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Quantification of lactoferrin in dietary supplements using a Heparin affinity clean-up column by HPLC PDA detector

Nguyen Quang Hung Tran Ngoc Lan Kieu Van Anh Doan Thu Thuong Nguyen Van Khoa Pham Thanh Mai Thai Nguyen Thao Linh Nguyen Thi Van Anh Cao Cong Khanh Mac Thi Thanh Hoa
Received: 09 Sep 2023
Revised: 07 Dec 2023
Accepted: 09 Dec 2023
Published: 29 Dec 2023

Article Details

How to Cite
Nguyen Quang Hung, Tran Ngoc Lan, Kieu Van Anh, Doan Thu Thuong, Nguyen Van Khoa, Pham Thanh Mai, Thai Nguyen Thao Linh, Nguyen Thi Van Anh, Cao Cong Khanh, Mac Thi Thanh Hoa. "Quantification of lactoferrin in dietary supplements using a Heparin affinity clean-up column by HPLC PDA detector". Vietnam Journal of Food Control. vol. 6, no. 4 (en), pp. 417-431, 2023
PP
417-431
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200

Main Article Content

Abstract

The use of dietary supplements containing lactoferrin is a growing trend. As a result, there is a need for a simple and reliable analytical method to determine the content of lactoferrin in these supplements to ensure food quality. In this study, an HPLC method was developed and validated for determining lactoferrin in dietary supplements using a HiTrap Heparin clean-up column in combination with HPLC. The study involved selecting optimal conditions for extracting lactoferrin from the sample matrix, followed by chromatography conditions using a C4 column (150 mm × 4.6 mm, 3.5 µm). The limit of detection (LOD) and limit of quantification (LOQ) were determined, with the achieved values being 1.2 mg/100g and 4.0 mg/100g, respectively. The method has shown good recovery (99.5–103.4%) and precision (RSD 1.76–3.44%), meeting the method performance requirements outlined in AOAC SMPR 2020.005. The validated method was applied to determine the contents of lactoferrin in dietary supplement samples.

Keywords:

lactoferrin, dietary supplement, HPLC, affinity column, clean-up, Heparin

References

[1]. L. Adlerova, A. Bartoskova, M. Faldyna, "Lactoferrin: a review", Veterinarni Medicina, vol. 53, no. 9, pp. 457–468, 2008
[2]. L. Sanchez, M. Calvo, J. H. Brock, "Biological role of lactoferrin", Archives of Disease in Childhood, vol. 67, pp. 657-661, 1992
[3]. X. Cao, Y. Ren, Q. Lu, et al., "Lactoferrin: A glycoprotein that plays an active role in human health", Frontiers in Nutrition, 9:1018336, 2023
[4]. Z. Yang, R. Jiang, Q. Chen et al., "Concentration of lactoferrin in human milk and its variation during lactation in different Chinese populations", Nutrients, vol. 10, no. 9, 2018.
[5]. R. Sharma, "Whey proteins in functional foods", Whey Proteins, pp. 637–663, 2019.
[6]. S. V. Hetherington, J. K. Spitznagel, P. G. Quie, "An enzyme-linked immunoassay (ELISA) for measurement of lactoferrin", Journal of Immunological Methods, 65 (1-2), 183–190, 1984.
[7]. M. Tomassetti, E. Martini, L. Campanella, et al., "Lactoferrin determination using flow or batch immunosensor surface plasmon resonance: Comparison with amperometric and screen-printed immunosensor methods", Sensors Actuators B-Chemical, vol. 179, no. 2, pp. 215–225.
[8]. J. Li, X. Ding, Y. Chen, et al, "Determination of bovine lactoferrin in infant formula by capillary electrophoresis with ultraviolet detection", Journal of Chromatography A, vol. 1244, pp. 178-183, 2012
[9]. J. Zhang, S. Lai, Z. Cai, et al., "Determination of bovine lactoferrin in dairy products by ultra-high performance liquid chromatography-tandem mass spectrometry based on tryptic signature peptides employing an isotope-labeled winged peptide as internal standard", Analytica Chimica Acta, vol. 829, pp. 33-39, 2014
[10]. K. P. Palmano, D. F. Elgar, “Detection and quantitation of lactoferrin in bovine whey samples by reversed-phase high-performance liquid chromatography on polystyrene-divinylbenzene”, Journal of Chromatography A, vol. 947, no. 2, pp. 307–311, 2002
[11]. I. Capila, R. J. Linhardt, "Heparin–Protein Interactions", Angewandte Chemie International Edition, vol. 41, no. 3, pp. 390–412, 2002
[12]. W.B. Ounis, S.F. Gauthier, S.L. Turgeon, et al., (2008), “Separation of minor protein components from whey protein isolates by heparin affinity chromatography”, International Dairy Journal, vol. 18, Issues. 10–11, pp. 1043–1050, 2008
[13]. M. Chen, F. Wen, Y. Zhang, et al., "Determination of native lactoferrin in milk by HPLC on HiTrapTM Heparin HP column", Food Analytical Methods, vol. 12, no.11, pp. 2518–2526, 2019.
[14]. Y. Zhang, F. Lou, W. Wu, et al., "Determination of Bovine Lactoferrin in Food by HPLC with a Heparin Affinity Column for Sample Preparation", Journal of AOAC International, vol. 100, no. 1, pp. 133-138, 2017.
[15]. S. Zou, C. E. Magura, W. L. Hurley, "Effect of pH and some cations on binding of heparin to lactoferrin", Comparative biochemistry and physiology B, vol. 103, no. 4, pp. 889-895, 1992
[16]. Cytiva, "HiTrap Heparin HL, 1 mL and 5 mL, HiTrap affinity columns", Instructions for Use
[17]. AOAC, "Appendix F: Guidelines for standard method performance requirements", AOAC Official Methods of Analysis, 2012
[18]. Standard Method Performance Requirements SMPR 2020.005, "Standard Method Performance Requirements (SMPRs) for Determination of Bovine Lactoferrin in Infant and Adult/Pediatric Nutritional Formula", Official Methods of Analysis, AOAC International, 2020.

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