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Detection of alpha-amanitin encoding gene utilizing real-time PCR technique

Le Vinh Hoa Pham Van Quan Le Thanh Long Pham Thi Loan Nguyen Thi Thanh Huyen
Received: 14 Jan 2024
Revised: 08 Apr 2024
Accepted: 09 Apr 2024
Published: 29 Jun 2024

Article Details

How to Cite
Le Vinh Hoa, Pham Van Quan, Le Thanh Long, Pham Thi Loan, Nguyen Thi Thanh Huyen. "Detection of alpha-amanitin encoding gene utilizing real-time PCR technique". Vietnam Journal of Food Control. vol. 7, no. 2 (en), pp. 111-123, 2024
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111-123
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Main Article Content

Abstract

Given the limited taxonomy of toxic fungi as well as the large number of mushroom species, identification of mycotoxins in mushroom samples can accelerate information gathering and progress, while increasing Enhanced testing accuracy of testing. In that situation, it is necessary to research methods to identify toxin-carrying genes in mushrooms, to help monitor and classify poisonous mushrooms, disseminate and increase people's awareness of food safety. done faster and more accurately. The research team succeeded in developing a real-time PCR method to detect the toxin alpha-amanitin gene on mushrooms with a detection limit of 101 copies/µL. The study has fully verified the correct parameters, 100% accuracy, and specificity.

Keywords:

Alpha-amanitin, poisonous mushrooms, food poisoning, real-time PCR.

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