS

Bui Cao Tien Nguyen Thi Hong Ngoc Nguyen Quang Huy Le Thi Lan Huong Vu Ngan Binh
Received: 05 Dec 2023
Revised: 18 Jun 2024
Accepted: 25 Jun 2024
Published: 30 Sep 2024

Article Details

How to Cite
Bui Cao Tien, Nguyen Thi Hong Ngoc, Nguyen Quang Huy, Le Thi Lan Huong, Vu Ngan Binh. "Determination of deoxynivalenol and metabolites in maize samples by LC-MS/MS". Vietnam Journal of Food Control. vol. 7, no. 3, pp. 195-211, 2024
PP
195-211
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24

Main Article Content

Abstract

In Vietnam, the regulation QCVN 8-1:2011/BYT has been issued to control mycotoxin content in food products. However, there are currently no regulations or studies on their derivatives and metabolites. Deoxynivalenol-3-glucoside (D3G) and two acetylated derivatives of deoxynivalenol (DON), namely 15-acetyldeoxynivalenol (15-AcDON) and 3- acetyldeoxynivalenol (3-AcDON), have been frequently detected in naturally contaminated grains such as wheat, barley, and corn. This study developed and applied a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine deoxynivalenol (DON) and its metabolites, including deoxynivalenol-3-glucoside (D3G), 3- acetyldeoxynivalenol (3-AcDON), and 15-acetyldeoxynivalenol (15-AcDON) in maize. The instrumental conditions comprised a C18 HSS SB (150 mm × 2.1 mm × 1.7 µm) column for separation and an MS/MS detector in positive and negative electrospray ionization (ESI) source, employing multi-reactive ion monitoring mode, with an injection volume of 2 µL. The method has been fully validated and meets the performance requirements of AOAC, demonstrating high specificity. The linearity range was established from 100 µg/kg to 1000 µg/kg, with a method detection limit (MDL) of 30 µg/kg. The repeatability and recovery rates were determined to be between 1.45% to 4.31% and 90% to 110%, respectively. The validated method was applied to analyze 50 maize samples. Deoxynivalenol (DON) was detected in 10 samples (20%), while deoxynivalenol-3-glucoside (D3G), 3- acetyldeoxynivalenol (3-AcDON), and 15-acetyldeoxynivalenol (15-AcDON) were not detected in any of the samples.

Keywords:

metabolites, DON, D3G, 3-AcDON, 15-AcDON

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