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Simultaneous determination of several forms of phosphate in food by ion exchange chromatography

Do Thi Hong Thuy Nguyen Nhu Thuong Vu Thi Trang Le Thi Hong Hao Le Thi Thuy Le Viet Ngan Luu Thi Huyen Trang Cao Cong Khanh Dinh Viet Chien Le Thi Phuong Thao Nguyen Thi Anh Huong
Received: 14 Apr 2024
Revised: 13 May 2024
Accepted: 13 May 2024
Published: 29 Jun 2024

Article Details

How to Cite
Do Thi Hong Thuy, Nguyen Nhu Thuong, Vu Thi Trang, Le Thi Hong Hao, Le Thi Thuy, Le Viet Ngan, Luu Thi Huyen Trang, Cao Cong Khanh, Dinh Viet Chien, Le Thi Phuong Thao, Nguyen Thi Anh Huong. "Simultaneous determination of several forms of phosphate in food by ion exchange chromatography". Vietnam Journal of Food Control. vol. 7, no. 2 (en), pp. 132-143, 2024

Main Article Content


The phosphate group is a group of food additives commonly used for moisturizing, improving the structure, and retaining the color and flavor of products prepared from meat, fish, milk, baked goods, and beverages. A reliable and fast ion exchange chromatography method developed for the simultaneous determination of several forms of phosphate (orthophosphoric acid; pyrophosphate, triphosphate, and hexametaphosphate) in food. Samples were extracted with deionized water at a temperature of 25 ± 5oC for 30 minutes. The extracts were determined by ion exchange chromatography under the conditions: Dionex IonPacTM AS11 column (4 x 250 mm, 9 µm) and Dionex IonPaxTM AG11 column (4 x 50 mm, 9 µm) with the gradient program of KOH concentration from 20 mM to 80 mM. The flow rate is 1 mL/min. The method has good specificity, the calibration curves of the four substances have correlation coefficient values R2 > 0.9999, repeatability and recovery meet the requirements of AOAC. The detection limits (LOD) and quantification limit (LOQ) of each substance in the phosphate group are 12 mg/kg and 40 mg/kg, respectively. The method was applied to simultaneously determine several forms of phosphate in 50 food samples: 15/50 samples detected pyrophosphate, 2/50 samples detected triphosphate, 18/50 samples detected hexametaphosphate and 44/50 samples detected ortho-phosphoric acid, 6/50 samples not detected any form of phosphate. The concentration of phosphate group in detected samples varied from 67.9 mg/kg to 2499 mg/kg.


polyphosphate, phosphate, ion exchange chromatography.


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