There is an increasing demand from consumers for natural and minimally processed foods. Therefore, developing and producing effective natural antimicrobial substances for food preservation is of great interest. This work aimed to investigate the effect of growth conditions on the maximum activity of antibacterial substances production by Lactiplantibacillus sp. NCL33 from Nem chua, a traditional fermented meat. The results revealed that the antibacterial substances exhibited a broad spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria. The antibacterial activity of NCL33 was optimized with MRS broth supplemented with 20 g/L glucose. The suitable nitrogen source for antibacterial biosynthesis was yeast extract with a concentration of 25 g/L. Moreover, the inoculated temperature and initial pH significantly influenced the antimicrobial activity. The maximum antimicrobial activity of NCL33 was obtained at 30°C and pH 7.0.
Lactiplantibacillus sp., antimicrobial activity, cultivation conditions.
. E. Claeys, S. De Smet, A. Balcaen, K. Raes, and D. Demeyer, “Quantification of fresh meat peptides by SDS-PAGE in relation to ageing time and taste intensity,” Meat Science, vol. 67, pp. 281-288, 2004.
. F. Leroy, J. Verluyten, and L. de Vuyst, “Functional meat starter cultures for improved sausages fermentation,” International Journal Food Microbiology, vol. 106, pp. 270-285, 2006.
. Y. Zhang, J. Z. Liu, J. S. Huang, and Z. W. Mao, “Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis,” Journal of Biotechnology, vol. 148, pp. 139-143, 2010.
. S. Erkkila, E. Peteja, S. Eerola, L. Lilleberg, T. Mattila-Sandholm, and M. L. Suihko, “Flavour profiles of dry sausages fermented by selected novel meat starter cultures,” Meat Science, vol. 58, pp. 111-116, 2001.
. S. D. Todorov, B. D. G. M. Franco, and I. J. Wiid, “In vitro study of beneficial properties and safety of lactic acid bacteria isolated from Portuguese fermented meat products,” Beneficial Microbes, vol. 5, pp. 351-366, 2013.
. N. H. Huong, D. T. Vy, T. L. Chau, and D. K. Nhu, “Selective isolation of lactic acid bacteria from traditional fermented meat (Nem chua) as starter strains of probiotic fermented meat (Nem chua)," Proceedings of the Conference on Environmental Science and Biotechnology, 2011, pp. 149-157.
. J. Miao, M. Xu, H. Guo, L. He, X. Gao, C. DiMarco-Crook, H. Xiao, and Y. Cao, “Optimization of culture conditions for the production of antimicrobial substances by probiotic Lactobacillus paracasei subsp. Tolerans FX-6,” Journal of Functional Foods, vol 18, pp. 244-253, 2015.
[8.] T. L. Yue, J. J. Pei, and Y. H. Yuan, “Purification and characterization of anti - Alicyclobacillus bacteriocin produced by Lactobacillus rhamnosus,” Journal of Food Protection, vol. 76, pp. 1575-1581, 2013.
. S. Ammor, G. Tauveron, E. Dufour, and I. Chevallier, “Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility. Screening and characterization of the antibacterial compounds,” Food Control, vol. 17, pp. 454-461, 2005.
. T. T. Phan, T. H. Do, L. H. Tran, and D. H. Hoang, “Isolation and characterization of Lactobacillus plantarum H1.40 from Vietnamese traditional fermented meat (Nem chua),” In: Proceedings of Regional Symposium on Chemical Engineering, 30 November-2 December 2005, Hanoi, Vietnam.
. F. B. Elegado, A. C. L. Opina, C. G. B. Banaay, I. F. and Dalmacio, “Purification and characterization of novel bacteriocins from lactic acid bacteria isolated from Philippine fermented rice-shrimp or rice-fish mixtures,” The Philippine Agricultural Scientist, vol. 86, pp. 65-74, 2003.
. A. Maldonado, R. Jimenez-Diaz, J. L. and Ruiz-Barba, “Induction of plantaricin production in Lactobacillus plantarum NC8 after co-culture with specific Gram-positive bacteria is mediated by an auto-induction mechanism,” Journal of Bacteriology, vol. 186, pp. 1556-1564, 2004.
. M. Calderon, G. Loiseau, J. P. Guyot, “Nutritional requirements and simplified cultivation medium to study growth and energetics of a sourdough lactic acid bacterium Lactobacillus fermentum Ogi E1 during heterolactic fermentation of starch,” Journal Applied Microbiology, vol. 90, pp. 508-516, 2001.
. E. Parlindungan, C. Dekiwadia, O. A. H. Jones, “Factors that influence growth and bacteriocin production in Lactiplantibacillus plantarum B21”, Process Biochemistry, vol 107, pp. 18-26, 2021.
. I. M. Aasen, T. Moretro, T. Katla, L. Axelsson, I. Storro, “Influence of complex nutrients, temperature and pH on bacteriocin production by Lactobacillus sakei CCUG 42687,” Applied Microbiology Biotechnology, vol. 53, pp. 159-166, 2000.
. T. Lechiancole, A. Ricciardi, E. Parente, “Optimization of media and fermentation conditions for the growth of Lactobacillus sakei,” Annals Microbiology, vol. 52, pp. 257-274, 2002.
. S. D. Todorov, L. M. Dicks, “Effect of medium components on bacteriocin production by Lactobacillus plantarum strains ST23LD and ST341LD, isolated from spoiled olive brine,” Microbiology Research, vol. 161, pp. 102-108, 2006.
. S. D. Todorov, C. A. Van Reenen, L. M. T. Dicks, “Optimization of bacteriocin production by Lactobacillus plantarum ST13BR, a strain isolated from barley beer,” The Journal of General and Applied Microbiology, vol. 50, pp. 149-57, 2004.
. S. D. Todorov, P. Hob, M. Vaz-Velho, and L. M. T. Dicks, “Characterization of bacteriocins produced by two strains of Lactobacillus plantarum isolated from Beloura and Chouriço, traditional pork products from Portugal,” Meat Sciences, vol. 84, pp. 334-343, 2010.
[20.] E. Yang, L. Fan, J. Yan, Y. Jiang, C. Doucette, S. Fillmore, and B. Walker, “Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria,” AMB Express, vol. 8, 2018.
. M. Mataragas, J. Metaxopoulos, M. Galiotou, and E. H. Drosinos, “Influence of pH and temperature on growth and bacteriocin production by Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442,” Meat Science, vol. 64, pp. 265-271, 2003.
. F. Ibrahim, S. B. Zafar, A. Aman, S. A. Ul Qader, and A. Ansari, “Improvement of Lactobacillus plantarum for the enhanced production of bacteriocin like inhibitory substance using combinatorial approach,” Biocatalysis Agricultural Biotechnology, vol. 22, 101386, 2019.