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Producing positive control materials for template in PCR testing to detect botulinum neurotoxin types A and B genes

Ninh Thi Hanh Le Vinh Hoa Tran Hong Ba Pham Van Quan Le Thanh Long Pham Thi Loan
Received: 22 Dec 2023
Revised: 07 Mar 2024
Accepted: 21 Mar 2024
Published: 31 Mar 2024

Article Details

How to Cite
Ninh Thi Hanh, Le Vinh Hoa, Tran Hong Ba, Pham Van Quan, Le Thanh Long, Pham Thi Loan. "Producing positive control materials for template in PCR testing to detect botulinum neurotoxin types A and B genes". Vietnam Journal of Food Control. vol. 7, no. 1, pp. 53-67, 2024
PP
53-67
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156

Main Article Content

Abstract

Botulinum neurotoxin (BoNT) is produced by the bacterium Clostridium botulinum and some other bacterial strains such as C. butyricum and C. baratii. It is considered the strongest toxin known to humans and can cause botulism. Botulinum neurotoxin can be fatal in humans and most botulism cases are caused by type A and B toxins. In this study, the research team used C. botulinum strains isolated from botulism poisoning cases in Vietnam, which were identified to produce type A and B toxins, from which we successfully transformed plasmids carrying genes specific for BoNT type A and type B genes based on the reference gene sequence according to TCVN 11395:2016. These plasmids were successfully used as template DNA for PCR reactions. In the PCR reaction, plasmids used to transform genes producing botulinum toxin types A and B have a detection limit of 102 copies/µL. The study has fully verified the validation parameters, with 100% accuracy and specificity.

Keywords:

neurotoxin, Clostridium botulinum, food poisoning, PCR.

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