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Quantification of lactoferrin in dietary supplements using a Heparin affinity clean-up column by HPLC PDA detector

Nguyen Quang Hung Tran Ngoc Lan Kieu Van Anh Doan Thu Thuong Nguyen Van Khoa Pham Thanh Mai Thai Nguyen Thao Linh Nguyen Thi Van Anh Cao Cong Khanh Mac Thi Thanh Hoa
Received: 09 Sep 2023
Revised: 07 Dec 2023
Accepted: 09 Dec 2023
Published: 29 Dec 2023

Article Details

How to Cite
Nguyen Quang Hung, Tran Ngoc Lan, Kieu Van Anh, Doan Thu Thuong, Nguyen Van Khoa, Pham Thanh Mai, Thai Nguyen Thao Linh, Nguyen Thi Van Anh, Cao Cong Khanh, Mac Thi Thanh Hoa. "Quantification of lactoferrin in dietary supplements using a Heparin affinity clean-up column by HPLC PDA detector". Vietnam Journal of Food Control. vol. 6, no. 4 (en), pp. 417-431, 2023

Main Article Content


The use of dietary supplements containing lactoferrin is a growing trend. As a result, there is a need for a simple and reliable analytical method to determine the content of lactoferrin in these supplements to ensure food quality. In this study, an HPLC method was developed and validated for determining lactoferrin in dietary supplements using a HiTrap Heparin clean-up column in combination with HPLC. The study involved selecting optimal conditions for extracting lactoferrin from the sample matrix, followed by chromatography conditions using a C4 column (150 mm × 4.6 mm, 3.5 µm). The limit of detection (LOD) and limit of quantification (LOQ) were determined, with the achieved values being 1.2 mg/100g and 4.0 mg/100g, respectively. The method has shown good recovery (99.5–103.4%) and precision (RSD 1.76–3.44%), meeting the method performance requirements outlined in AOAC SMPR 2020.005. The validated method was applied to determine the contents of lactoferrin in dietary supplement samples.


lactoferrin, dietary supplement, HPLC, affinity column, clean-up, Heparin


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